Head of Laboratory

Lyabin, Dmitry N

DSc in Biological/biomedical sciences
Publications
32
Citations
1 130
h-index
15
Authorization required.
Lab team

The study of the major protein of mRNP complexes YB-1 and its homologues.

In addition to studying the properties and functions of the Y-box binding protein, the group also deals with other problems of molecular biology:

- Transcriptional-translational coregulation in the mTOR-mediated translational response.

- Transcription factors in the promoter regions of mTOR mRNA targets.

- Negative selection of mutations in transcription factor binding sites in cancer.

More details: https://protres.ru/unit/gruppa-regulyacii-biosinteza-belka

  1. Isolation and purification of target proteins
  2. Isolation of nucleic acids
  3. Isolation of ribonucleoprotein complexes, work with ribosomes and their complexes
  4. Development and isolation of recombinant proteins from E.coli or insect cells (using baculovirus vectors) by affinity chromatography
  5. Circular dichroism spectroscopy
  6. PCR
  7. RNA-seq
  8. Ribosome profiling
  9. Cloning the Golden Gate
  10. Real-time PCR and digital PCR, including design and validation of primers and probes to your target
  11. Cloning
Lyabin, Dmitry N
Dmitry Lyabin
Head of Laboratory
Kulakovskiy, Ivan V V
Ivan V Kulakovskiy
Leading researcher

Research directions

The study of protein biosynthesis

+
The study of the major protein of mRNP complexes YB-1 and its homologues. Transcriptional-translational coregulation in the mTOR-mediated translational response. Transcription factors in the promoter regions of mTOR mRNA targets. Negative selection of mutations in transcription factor binding sites in cancer.

Partners

Lab address

Институтская улица, 4, Пущино, городской округ Серпухов, Московская область, 142290
Authorization required.