Starodumova I.P., Dorofeeva L.V., Prisyazhnaya N.V., Tarlachkov S.V., Vasilenko O.V., Avtukh A.N., Ospennikov Y.V., Subbotin S.A., Evtushenko L.I.
2024-03-27 citations by CoLab: 0 Abstract
Two novel yellow-pigmented, rod-shaped and non-motile coryneform actinobacteria, strains VKM Ac-2596T and VKM Ac-2761, were isolated from a plant Tanacetum vulgare (Asteraceae) infested by foliar nematode Aphelenchoides sp. The strains exhibited the highest 16S rRNA gene sequence similarities to Rathayibacter agropyri CA4T (99.71%), Rathayibacter rathayi DSM 7485T (99.65%) and Rathayibacter iranicus VKM Ac-1602T (99.65%). The pairwise average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between VKM Ac-2596T and VKM Ac-2671 towards the type strains of Rathayibacter species did not exceed 85.24% and 29.40%, respectively, that are well below the thresholds for species delineation. The target strains had key chemotaxonomic properties typical of the genus Rathayibacter, namely, the DAB-based peptidoglycan, rhamnose and mannose as the predominant sugars and a rhamnomannan in the cell, the major menaquinone MK-10 and fatty acids of iso-anteiso type, with a large proportion of anteiso-15:0. The strains showed clear differences from the recognized Rathayibacter species in several phenotypic characteristics, including the difference in the composition of cell wall glycopolymers. Based on the results obtained in this study and the data published previously, we provide a description of a new species, Rathayibacter tanaceti sp. nov., with DL-642T (= VKM Ac-2596T = LMG 33114T) as the type strain.
Potekhina N.V., Tul’skaya E.M., Ospennikov Y.V., Evtushenko L.I.
Microbiology Q3 Q4
2023-12-01 citations by CoLab: 1 Abstract
Information is provided on the cell wall glycopolymers of members of eight species of the genus Rathayibacter (family Microbacteriaceae, class Actinomycetes). The composition, structure, and structural components of polymers that are common to the genus and characteristic of species are noted, which can be useful as chemotaxonomic characters. The differences between Rathayibacter and other genera of the family Microbacteriaceae (Clavibacter, Curtobacterium) in the type and composition of glycopolymers are shown.
Tarlachkov S.V., Ospennikov Y.V., Demidov A.V., Starodumova I.P., Dorofeeva L.V., Prisyazhnaya N.V., Chizhov V.N., Subbotin S.A., Evtushenko L.I.
2022-10-20 citations by CoLab: 1 Abstract
Draft genome sequences of 9 strains of known and putative new species of Microbacteriaceae isolated from insect- and nematode-damaged plants were generated using Illumina technology. The data obtained will contribute to the development of the genome-based prokaryote taxonomy and the knowledge on the biology of the microbial group investigated.
Starodumova I.P., Dorofeeva L.V., Prisyazhnaya N.V., Tarlachkov S.V., Vasilenko O.V., Avtukh A.N., Ospennikov Y.V., Subbotin S.A., Evtushenko L.I.
2024-03-27 citations by CoLab: 0 Abstract
Two novel yellow-pigmented, rod-shaped and non-motile coryneform actinobacteria, strains VKM Ac-2596T and VKM Ac-2761, were isolated from a plant Tanacetum vulgare (Asteraceae) infested by foliar nematode Aphelenchoides sp. The strains exhibited the highest 16S rRNA gene sequence similarities to Rathayibacter agropyri CA4T (99.71%), Rathayibacter rathayi DSM 7485T (99.65%) and Rathayibacter iranicus VKM Ac-1602T (99.65%). The pairwise average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between VKM Ac-2596T and VKM Ac-2671 towards the type strains of Rathayibacter species did not exceed 85.24% and 29.40%, respectively, that are well below the thresholds for species delineation. The target strains had key chemotaxonomic properties typical of the genus Rathayibacter, namely, the DAB-based peptidoglycan, rhamnose and mannose as the predominant sugars and a rhamnomannan in the cell, the major menaquinone MK-10 and fatty acids of iso-anteiso type, with a large proportion of anteiso-15:0. The strains showed clear differences from the recognized Rathayibacter species in several phenotypic characteristics, including the difference in the composition of cell wall glycopolymers. Based on the results obtained in this study and the data published previously, we provide a description of a new species, Rathayibacter tanaceti sp. nov., with DL-642T (= VKM Ac-2596T = LMG 33114T) as the type strain.
Kirilova M., Yotinov I., Todorova Y., Dinova N., Lincheva S., Schneider I., Topalova Y.
Processes Q2 Q2 Open Access
2024-01-09 citations by CoLab: 2 PDF Abstract
During adaptation to waters that are rich in xenobiotics, biological systems pass through multiple stages. The first one is related to the restructuring of communities, pronounced destruction of the structure, and multiplication of active biodegradants. The purpose of the present research was to describe the microbiome restructuring that occurs during the adaptation stage in landfill leachate treatment. In a model SBR (sequencing batch reactor), a 21-day purification process of landfill leachate was simulated. Wastewater was fed in increasing concentrations. When undiluted leachate entered, the activated sludge structure disintegrated (Sludge Volume Index—4.6 mL/g). The Chemical Oxygen Demand and ammonium nitrogen concentration remained at high values in the influent (2321.11 mgO2/L and 573.20 mg/L, respectively). A significant amount of free-swimming cells was found, and the number of aerobic heterotrophs and bacteria of the genera Pseudomonas and Acinetobacter increased by up to 125 times. The Azoarcus-Thauera cluster (27%) and Pseudomonas spp. (16%) were registered as the main bacterial groups in the activated sludge. In the changed structure of the microbial community, Gammaproteobacteria, family Rhizobiaceae, class Saccharimonadia were predominantly represented. Among the suspended bacteria, Microbactericeae and Burkholderiaceae, which are known for their ability to degrade xenobiotics, were present in larger quantities. The enzymological analysis demonstrated that the ortho-pathway of cleavage of aromatic structures was active in the community. The described changes in the leachate-purifying microbial community appear to be destructive at the technological level. At the microbiological level, however, trends of initial adaptation were clearly outlined, which, if continued, could provide a highly efficient biodegradation community.
Potekhina N.V., Tul’skaya E.M., Ospennikov Y.V., Evtushenko L.I.
Microbiology Q3 Q4
2023-12-01 citations by CoLab: 1 Abstract
Information is provided on the cell wall glycopolymers of members of eight species of the genus Rathayibacter (family Microbacteriaceae, class Actinomycetes). The composition, structure, and structural components of polymers that are common to the genus and characteristic of species are noted, which can be useful as chemotaxonomic characters. The differences between Rathayibacter and other genera of the family Microbacteriaceae (Clavibacter, Curtobacterium) in the type and composition of glycopolymers are shown.
Stoll D.A., Grimmler C., Hetzer B., Kulling S.E., Huch M.
The novel, aerobic, Gram-stain-positive, rod-shaped bacterial strain, ZW T2_19T, was isolated from an onion sample (Allium cepa var. Rijnsburger). Analyses of the 16S rRNA gene sequence revealed that ZW T2_19T represented a member of the genus Rathayibacter but may represent a novel species of this genus. Analyses of the whole draft genome sequences, i.e. digital DNA–DNA hybridisation (dDDH) and average nucleotide identity (ANI) of ZW T2_19T and all type strains of species of the genus Rathayibacter confirmed that ZW T2_19T represents a novel species of the genus Rathayibacter . The genome size of ZW T2_19T is 4.01 Mbp and the DNA G+C content is 71.8 mol%. Glucose, mannose, rhamnose and ribose were detected as whole-cell sugars of ZW T2_19T. The major respiratory quinone of ZW T2_19T is menaquinone MK-10, at 78.9 %. The detected peptidoglycan type in ZW T2_19T is a variant of type B2γ with {Gly} [l-diaminobutyric acid (l-DAB)/l-homoserine (l-Hse)] d-Glu-l-DAB. Polar lipids in ZW T2_19T consisted of one diphosphatidylglycerol, one phosphatidylglycerol, seven glycolipids, one phospholipid and one lipid. The fatty acid profile of ZW T2_19T predominantly consisted of anteiso-C15 : 0 (53 %), iso-C16 : 0 (21 %) and anteiso-C17 : 0 (18 %). In addition, API 20NE, API 50CH, API Coryne, API ZYM, antibiotic susceptibility, haemolysis and growth at different temperatures and with different supplements was investigated. On the basis of the results obtained using this polyphasic approach, including molecular, phenotypic and biochemical analyses, we propose the novel species Rathayibacter rubneri with the type and only strain ZW T2_19T (= DSM 114294T = LMG 32700T).
Tarasov K., Yakhnenko A., Zarubin M., Gangapshev A., Potekhina N.V., Avtukh A.N., Kravchenko E.
2022-12-07 citations by CoLab: 7 Abstract
In this study, we present the characterization of the BNO1T bacterial strain isolated from the deep subsurface saline spring at the Baksan Neutrino Observatory INR RAS (Kabardino-Balkaria, Russia). The complete genome sequence of the strain BNO1T is 5,347,902 bp, with a GC content 41 and 49%. The cell wall peptidoglycan contains meso-diaminopimelic acid. The major isoprenoid quinone is MK-7 and the polar lipids are diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The major fatty acids are anteiso-C15:0 (23.34%), iso-C15:0 (20.10%), C16:0 (11.96%), iso-C16:0 (10.88%), and anteiso-C17:0 (10.79%). The 16S rRNA gene sequence clearly demarcated the strain as belonging to Cytobacillus genera. Based on the phylogenetic analysis, ANI (average nucleotide identity) and dDDH (digital DNA-DNA hybridization) assessments we propose to assign the strain BNO1T and other related strains to new species and to name it Cytobacillus pseudoceanisediminis sp. nov. (The values of ANI and dDDH between BNO1T and Cytobacillus oceanisediminis CGMCC 1.10115 T are 80.65% and 24.7%, respectively; values of ANI and dDDH between BNO1T and Cytobacillus firmus NCTC 10335 T are 89% and 38%, respectively). Genomic analysis of strain BNO1T revealed pathways for C1 compounds oxidation and two pathways for C1 compounds assimilation: serine and ribulose monophosphate pathways. In addition, strain BNO1T contains a plasmid (342,541 bp) coding multiple genes involved in heavy metal ion balance. Moreover, heavy metal toxicity testing confirmed the high potential of the strain BNO1T as a source of metal resistance genes and enzymes. The type strain is BNO1T (= BIM B-1921 T = VKM B-3664 T).
Guérin H., Kulakauskas S., Chapot-Chartier M.
2022-10-01 citations by CoLab: 25 Abstract
Rhamnose-rich cell wall polysaccharides (Rha-CWPSs) have emerged as crucial cell wall components of numerous Gram-positive, ovoid-shaped bacteria-including streptococci, enterococci, and lactococci-of which many are of clinical or biotechnological importance. Rha-CWPS are composed of a conserved polyrhamnose backbone with side-chain substituents of variable size and structure. Because these substituents contain phosphate groups, Rha-CWPS can also be classified as polyanionic glycopolymers, similar to wall teichoic acids, of which they appear to be functional homologs. Recent advances have highlighted the critical role of these side-chain substituents in bacterial cell growth and division, as well as in specific interactions between bacteria and infecting bacteriophages or eukaryotic hosts. Here, we review the current state of knowledge on the structure and biosynthesis of Rha-CWPS in several ovoid-shaped bacterial species. We emphasize the role played by multicomponent transmembrane glycosylation systems in the addition of side-chain substituents of various sizes as extracytoplasmic modifications of the polyrhamnose backbone. We provide an overview of the contribution of Rha-CWPS to cell wall architecture and biogenesis and discuss current hypotheses regarding their importance in the cell division process. Finally, we sum up the critical roles that Rha-CWPS can play as bacteriophage receptors or in escaping host defenses, roles that are mediated mainly through their side-chain substituents. From an applied perspective, increased knowledge of Rha-CWPS can lead to advancements in strategies for preventing phage infection of lactococci and streptococci in food fermentation and for combating pathogenic streptococci and enterococci.
2022-01-01 citations by CoLab: 15 Abstract
Summary: The TaxonDC program (Taxon Distance Calculator) performs pairwise sequence alignment followed by determining the similarity value between two or more sequences of interest. Unlike widely used programs, TaxonDC makes only pairwise alignment of input sequences that allows avoiding different similarity values depending on the sequences included in the analysis. The similarity values calculated with TaxonDC are the same compared to those calculated using popular identification oriented web-based tool EzBioCloud that makes calculated values comparable with previous ones. In addition, to help prevent discrepancy among different researchers, the problem concerning the influence of the order of entered sequences on similarity values is specially considered. To our knowledge, TaxonDC is the only software which includes these capabilities in combination, simplifies and widens calculation of similarity values in systematics of prokaryotes and eukaryotes. The program has easy-to-use interface and can be run on Windows and Linux. Availability and Implementation: The program is available free of charge at https://tarlachkov.ru/en/software/taxondc. Supplementary information: Supplementary data are available at Journal of Bioinformatics and Genomics online.
Vandamme P., Sutcliffe I.
Chemotaxonomic methods played an important role in the development of the polyphasic approach to classification of Archaea and Bacteria. However, we here argue that routine application of these methods is unnecessary in an era when genomic data are available and sufficient for species delineation. Thus, authors who choose not to utilize such methods should not be forced to do so during the peer review and editorial handling of manuscripts describing novel species. Instead, we argue that chemotaxonomy will thrive if improved analytical methods are introduced and deployed, primarily by specialist laboratories, in studies at taxonomic levels above the characterisation of novel species.
Tancos M.A., McMahon M.B., Garrett W.M., Luster D.G., Rogers E.E.
2021-09-01 citations by CoLab: 2 Abstract
Phytopathogenic Rathayibacter species are unique bacterial plant pathogens because they are obligately vectored by plant parasitic anguinid nematodes to the developing seedheads of forage grasses and cereals. This understudied group of plant-associated Actinomycetes includes the neurotoxigenic plant pathogen R. toxicus, which causes annual ryegrass toxicity in grazing livestock. R. toxicus is currently endemic to Australia and is listed as a plant pathogen select agent by the U.S. Department of Agriculture-Animal and Plant Health Inspection Service. The complex Rathayibacter disease cycle requires intimate interactions with the nematode vector and plant hosts, which warrants an increased understanding of the secretory and surface-associated proteins that mediate these diverse eukaryotic interactions. Here we present the first comparative secretome analysis for this complex, nematode-vectored Rathayibacter genus that compares the three agronomically damaging toxigenic and atoxigenic Rathayibacter species, R. toxicus, R. iranicus, and R. tritici. The exoproteomic comparison identified 1,423 unique proteins between the three species via liquid chromatography-tandem mass spectrometry, leading to the identification of putative pathogenicity-related proteins and proteins that may mediate nematode attachment. Of the uniquely identified proteins, 94 homologous proteins were conserved between the three Rathayibacter exoproteomes and comprised between 43.4 and 58.6% of total protein abundance. Comparative analyses revealed both conserved and uniquely expressed extracellular proteins, which, interestingly, had more similarities to extracellular proteins commonly associated with bacterial animal pathogens than classic plant pathogens. This comparative exoproteome analysis will facilitate the characterization of proteins essential for vector attachment and host colonization and assist in the development of serological diagnostic assays.
Zaychikov V.A., Potekhina N.V., Dmitrenok A.S., Fan D., Tul’skaya E.M., Dorofeeva L.V., Evtushenko L.I.
Microbiology Q3 Q4
2021-05-01 citations by CoLab: 6 Abstract
Presence of the cell wall glycopolymer rhamnan was established for members of the genus Curtobacterium (family Microbacteriaceae) by using chemical and NMR spectroscopic methods. The rhamnan with the repeating unit, →2)-α-L-Rhap-(1→3)-α-L-Rhap-(1→, was found in C. flaccumfaciens pv. flaccumfaciens VKM Ас-1923T, C. flaccumfaciens pv. poinsettiae VKM Ас-1924T, C. pusillum VKM Ac-2099T, C. herbarum VKM Ac-2512T, and Curtobacterium sp. VKM Ac-1811. Each of the studied strains also contained a second, acidic phosphate-free polysaccharide. NMR spectra of these polysaccharides differed, indicating the structural differences. Cell wall hydrolysates of all studied strains were found to contain rhamnose, mannose, glucose, galactose, and glucosamine. Further research will facilitate more detailed characterization of the composition of cell wall glycopolymers in Curtobacterium species, as well as evaluation of their taxonomic significance. The data on the polymer structure will contribute to understanding of the mechanisms of colonization and infection of higher organisms by Curtobacterium species and bacterial interaction in microbial communities and with abiotic environmental objects.
Shashkov A.S., Tul’skaya E.M., Potekhina N.V., Dmitrenok A.S., Senchenkova S.N., Zaychikov V.A., Dorofeeva L.V., Evtushenko L.I.
2021-04-09 citations by CoLab: 5 Abstract
Rathayibacter sp. VKM Ac-2759 (family Microbacteriaceae, class Actinobacteria) contains two glycopolymers in the cell wall. The main chain of rhamnan, glycopolymer 1, is built from the repeating tetrasaccharide units carrying terminal arabinofuranose residues at the non-reducing end, →3)-α-[α-D-Araf-(1→2)]-D-Rhap-(1→2)-α-D-Rhap-(1→3)-α-D-Rhap-(1→2)-α-D-Rhap-(1→. Similar to other described Rathayibacter species, rhamnose in the neutral glycopolymer of the VKM Ac-2759 strain is present in the D-configuration. Acetalated with pyruvic acid teichuronic acid, glycopolymer 2, is composed of the repeating tetrasaccharide units, →4)-β-D-GlcpA-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→3)-β-[4,6-S-Pyr]-D-Manp-(1→. Glycopolymers 1 and 2 were identified in prokaryotic microorganisms for the first time and their structures were established by chemical analysis and NMR spectroscopy. The obtained data can be used in taxonomic research, as well as for elucidating the mechanisms of plant colonization and infection by bacteria of the Rathayibacter genus.
Tarlachkov S.V., Starodumova I.P., Dorofeeva L.V., Prisyazhnaya N.V., Roubtsova T.V., Chizhov V.N., Nadler S.A., Subbotin S.A., Evtushenko L.I.
2021-03-04 citations by CoLab: 5 Abstract
Draft genome sequences of 28 strains of Microbacteriaceae from plants infested by plant-parasitic nematodes were obtained using Illumina technology. The sequence data will provide useful baseline information for the development of comparative genomics and systematics of Microbacteriaceae and facilitate understanding of molecular mechanisms involved in interactions between plants and nematode-associated bacterial complexes. ABSTRACT Draft genome sequences of 28 strains of Microbacteriaceae from plants infested by plant-parasitic nematodes were obtained using Illumina technology. The sequence data will provide useful baseline information for the development of comparative genomics and systematics of Microbacteriaceae and facilitate understanding of molecular mechanisms involved in interactions between plants and nematode-associated bacterial complexes.
Shashkov A.S., Potekhina N.V., Tul'skaya E.M., Dmitrenok A.S., Senchenkova S.N., Dorofeeva L.V., Zaychikov V.A., Evtushenko L.I.
2021-01-10 citations by CoLab: 7 Abstract
The cell wall of Rathayibacter cari cis VKM Ac-1799 T (family Microbacteriaceae , class Actinobacteria ) was found to contain both neutral and acidic glycopolymers. The first one is D-rhamnopyranan with main chain →2)-α-D-Rha p -(1 → 3)-α-D-Rha p -(1→, where a part of 2-substituted residues bears as a side-chain at position 3 α-D-Man p residues or disaccharides α-D-Ara f -(1→2)-α-D-Man p -(1 → . The second polymer is a teichuronic acid with a branched repeating units composed of seven monosaccharides →4)-α-[β-D-Man p -(1 → 3)]-D-Glc p -(1 → 4)-β-D-Glc p A-(1 → 2)-β-[4,6Pyr]-D-Man p -(1 → 4)-β-L-Rha p -(1 → 4)-β-D-Glc p -(1 → 4)-β-D-Glc p -(1 → . The structures of the polymers were determined by chemical and NMR spectroscopic methods. • The D-rhamnan and teichuronic acid were isolated from Rathayibacter cariscis VKM Ac-1799 T cell wall. • The structures of the polymers were determined by chemical and NMR spectroscopic methods. • D-rhamnan is composed of linear and branched repeating units. • The teichuronic acid consists of pyruvilated branched heptosaccharide units.
Criscuolo A.
2020-11-10 citations by CoLab: 24 Abstract
Recently developed MinHash-based techniques were proven successful in quickly estimating the level of similarity between large nucleotide sequences. This article discusses their usage and limitations in practice to approximating uncorrected distances between genomes, and transforming these pairwise dissimilarities into proper evolutionary distances. It is notably shown that complex distance measures can be easily approximated using simple transformation formulae based on few parameters. MinHash-based techniques can therefore be very useful for implementing fast yet accurate alignment-free phylogenetic reconstruction procedures from large sets of genomes. This last point of view is assessed with a simulation study using a dedicated bioinformatics tool.
Tarlachkov S.V., Starodumova I.P., Dorofeeva L.V., Prisyazhnaya N.V., Leyn S.A., Zlamal J.E., Elane M.L., Osterman A.L., Nadler S.A., Subbotin S.A., Evtushenko L.I.
2020-05-28 citations by CoLab: 9 Abstract
Complete and draft genome sequences of 12 Rathayibacter strains were generated using Oxford Nanopore and Illumina technologies. The genome sizes of these strains are 3.21 to 4.61 Mb, with high G+C content (67.2% to 72.7%) genomic DNA. Genomic data will provide useful baseline information for natural taxonomy and comparative genomics of members of the genus Rathayibacter. ABSTRACT Complete and draft genome sequences of 12 Rathayibacter strains were generated using Oxford Nanopore and Illumina technologies. The genome sizes of these strains are 3.21 to 4.61 Mb, with high G+C content (67.2% to 72.7%) genomic DNA. Genomic data will provide useful baseline information for natural taxonomy and comparative genomics of members of the genus Rathayibacter.
Shashkov A.S., Tul’skaya E.M., Streshinskaya G.M., Dmitrenok A.S., Potekhina N.V., Senchenkova S.N., Piskunkova N.F., Dorofeeva L.V., Evtushenko L.I.
2020-03-22 citations by CoLab: 11 Abstract
The structures of three cell wall glycopolymers of the phytopathogen Rathayibacter tritici VKM Ac-1603T (family Microbacteriaceae, order Micrococcales, class Actinobacteria) were established by chemical methods and NMR spectroscopy. Polymer 1 is a branched rhamnomannan with the repeating unit →3)-α-[β-D-Xylp-(1→2)]-D-Manp-(1→2)-α-D-Rhap-(1→3)-α-D-Manp-(1→2)-α-D-Rhap-(1→; polymer 2 is a linear rhamnomannan with the repeating unit →2)-α-D-Manp-(1→2)-α-D-Rhap-(1→3)-α-D-Manp-(1→2)-α-D-Rhap-(1→; polymer 3 is a branched teichuronic acid containing monosaccharide residues GlcA, Gal, Man, and Glc at a 1:1:1:5 ratio (see the text for the structures). It has been demonstrated that representatives of four Rathayibacter species studied to date (R. tritici VKM Ac-1603T, R. iranicus VKM Ac-1602T, R. toxicus VKM Ac-1600 and “Rathayibacter tanaceti” VKM Ac-2596) contain differing patterns of phosphate-free glycopolymers. At the same time, the above Rathayibacter strains have a common property — the presence of rhamnomannans with D-rhamnose. These rhamnomannans may be linear or branched and differing in the positions of glycosidic bonds and side substituents. The presence in the cell wall of rhamnomannans with D-rhamnose may serve as useful chemotaxonomic marker of the genus Rathayibacter.
Dmitrenok A.S., Shashkov A.S., Streshinskaya G.M., Tul'skaya E.M., Potekhina N.V., Senchenkova S.N., Dorofeeva L.V., Evtushenko L.I.
2019-08-01 citations by CoLab: 9 Abstract
The cell wall of Rathayibacter iranicus VKM Ac-1602 T (family Microbacteriaceae, class Actinobacteria) is characterised by the absence of phosphate-containing and by the presence of two rhamnose-containing glycopolymers. The first is a branched rhamnomannan, in which 60% of mannose residues of the main chain are glycosylated by terminal mannose residues: →2)-α-D-Rha p -(1 → 3)-α-[α-D-Man p -(1 → 6)]-D-Man p -(1 → . The second is a branched teichuronic acid, in which all the rhamnose residues of the main chain are glycosylated by glucose residues:→3)-α-[α-D-Glc p -(1 → 2)]-L-Rha p -(1 → 4)-β-D-Glc p A-(1 → 2)-α-D-Man p -(1 → 3)-α-D-Gal p -(1 → 3)-β-D-Glc p -(1 → . Both glycopolymers have the unique structures and described in the cell walls of Gram-positive bacteria for the first time. The obtained data allow for a more complete characterisation of the cell wall of the microorganism under investigation and can serve as a phenotypic characterisation of this bacterium. The glycopolymer structures were established using chemical and nuclear magnetic resonance (NMR) spectroscopy methods. • The structures of two branched cell wall rhamnose-containing polymers are identified. • The neutral rhamnomannan contains D-Rha p , D-Man p in the ratio 2:3. • The acidic contains L-Rha p , D-Glc p A, D-Man p , D-Gal p, D-Glc p in the ratio 1:1:1:1:2.
Total publications
3
Total citations
2
Citations per publication
0.67
Average publications per year
1
Average coauthors
6.33
Publications years
2022-2024 (3 years)
h-index
1
i10-index
0
m-index
0.33
o-index
1
g-index
1
w-index
0
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Top-100

Fields of science

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2
Microbiology, 2, 66.67%
Applied Microbiology and Biotechnology, 2, 66.67%
Molecular Biology, 1, 33.33%
General Medicine, 1, 33.33%
Genetics, 1, 33.33%
Immunology and Microbiology (miscellaneous), 1, 33.33%
1
2

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1
1

Citing journals

1
1

Publishers

1
1

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1
2
3
Russia, 3, 60%
USA, 2, 40%
1
2
3

Citing organizations

1
Organization not defined, 1, 25%
1

Citing countries

1
Russia, 1, 33.33%
USA, 1, 33.33%
Bulgaria, 1, 33.33%
1
  • We do not take into account publications without a DOI.
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Position
Engineer
Employment type
Part time
Years
2021 — present