Open Access
Cells, volume 9, issue 4, pages 1061
Modification of Adenosine196 by Mettl3 Methyltransferase in the 5'-External Transcribed Spacer of 47S Pre-rRNA Affects rRNA Maturation
Sergeeva Olga
1
,
Sergeev Philipp
1
,
Melnikov Pavel A
2
,
Dontsova Olga
1, 3
,
Zatsepin Timofei S.
1, 3
Publication type: Journal Article
Publication date: 2020-04-24
PubMed ID:
32344536
General Medicine
Abstract
Ribosome biogenesis is among the founding processes in the cell. During the first stages of ribosome biogenesis, polycistronic precursor of ribosomal RNA passes complex multistage maturation after transcription. Quality control of preribosomal RNA (pre-rRNA) processing is precisely regulated by non-ribosomal proteins and structural features of pre-rRNA molecules, including modified nucleotides. However, many participants of rRNA maturation are still unknown or poorly characterized. We report that RNA m6A methyltransferase Mettl3 interacts with the 5′ external transcribed spacer (5′ETS) of the 47S rRNA precursor and modifies adenosine 196. We demonstrated that Mettl3 knockdown results in the increase of pre-rRNA processing rates, while intracellular amounts of rRNA processing machinery components (U3, U8, U13, U14, and U17 small nucleolar RNA (snoRNA)and fibrillarin, nucleolin, Xrn2, and rrp9 proteins), rRNA degradation rates, and total amount of mature rRNA in the cell stay unchanged. Increased efficacy of pre-rRNA cleavage at A’ and A0 positions led to the decrease of 47S and 45S pre-rRNAs in the cell and increase of mature rRNA amount in the cytoplasm. The newly identified conserved motif DRACH sequence modified by Mettl3 in the 5′-ETS region is found and conserved only in primates, which may suggest participation of m6A196 in quality control of pre-rRNA processing at initial stages demanded by increased complexity of ribosome biogenesis.
Citations by journals
|
1
|
|
|
Nucleic Acids Research
|
Nucleic Acids Research
1 publication, 10%
|
|
Frontiers in Molecular Neuroscience
|
Frontiers in Molecular Neuroscience
1 publication, 10%
|
|
International Journal of Molecular Sciences
|
International Journal of Molecular Sciences
1 publication, 10%
|
|
Biology
|
Biology
1 publication, 10%
|
|
Frontiers in Genetics
|
Frontiers in Genetics
1 publication, 10%
|
|
Frontiers in Cell and Developmental Biology
|
Frontiers in Cell and Developmental Biology
1 publication, 10%
|
|
Frontiers in Immunology
|
Frontiers in Immunology
1 publication, 10%
|
|
Signal Transduction and Targeted Therapy
|
Signal Transduction and Targeted Therapy
1 publication, 10%
|
|
Biological Chemistry
|
Biological Chemistry
1 publication, 10%
|
|
Science of the Total Environment
|
Science of the Total Environment
1 publication, 10%
|
|
1
|
Citations by publishers
|
1
2
3
4
|
|
|
Frontiers Media S.A.
|
Frontiers Media S.A.
4 publications, 40%
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 20%
|
|
Oxford University Press
|
Oxford University Press
1 publication, 10%
|
|
Springer Nature
|
Springer Nature
1 publication, 10%
|
|
Walter de Gruyter
|
Walter de Gruyter
1 publication, 10%
|
|
Elsevier
|
Elsevier
1 publication, 10%
|
|
1
2
3
4
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2020,2021,2022,2023],"ids":[0,0,0,0],"codes":[0,0,0,0],"imageUrls":["","","",""],"datasets":[{"label":"Citations number","data":[1,4,2,3],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["10","40","20","30"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Nucleic Acids Research","Frontiers in Molecular Neuroscience","International Journal of Molecular Sciences","Biology","Frontiers in Genetics","Frontiers in Cell and Developmental Biology","Frontiers in Immunology","Signal Transduction and Targeted Therapy","Biological Chemistry","Science of the Total Environment"],"ids":[23904,20786,14627,24144,13242,12128,1699,10186,10443,2679],"codes":[0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/3SpVxcYL33bOvPq4sHxJLH2NeKNeDloahSUpNiO4_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp"],"datasets":[{"label":"","data":[1,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[10,10,10,10,10,10,10,10,10,10],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Frontiers Media S.A.","Multidisciplinary Digital Publishing Institute (MDPI)","Oxford University Press","Springer Nature","Walter de Gruyter","Elsevier"],"ids":[208,202,19,8,4,17],"codes":[0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/3SpVxcYL33bOvPq4sHxJLH2NeKNeDloahSUpNiO4_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp"],"datasets":[{"label":"","data":[4,2,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[40,20,10,10,10,10],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Sergeeva O. et al. Modification of Adenosine196 by Mettl3 Methyltransferase in the 5'-External Transcribed Spacer of 47S Pre-rRNA Affects rRNA Maturation // Cells. 2020. Vol. 9. No. 4. p. 1061.
GOST all authors (up to 50)
Copy
Sergeeva O., Sergeev P., Melnikov P. A., Prikazchikova T., Dontsova O., Zatsepin T. S. Modification of Adenosine196 by Mettl3 Methyltransferase in the 5'-External Transcribed Spacer of 47S Pre-rRNA Affects rRNA Maturation // Cells. 2020. Vol. 9. No. 4. p. 1061.
Cite this
RIS
Copy
TY - JOUR
DO - 10.3390/cells9041061
UR - https://doi.org/10.3390%2Fcells9041061
TI - Modification of Adenosine196 by Mettl3 Methyltransferase in the 5'-External Transcribed Spacer of 47S Pre-rRNA Affects rRNA Maturation
T2 - Cells
AU - Sergeeva, Olga
AU - Melnikov, Pavel A
AU - Prikazchikova, Tatiana
AU - Dontsova, Olga
AU - Sergeev, Philipp
AU - Zatsepin, Timofei S.
PY - 2020
DA - 2020/04/24 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 1061
IS - 4
VL - 9
PMID - 32344536
SN - 2073-4409
ER -
Cite this
BibTex
Copy
@article{2020_Sergeeva,
author = {Olga Sergeeva and Pavel A Melnikov and Tatiana Prikazchikova and Olga Dontsova and Philipp Sergeev and Timofei S. Zatsepin},
title = {Modification of Adenosine196 by Mettl3 Methyltransferase in the 5'-External Transcribed Spacer of 47S Pre-rRNA Affects rRNA Maturation},
journal = {Cells},
year = {2020},
volume = {9},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {apr},
url = {https://doi.org/10.3390%2Fcells9041061},
number = {4},
pages = {1061},
doi = {10.3390/cells9041061}
}
Cite this
MLA
Copy
Sergeeva, Olga, et al. “Modification of Adenosine196 by Mettl3 Methyltransferase in the 5'-External Transcribed Spacer of 47S Pre-rRNA Affects rRNA Maturation.” Cells, vol. 9, no. 4, Apr. 2020, p. 1061. https://doi.org/10.3390%2Fcells9041061.