Journal of Physical Chemistry B, volume 119, issue 17, pages 5444-5452
A Light-Induced Reaction with Oxygen Leads to Chromophore Decomposition and Irreversible Photobleaching in GFP-Type Proteins
Publication type: Journal Article
Publication date: 2015-04-21
Journal:
Journal of Physical Chemistry B
Quartile SCImago
Q1
Quartile WOS
Q3
Impact factor: 3.3
ISSN: 15206106, 15205207
Materials Chemistry
Surfaces, Coatings and Films
Physical and Theoretical Chemistry
Abstract
Photobleaching and photostability of proteins of the green fluorescent protein (GFP) family are crucially important for practical applications of these widely used biomarkers. On the basis of simulations, we propose a mechanism for irreversible bleaching in GFP-type proteins under intense light illumination. The key feature of the mechanism is a photoinduced reaction of the chromophore with molecular oxygen (O2) inside the protein barrel leading to the chromophore's decomposition. Using quantum mechanics/molecular mechanics (QM/MM) modeling we show that a model system comprising the protein-bound Chro(-) and O2 can be excited to an electronic state of the intermolecular charge-transfer (CT) character (Chro(•)···O2(-•)). Once in the CT state, the system undergoes a series of chemical reactions with low activation barriers resulting in the cleavage of the bridging bond between the phenolic and imidazolinone rings and disintegration of the chromophore.
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Citations by publishers
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10
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Wiley
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Springer Nature
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Springer Nature
1 publication, 3.7%
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American Institute of Physics (AIP)
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American Institute of Physics (AIP)
1 publication, 3.7%
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Grigorenko B. et al. A Light-Induced Reaction with Oxygen Leads to Chromophore Decomposition and Irreversible Photobleaching in GFP-Type Proteins // Journal of Physical Chemistry B. 2015. Vol. 119. No. 17. pp. 5444-5452.
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Grigorenko B., Nemukhin A., Polyakov I. V., Khrenova M. G., Krylov A. I. A Light-Induced Reaction with Oxygen Leads to Chromophore Decomposition and Irreversible Photobleaching in GFP-Type Proteins // Journal of Physical Chemistry B. 2015. Vol. 119. No. 17. pp. 5444-5452.
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TY - JOUR
DO - 10.1021/acs.jpcb.5b02271
UR - https://doi.org/10.1021%2Facs.jpcb.5b02271
TI - A Light-Induced Reaction with Oxygen Leads to Chromophore Decomposition and Irreversible Photobleaching in GFP-Type Proteins
T2 - Journal of Physical Chemistry B
AU - Polyakov, Igor V.
AU - Grigorenko, Bella
AU - Nemukhin, Alexander
AU - Khrenova, Maria G.
AU - Krylov, Anna I.
PY - 2015
DA - 2015/04/21 00:00:00
PB - American Chemical Society (ACS)
SP - 5444-5452
IS - 17
VL - 119
SN - 1520-6106
SN - 1520-5207
ER -
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@article{2015_Grigorenko,
author = {Igor V. Polyakov and Bella Grigorenko and Alexander Nemukhin and Maria G. Khrenova and Anna I. Krylov},
title = {A Light-Induced Reaction with Oxygen Leads to Chromophore Decomposition and Irreversible Photobleaching in GFP-Type Proteins},
journal = {Journal of Physical Chemistry B},
year = {2015},
volume = {119},
publisher = {American Chemical Society (ACS)},
month = {apr},
url = {https://doi.org/10.1021%2Facs.jpcb.5b02271},
number = {17},
pages = {5444--5452},
doi = {10.1021/acs.jpcb.5b02271}
}
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MLA
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Grigorenko, Bella, et al. “A Light-Induced Reaction with Oxygen Leads to Chromophore Decomposition and Irreversible Photobleaching in GFP-Type Proteins.” Journal of Physical Chemistry B, vol. 119, no. 17, Apr. 2015, pp. 5444-5452. https://doi.org/10.1021%2Facs.jpcb.5b02271.