Chemical Reviews, volume 117, issue 2, pages 758-795

Photoinduced chemistry in fluorescent proteins: Curse or blessing?

Acharya Arbind ¹

Bogdanov Alexey M ^{2, 3}

Grigorenko Bella ^{4, 5}

Bravaya Ksenia B. ⁶

Nemukhin Alexander ^{4, 5}

Lukyanov Konstantin A. ^{2, 3}

Krylov Anna I. ¹

Hide authors affiliations Show authors affiliations: 6 affiliations

Department of Chemistry, University of Southern California, Los Angeles, California 90089-0482, United States |

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia |

Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia |

⁴

Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia |

⁵

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia |

⁶

Department of Chemistry, Boston University, Boston, Massachusetts United States |

Publication type: Journal Article

Publication date: 2016-10-18

American Chemical Society (ACS)

Journal: Chemical Reviews

Quartile SCImago

Quartile WOS

Impact factor: 62.1

ISSN: 00092665, 15206890

DOI: 10.1021/acs.chemrev.6b00238

Copy DOI

General Chemistry

Abstract

Photoinduced reactions play an important role in the photocycle of fluorescent proteins from the green fluorescent protein (GFP) family. Among such processes are photoisomerization, photooxidation/photoreduction, breaking and making of covalent bonds, and excited-state proton transfer (ESPT). Many of these transformations are initiated by electron transfer (ET). The quantum yields of these processes vary significantly, from nearly 1 for ESPT to 10-4-10-6 for ET. Importantly, even when quantum yields are relatively small, at the conditions of repeated illumination the overall effect is significant. Depending on the task at hand, fluorescent protein photochemistry is regarded either as an asset facilitating new applications or as a nuisance leading to the loss of optical output. The phenomena arising due to phototransformations include (i) large Stokes shifts, (ii) photoconversions, photoactivation, and photoswitching, (iii) phototoxicity, (iv) blinking, (v) permanent bleaching, and (vi) formation of long-lived intermediates. The focus of this review is on the most recent experimental and theoretical work on photoinduced transformations in fluorescent proteins. We also provide an overview of the photophysics of fluorescent proteins, highlighting the interplay between photochemistry and other channels (fluorescence, radiationless relaxation, and intersystem crossing). The similarities and differences with photochemical processes in other biological systems and in dyes are also discussed.

By date By citations

Citations by journals

	2 4 6 8 10 12 14 16 18
Journal of Physical Chemistry B	Journal of Physical Chemistry B, 18, 9.05% Journal of Physical Chemistry B 18 publications, 9.05%
Journal of Physical Chemistry Letters	Journal of Physical Chemistry Letters, 10, 5.03% Journal of Physical Chemistry Letters 10 publications, 5.03%
Journal of the American Chemical Society	Journal of the American Chemical Society, 9, 4.52% Journal of the American Chemical Society 9 publications, 4.52%
Physical Chemistry Chemical Physics	Physical Chemistry Chemical Physics, 9, 4.52% Physical Chemistry Chemical Physics 9 publications, 4.52%
Journal of Chemical Theory and Computation	Journal of Chemical Theory and Computation, 9, 4.52% Journal of Chemical Theory and Computation 9 publications, 4.52%
Chemical Science	Chemical Science, 6, 3.02% Chemical Science 6 publications, 3.02%
Chemical Reviews	Chemical Reviews, 6, 3.02% Chemical Reviews 6 publications, 3.02%
Journal of Chemical Physics	Journal of Chemical Physics, 5, 2.51% Journal of Chemical Physics 5 publications, 2.51%
Methods and Applications in Fluorescence	Methods and Applications in Fluorescence, 4, 2.01% Methods and Applications in Fluorescence 4 publications, 2.01%
Nature Communications	Nature Communications, 4, 2.01% Nature Communications 4 publications, 2.01%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 4, 2.01% International Journal of Molecular Sciences 4 publications, 2.01%
Angewandte Chemie	Angewandte Chemie, 4, 2.01% Angewandte Chemie 4 publications, 2.01%
Angewandte Chemie - International Edition	Angewandte Chemie - International Edition, 4, 2.01% Angewandte Chemie - International Edition 4 publications, 2.01%
Photochemistry and Photobiology	Photochemistry and Photobiology, 3, 1.51% Photochemistry and Photobiology 3 publications, 1.51%
Faraday Discussions	Faraday Discussions, 3, 1.51% Faraday Discussions 3 publications, 1.51%
Journal of Physical Chemistry A	Journal of Physical Chemistry A, 3, 1.51% Journal of Physical Chemistry A 3 publications, 1.51%
ACS Chemical Biology	ACS Chemical Biology, 3, 1.51% ACS Chemical Biology 3 publications, 1.51%
Journal of Chemical Information and Modeling	Journal of Chemical Information and Modeling, 2, 1.01% Journal of Chemical Information and Modeling 2 publications, 1.01%
Chemical Physics Letters	Chemical Physics Letters, 2, 1.01% Chemical Physics Letters 2 publications, 1.01%
Frontiers in Molecular Biosciences	Frontiers in Molecular Biosciences, 2, 1.01% Frontiers in Molecular Biosciences 2 publications, 1.01%
Molecules	Molecules, 2, 1.01% Molecules 2 publications, 1.01%
Communications Chemistry	Communications Chemistry, 2, 1.01% Communications Chemistry 2 publications, 1.01%
Journal of Luminescence	Journal of Luminescence, 2, 1.01% Journal of Luminescence 2 publications, 1.01%
Journal of Photochemistry and Photobiology A: Chemistry	Journal of Photochemistry and Photobiology A: Chemistry, 2, 1.01% Journal of Photochemistry and Photobiology A: Chemistry 2 publications, 1.01%
Annual Review of Physical Chemistry	Annual Review of Physical Chemistry, 2, 1.01% Annual Review of Physical Chemistry 2 publications, 1.01%
Journal of Computational Chemistry	Journal of Computational Chemistry, 2, 1.01% Journal of Computational Chemistry 2 publications, 1.01%
Advanced Science	Advanced Science, 2, 1.01% Advanced Science 2 publications, 1.01%
European Journal of Organic Chemistry	European Journal of Organic Chemistry, 2, 1.01% European Journal of Organic Chemistry 2 publications, 1.01%
Chemistry - An Asian Journal	Chemistry - An Asian Journal, 2, 1.01% Chemistry - An Asian Journal 2 publications, 1.01%
	2 4 6 8 10 12 14 16 18

Citations by publishers

	10 20 30 40 50 60 70
American Chemical Society (ACS)	American Chemical Society (ACS), 65, 32.66% American Chemical Society (ACS) 65 publications, 32.66%
Wiley	Wiley, 31, 15.58% Wiley 31 publications, 15.58%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 25, 12.56% Royal Society of Chemistry (RSC) 25 publications, 12.56%
Elsevier	Elsevier, 20, 10.05% Elsevier 20 publications, 10.05%
Springer Nature	Springer Nature, 11, 5.53% Springer Nature 11 publications, 5.53%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 7, 3.52% Multidisciplinary Digital Publishing Institute (MDPI) 7 publications, 3.52%
Pleiades Publishing	Pleiades Publishing, 5, 2.51% Pleiades Publishing 5 publications, 2.51%
IOP Publishing	IOP Publishing, 5, 2.51% IOP Publishing 5 publications, 2.51%
American Institute of Physics (AIP)	American Institute of Physics (AIP), 5, 2.51% American Institute of Physics (AIP) 5 publications, 2.51%
Taylor & Francis	Taylor & Francis, 4, 2.01% Taylor & Francis 4 publications, 2.01%
Frontiers Media S.A.	Frontiers Media S.A., 3, 1.51% Frontiers Media S.A. 3 publications, 1.51%
Annual Reviews	Annual Reviews, 3, 1.51% Annual Reviews 3 publications, 1.51%
American Association for the Advancement of Science (AAAS)	American Association for the Advancement of Science (AAAS), 2, 1.01% American Association for the Advancement of Science (AAAS) 2 publications, 1.01%
American Physical Society (APS)	American Physical Society (APS), 1, 0.5% American Physical Society (APS) 1 publication, 0.5%
The Chemical Society of Japan	The Chemical Society of Japan, 1, 0.5% The Chemical Society of Japan 1 publication, 0.5%
Pirogov Russian National Research Medical University	Pirogov Russian National Research Medical University, 1, 0.5% Pirogov Russian National Research Medical University 1 publication, 0.5%
American Society for Cell Biology (ASCB)	American Society for Cell Biology (ASCB), 1, 0.5% American Society for Cell Biology (ASCB) 1 publication, 0.5%
Oxford University Press	Oxford University Press, 1, 0.5% Oxford University Press 1 publication, 0.5%
	10 20 30 40 50 60 70

We do not take into account publications that without a DOI.
Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
Statistics recalculated weekly.

{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2017,2018,2019,2020,2021,2022,2023,2024],"ids":[0,0,0,0,0,0,0,0],"codes":[0,0,0,0,0,0,0,0],"imageUrls":["","","","","","","",""],"datasets":[{"label":"Citations number","data":[20,33,33,27,28,27,22,9],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["10.05","16.58","16.58","13.57","14.07","13.57","11.06","4.52"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Journal of Physical Chemistry B","Journal of Physical Chemistry Letters","Journal of the American Chemical Society","Physical Chemistry Chemical Physics","Journal of Chemical Theory and Computation","Chemical Science","Chemical Reviews","Journal of Chemical Physics","Methods and Applications in Fluorescence","Nature Communications","International Journal of Molecular Sciences","Angewandte Chemie","Angewandte Chemie - International Edition","Photochemistry and Photobiology","Faraday Discussions","Journal of Physical Chemistry A","ACS Chemical Biology","Journal of Chemical Information and Modeling","Chemical Physics Letters","Frontiers in Molecular Biosciences","Molecules","Communications Chemistry","Journal of Luminescence","Journal of Photochemistry and Photobiology A: Chemistry","Annual Review of Physical Chemistry","Journal of Computational Chemistry","Advanced Science","European Journal of Organic Chemistry","Chemistry - An Asian Journal"],"ids":[14990,21963,4813,1773,58,9646,13718,544,9016,3231,14627,25450,19215,2408,19474,15255,2045,13608,5579,3133,1770,1368,7075,18164,9730,423,4130,19472,10120],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/leiAYcRDGTSl5B1eCnwpSGqmDEUEfDPPoYisFGhT_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/leiAYcRDGTSl5B1eCnwpSGqmDEUEfDPPoYisFGhT_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/ARM4e6URKRsbRZvIF0vFis9DjxGloBjnBYJXbHmZ_medium.webp","\/storage\/images\/resized\/LsKy6OnmmmRGcAU6CZgWQvNiP1polbaSLNrN7zqj_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/leiAYcRDGTSl5B1eCnwpSGqmDEUEfDPPoYisFGhT_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/A7oGMwx1m3fFJlKJvQUVra8h0itmEa7JZW0q3Q0b_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp"],"datasets":[{"label":"","data":[18,10,9,9,9,6,6,5,4,4,4,4,4,3,3,3,3,2,2,2,2,2,2,2,2,2,2,2,2],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[9.05,5.03,4.52,4.52,4.52,3.02,3.02,2.51,2.01,2.01,2.01,2.01,2.01,1.51,1.51,1.51,1.51,1.01,1.01,1.01,1.01,1.01,1.01,1.01,1.01,1.01,1.01,1.01,1.01],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["American Chemical Society (ACS)","Wiley","Royal Society of Chemistry (RSC)","Elsevier","Springer Nature","Multidisciplinary Digital Publishing Institute (MDPI)","Pleiades Publishing","IOP Publishing","American Institute of Physics (AIP)","Taylor & Francis","Frontiers Media S.A.","Annual Reviews","American Association for the Advancement of Science (AAAS)","American Physical Society (APS)","The Chemical Society of Japan","Pirogov Russian National Research Medical University","American Society for Cell Biology (ASCB)","Oxford University Press"],"ids":[40,11,123,17,8,202,101,2075,250,18,208,6941,189,1539,6950,5654,4151,19],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/leiAYcRDGTSl5B1eCnwpSGqmDEUEfDPPoYisFGhT_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/LsKy6OnmmmRGcAU6CZgWQvNiP1polbaSLNrN7zqj_medium.webp","\/storage\/images\/resized\/ARM4e6URKRsbRZvIF0vFis9DjxGloBjnBYJXbHmZ_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/A7oGMwx1m3fFJlKJvQUVra8h0itmEa7JZW0q3Q0b_medium.webp","\/storage\/images\/resized\/s10mcsCV4OAUg9O2KrqOquQC0PhyLMI8hUUkuflM_medium.webp","\/storage\/images\/resized\/nrK64iXHTzj43wMrfN1ZoUQ0vanswGzWPN45K3jA_medium.webp","\/storage\/images\/resized\/ktSZPrGjOMvauY6mn7jk8bqo3Af0S6abv653w90B_medium.webp","\/storage\/images\/resized\/YbblL7g7LZxen4zAlAZD3Qq9UGfDJZMh3izylBaT_medium.webp","\/storage\/images\/resized\/1pFWD0EzQnc5egizpglbyNDhAbHCAVj1O7hBchfP_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp"],"datasets":[{"label":"","data":[65,31,25,20,11,7,5,5,5,4,3,3,2,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[32.66,15.58,12.56,10.05,5.53,3.52,2.51,2.51,2.51,2.01,1.51,1.51,1.01,0.5,0.5,0.5,0.5,0.5],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}

Metrics

Cite this

GOST |

Cite this

GOST Copy

Acharya A. et al. Photoinduced chemistry in fluorescent proteins: Curse or blessing? // Chemical Reviews. 2016. Vol. 117. No. 2. pp. 758-795.

GOST all authors (up to 50) Copy

Acharya A., Bogdanov A. M., Grigorenko B., Bravaya K., Nemukhin A., Lukyanov K. A., Krylov A. I. Photoinduced chemistry in fluorescent proteins: Curse or blessing? // Chemical Reviews. 2016. Vol. 117. No. 2. pp. 758-795.

RIS |

Cite this