Bioanalysis, volume 10, issue 19, pages 1591-1608

Quantification of phenolic acid metabolites in humans by LC–MS: a structural and targeted metabolomics approach

Mark E Obrenovich 1, 2, 3, 4
Curtis J Donskey 5, 6
Isaac T Schiefer 4
Rodolfo Bongiovanni 2
Ling Li 7
George E Jaskiw 8, 9
1
 
Pathology & Laboratory Medicine Service, Louis Stokes Cleveland Department of Veteran's Affairs Medical Center, Cleveland, OH, 44106, USA
2
 
Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH, 44106, USA
5
 
Infectious Disease Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH, 44106, USA
8
 
Psychiatry Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH, 44106, USA
Publication typeJournal Article
Publication date2018-10-08
Journal: Bioanalysis
scimago Q2
SJR0.430
CiteScore3.3
Impact factor1.9
ISSN17576180, 17576199
General Medicine
Clinical Biochemistry
Analytical Chemistry
General Pharmacology, Toxicology and Pharmaceutics
Medical Laboratory Technology
Abstract

Aim: Co-metabolism between a human host and the gastrointestinal microbiota generates many small phenolic molecules such as 3-hydroxy-3-(3-hydroxyphenyl)propanoic acid (3,3-HPHPA), which are reported to be elevated in schizophrenia and autism. Characterization of these chemicals, however, has been limited by analytic challenges. Methodology/results: We applied HPLC to separate and quantify over 50 analytes, including multiple structural isomers of 3,3-HPHPA in human cerebrospinal fluid, serum and urine. Confirmation of identity was provided by NMR, by MS and other detection methods. The highly selective methods support rapid quantification of multiple metabolites and exhibit superior chromatographic behavior. Conclusion: An improved ultra-HPLC–MS/MS and structural approaches can accurately quantify 3,3-HPHPA and related analytes in human biological matrices.

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