Pozdnyakova-Filatova, Irina Yu
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Publications
9
Citations
29
h-index
4
Education
Tula State University
2007 — 2012,
Specialist, Natural Science
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Petrikov K.V., Vetrova A.A., Ivanova A.A., Sazonova O.I., Pozdnyakova-Filatova I.Y.
The elimination of hydrocarbon pollution is a critical environmental challenge, often addressed using biotechnological approaches. The study of alkane oxidizing bacteria is an important task, as it will allow developing new approaches to oil pollution treatment and improving existing ones. Representatives of the genus Rhodococcus are well-known hydrocarbon-oxidizing bacteria with high biotechnological potential. A distinctive feature of some Rhodococcus species is the presence of multiple homologous alkB genes within their genomes. However, the reasons behind this redundancy and the functional diversity of the alkane monooxygenases encoded by these genes remain unclear. Furthermore, despite the significant diversity of alkB genes even within this single genus, a comprehensive classification system has yet to be established. In this study, we conducted a phylogenetic analysis of all AlkB family alkane monooxygenases identified in Rhodococcus genomes and examined the genomic context of their corresponding genes. Our findings reveal a remarkable diversity of AlkB enzymes, refine the classification of previously described AlkB types, and propose three novel types.For the AlkB0 type, we identified deviations from previously reported signature amino acid motifs. Our results highlight the key role of colocalization of alkane monooxygenase alkB genes with rubredoxin rubB genes in the further functional classification of alkane monooxygenases.
Pozdnyakova-Filatova I.Y., Ivanova E.V., Petrikov K.V., Rezhepova A., Frolova A.A., Zakharova M.V.
The residual content of the only carbon source increases at the end of the exponential growth phase when P. putida BS3701 cells are cultured under nitrogen deficiency. Moreover, the mRNA quantity of some genes involved in carbon catabolism decreases. There are no available data on regulators capable of stimulating the target mRNA degradation in pseudomonads under nitrogen deficiency in the literature. We have applied both comparative genomics methods and a number of biomolecular ones to identify new small P. putida BS3701 RNAs. This is due to the fact that the formation of ncRNA-mRNA duplexes, recognized by RNases, is one of the main mechanisms for reducing the number of transcripts in a cell. We have used the following selection criteria: 1) the presence of the interest gene of the NtrC binding site and a sigma54-dependent promoter in the upstream area, 2) the conservative location of the interest gene in the P. putida and P. aeruginosa genomes, 3) increase in the product quantity of the interest gene in a cell under nitrogen deficiency on two different carbon sources (succinate and naphthalene), 4) the product detection of the interest gene in a fraction enriched with small RNAs. The expression of two new small RNAs has increased twice or more times under nitrogen deficiency on both succinate and naphthalene: expression of ng171 and ng379. ng379 contains narK RNA motif.
Pozdnyakova-Filatova I.Y., Zagoskin A.A., Zakharova M.V., Nagornykh M.O.
Objective.
To determine whether the genes whose products are annotated as «MBL-fold metallohydrolase superfamily» are related to the proteins of the metallo-β-lactamase group.
Materials and Methods.
Sequences of the 7 genes annotated as «MBL-fold metallohydrolase superfamily» were analyzed using the following resources: ClustalW, IQ-TREE, iTOL. Selection of the oligonucleotides for real-time PCR was performed using the Primer-BLAST resource. The level of gene expression was assessed using real-time PCR. MIC and MBC measuring was performed using cefepime and meropenem. The double-disc method with EDTA was used to determine the presence of MBL in the strain.
Results.
Analysis of the nucleotide sequences of the studied genes revealed that all of them were not included in the clade containing sequences of metallo-β-lactamase. In the exponential growth phase, mRNAs corresponding to the studied proteins were found. Determination of MIC and MBC revealed a low level of resistance to antibiotics of the β-lactamase group. The phenotypic test was negative for MBL in P. putida strain BS3701.
Conclusions.
The investigated genes and corresponding proteins are not related to metallo-β-lactamases. They are not involved in the resistance of P. putida BS3701 to antibiotics of the metallo-β-lactamase group.
Lobastova T., Fokina V., Pozdnyakova-Filatova I., Tarlachkov S., Shutov A., Donova M.
Steroids are abundant molecules in nature, and various microorganisms evolved to utilize steroids. Thermophilic actinobacteria play an important role in such processes. However, very few thermophiles have so far been reported capable of degrading or modifying natural sterols. Recently, genes putatively involved in the sterol catabolic pathway have been revealed in the moderately thermophilic actinobacterium Saccharopolyspora hirsuta VKM Ac-666T, but peculiarities of strain activity toward sterols are still poorly understood. S. hirsuta catalyzed cholesterol bioconversion at a rate significantly inferior to that observed for mesophilic actinobacteria (mycobacteria and rhodococci). Several genes related to different stages of steroid catabolism increased their expression in response to cholesterol as was shown by transcriptomic studies and verified by RT–qPCR. Sequential activation of genes related to the initial step of cholesterol side chain oxidation (cyp125) and later steps of steroid core degradation (kstD3, kshA, ipdF, and fadE30) was demonstrated for the first time. The activation correlates with a low cholesterol conversion rate and intermediate accumulation by the strain. The transcriptomic analyses revealed that the genes involved in sterol catabolism are linked functionally, but not transcriptionally. The results contribute to the knowledge on steroid catabolism in thermophilic actinobacteria and could be used at the engineering of microbial catalysts.
Sliadovskii D., Ponomareva T., Molchanov M., Pozdnyakova-Filatova I., Timchenko M., Marchenkov V., Gusev O., Sogorin E.
AbstractCrustacean hyaluronidases are poorly understood both in terms of their enzymatic properties and in terms of their structural features. In this work, we show that the hepatopancreas homogenate of the red king crab has a hyaluronidase activity that is an order of magnitude higher than its commercial counterpart. Zymography revealed that the molecular weight of a protein with hyalorunidase activity is 40–50 kDa. Analysis of the hepatopancreas transcriptome and results of cloning and sequencing of cDNA revealed a hyaluronidase sequence with an expected molecular weight of 42.5 kDa. Further analysis showed that hyaluronat enzymatic cleavage follows the $$\beta $$
β
-elimination mechanism, which is well known for bacterial hyaluronidases. The results of ion-exchange chromatography showed that the final product of hyaluronate degradation is unsaturated tetrasaccharide. Thus, we identified a new hyaluronidase of higher eukaryotes, which is not integrated into the modern classification of hyaluronidases.
Pozdnyakova-Filatova I., Petrikov K., Vetrova A., Frolova A., Streletskii R., Zakharova M.
Pseudomonas microorganisms are used for bioremediation of soils contaminated with petroleum hydrocarbons. The overall remediation efficiency is largely dependent on the presence of macro- and micronutrients. Widely varying concentrations of available nitrogen and iron (Fe) in soils were shown to affect residual hydrocarbons in the course of biodegradation. The regulatory mechanisms of expression of hydrocarbon catabolic genes in low nitrogen/low iron conditions remain unclear. The catabolism of naphthalene, a two-ring polycyclic aromatic hydrocarbon, has been well studied in pseudomonads in terms of the involvement of specific transcriptional activators, thus making it useful in revealing additional regulatory control of the adaptation of hydrocarbon destructors to a low level of the essential nutrients. The Pseudomonas putida strain BS3701 is a component of the “MicroBak” preparation for soil remediation. Previously, this strain was shown to contain genes encoding the key enzymes for naphthalene catabolism: naphthalene 1,2-dioxygenase, salicylate hydroxylase, catechol 2,3-dioxygenase, and catechol 1,2-dioxygenase. Our study aimed to clarify whether the naphthalene catabolic gene expression is dependent on the amount of nitrogen and iron in the growth culture medium, and if so, at exactly which stages the expression is regulated. We cultivated the strain in low nitrogen/low iron conditions with the concurrent evaluation of the activity of the key enzymes and the mRNA level of genes encoding these enzymes. We are the first to report that naphthalene catabolic genes are subject not only to transcriptional but also post-transcriptional regulation.
Skulkina K., Vetrova A., Petrikov K., Filatova I.P.
Vetoshkina D.V., Pozdnyakova-Filatova I.Y., Zhurikova E.M., Frolova A.A., Naydov I.A., Ivanov B.N., Borisova-Mubarakshina M.M.
The effect of the colonization of barley plants (Hordeum vulgare L.) by the associative microorganisms Pseudomonas putida BS3701 on the adaptation of the photosynthetic apparatus to high light intensity was studied. A number of differences were found in the functioning and structure of the photosynthetic apparatus of the control plants and plants colonized by P. putida BS3701 grown at 100 µmol quanta/m2 s. The barley plants colonized by P. putida BS3701 were shown to have an increased resistance to high light intensity (1000 µmol quanta/m2 s) when compared to the control plants. It was established that the adaptive decrease in the PSII antenna size was characterized by the same molecular mechanism in both the control plants and plants colonized by P. putida ВS3701. However, the decrease in PSII antenna size was more pronounced in plants colonized by P. putida ВS3701. It can be assumed that this might be the cause of more efficient protection of the photosynthetic apparatus of plants colonized by P. putida BS3701 against photoinhibition under high illumination.
Filatova I.Y., Kazakov A.S., Muzafarov E.N., Zakharova M.V.
Abstract Pseudomonas putida strain AK5 was the first characterized natural strain containing the ‘classical’ nah1 operon and nahR gene along with genes whose products are responsible for the less explored pathway of salicylate degradation through gentisate (the sgp operon). The sgp operon was found to be preceded by the divergently directed sgpR gene. The amino acid sequence of the sgpR product qualifies it as a LysR‐type transcriptional regulator (LTTR) and suggests its potential function as an sgp operon transcriptional regulator. This study focused on verification of SgpR's involvement in regulation of transcription of the operon genes and characterization of its interaction with the sgp promoter. We determined the transcription start for sgpAIKGHB and identified the SgpR binding site. The equilibrium dissociation constant (KD) of the SgpR‐DNA complex determined in the presence and absence of the inducer salicylate appeared to be, on the whole, at the lower end of the range for KD values reported for LTTRs. RT‐qPCR showed that in the presence of salicylate, efficiency of transcription of the sgpAIKGHB operon increased by three orders of magnitude and reached the highest values so far observed for LTTR‐controlled operons, thus holding much promise for further studies of the mechanism of transcriptional regulation that involves SgpR.
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Spiridonov V., Lukmanova A., Pozdyshev D., Antonova Y., Kusaja V., Muronetz V., Yaroslavov A.
Шелякин М.А., Силина Е.В., Головко К.Т.
Maslova S.P., Shelyakin M.A., Silina E.V., Malyshev R.V., Dalke I.V.
Data on changes in energy status, activity of pro-/antioxidant metabolism during overwintering of Heracleum sosnowskyi vegetative buds were obtained. The buds of H. sosnowskyi are not endodormant and their growth is limited by the decrease of the soil temperature to negative values at the end of November. The optimum temperature for energy storage in autumn was found to be in the range of low positive temperatures (2–5°C). The autumn buds were characterized a high capacity for the cytochrome respiratory pathway (CP, Vcyt), which accounted for 78% of the total respiration. During autumn morphogenesis and winter dormancy of buds, the levels of pro-oxidants, which are the content of thiobarbituric acid reactive substances (TBARS) and H2O2 content, remained stable. In December, when a stable snow cover and negative soil temperatures were established, the dormant buds showed 2.5 times lower rate of energy storage and activated alternative respiration capacity (AP, Valt), as indicated by a 2 times lower Vcyt/Valt ratio. In early spring, compared to winter dormancy, H2O2 levels increased 2-fold and antioxidant enzymes activity increased by 27–78% as insolation increased. Spring buds showed an increase in rate of heat production and a decrease in rate of energy storage, which may be due to spring stress caused by increased insolation. It was concluded that in the tissues of H. sosnowskyi buds subjected to exogenous dormancy, an energy balance between dormancy and growth processes is achieved at the level of respiratory capacity and pro-/antioxidant metabolism.
Kim J., Park C.S., Kang M., Moon C., Kim M., Yang S., Jang L., Jang J.Y., Jeong C.M., Lee H.S., Kim H.H.
Bovine testicular hyaluronidase (BTH), which accelerates the absorption and dispersion of drugs by decomposing hyaluronan in subcutaneous tissues, has been used in medical applications, including local anesthesia, ophthalmology, and dermatosurgery. The requirement of N-glycans for the activity of human hyaluronidase has been reported, and BTH has greater activity than human hyaluronidase. However, the N-glycan characteristics of BTH are unclear. From a commercial BTH source containing additional proteins, purified BTH (pBTH) was obtained using size exclusion chromatography, and the structures and quantities of its N-glycans were analyzed using liquid chromatography (LC)-electrospray ionization-higher energy collisional dissociation (HCD)-tandem mass spectrometry (MS/MS). In pBTH, 32 N-glycans were identified, with 12 sialylations (39.0% of total N-glycan content), nine core-fucosylations (31.5%), six terminal galactosylations (14.6%), five high-mannosylations (13.7%), and four bisecting N-acetylglucosamine structures (7.8%). The presence of sialylated glycopeptides in pBTH was confirmed by nano-LC-HCD-MS/MS analysis. The absolute quantity of all N-glycans was calculated as 1.4 pmol (0.6 pmol for sialylation) in pBTH (1.0 pmol). The sialylation level (related to half-life, thermal stability, resistance to proteolysis, and solubility) was 24.4 times higher than that of human hyaluronidase. The hyaluronan degradation activity of de-sialylated pBTH decreased to 41.2 ± 4.2%, showing that sialylated N-glycans were required for pBTH activity as well. This is the first study to identify and quantify 32 N-glycans of pBTH and investigate their structural roles in its activity. The presence of larger amounts of sialylated N-glycans in pBTH than in human hyaluronidase suggests a greater utilization of pBTH.
Gupta S.
A wide range of organic compounds, metals, and other substances which are emitted in our environment in very small concentrations are categorized as micropollutants. These substances are used by all of us in our everyday lives, while some are emitted into the environment in greater quantities as a result of large-scale industrial processes. Microplastics, pharmaceuticals, pesticides, polychlorinated biphenyls (PCBs), and polycyclic aromatic hydrocarbons (PAHs) have been detected in marine and terrestrial ecosystems and cause toxicological effects. These micropollutants persist in the environment for a longer duration and undergo bioaccumulation in the food chain in an ecosystem. Bioremediation is a sustainable technology for the abatement of these targeted pollutants in naturally contaminated sites. Bioremediation is a method of using microorganisms or plants for the removal of organic pollutants. It is one of the cost-effective and advantageous techniques over conventional physical and chemical methods. In this chapter, we are discussing exposure and fate of these pollutants among invertebrates, vertebrates, plants, crops, and humans. Potential risk factors, abatement of organic micropollutants through the bioremediation process, and future strategy have also been highlighted.
Shelyakin M.A., Silina E.V., Golovko T.K.
Ontogenetic development and environmental conditions are the main factors determining the life activity of plants. The activity of antioxidant enzymes and respiratory pathways in the rosette leaves of the summer-winter-green herbaceous perennial Ajuga reptans L. (common bugle) was studied in connection with age and overwintering. The leaves formed in May–June overwintered and functioned again until a new generation of leaves appeared. The content of lipid peroxidation products and hydrogen peroxide in the leaves of overwintered plants was significantly higher than the content before overwintering. The activity of antioxidant enzymes changed in different directions. An increased level of activity of ascorbate peroxidase (APX) was noted immediately after plants emerged from the snow, the catalase (CAT) activity increased before and during overwintering, and the superoxide dismutase (SOD) activity increased before, during, and immediately after overwintering. Mn-SOD, Fe-SOD, and three Cu/Zn-SOD isoforms, two APX isoforms, and one CAT isoform were identified on native gels. The respiration rate, measured at 20°C, was maximum in young growing leaves and decreased by three to four times towards the end of the life cycle. The ratio of the cytochrome and alternative respiratory pathways capacity varied from three to one or lower. The coefficient of energy efficiency of respiration (YATP/glucose, the number of moles of ATP formed during the oxidation of 1 mole of glucose in respiration) varied from 17 to 25, decreasing during the overwintering period and at the final stages of ontogenesis. The results of principal component analysis indicate the relationship between the studied parameters and the involvement of regular changes in pro-/antioxidant metabolism and respiration in the process of adaptation of plants overwintering with green leaves. Taken together, the obtained data complement and deepen the understanding of the physiological mechanisms that contribute to overwintering and preservation of the photosynthetic apparatus.
Kira R., Vitaly N., Nadezhda S.
Ma B., Shi J., Zhang Y., Li Z., Yong H., Zhou Y., Liu S., Aaa S., Zhou D.
AbstractThe irregular expression or activity of enzymes in the human body leads to various pathological disorders and can therefore be used as an intrinsic trigger for more precise identification of disease foci and controlled release of diagnostics and therapeutics, leading to improved diagnostic accuracy, sensitivity, and therapeutic efficacy while reducing systemic toxicity. Advanced synthesis strategies enable the preparation of polymers with enzymatically activatable skeletons or side chains, while understanding enzymatically responsive mechanisms promotes rational incorporation of activatable units and predictions of the release profile of diagnostics and therapeutics, ultimately leading to promising applications in disease diagnosis and treatment with superior biocompatibility and efficiency. By overcoming the challenges, new opportunities will emerge to inspire researchers to develop more efficient, safer, and clinically reliable enzymatically activatable polymeric carriers as well as prodrugs.This article is protected by copyright. All rights reserved
The oxidation of steroid derivatives by the CYP125A6 and CYP125A7 enzymes from Mycobacterium marinum
Ghith A., Bell S.G.
The members of the bacterial cytochrome P450 (CYP) monooxygenase family CYP125, catalyze the oxidation of steroid derivatives including cholesterol and phytosterols, as the initial activating step in their catabolism. However, several bacterial species contain multiple genes encoding CYP125 enzymes and other CYP enzymes which catalyze cholesterol/cholest-4-en-3-one hydroxylation. An important question is why these bacterium have more than one enzyme with overlapping substrate ranges capable of catalyzing the terminal oxidation of the alkyl chain of these sterols. To further understand the role of these enzymes we investigated CYP125A6 and CYP125A7 from Mycobacterium marinum with various cholesterol analogues. These have modifications on the A and B rings of the steroid and we assessed the substrate binding and catalytic activity of these with each enzyme. CYP125A7 gave similar results to those reported for the CYP125A1 enzyme from M. tuberculosis. Differences in the substrate binding and catalytic activity with the cholesterol analogues were observed with CYP125A6. For example, while cholesteryl sulfate could bind to both enzymes it was only oxidized by CYP125A6 and not by CYP125A7. CYP125A6 generated higher levels of metabolites with the majority of C-3 and C-7 substituted cholesterol analogues such 7-ketocholesterol. However, 5α-cholestan-3β-ol was only oxidized by CYP125A7 enzyme. The cholest-4-en-3-one and 7-ketocholesterol-bound forms of the CYP125A6 and CYP125A7 enzymes were modelled using AlphaFold. The structural models highlighted differences in the binding modes of the steroid derivatives within the same enzyme. Significant changes in the binding mode of the steroids between these CYP125 enzymes and other bacterial cholesterol oxidizing enzymes, CYP142A3 and CYP124A1, were also seen. Despite this, all these models predicted the selectivity for terminal methyl hydroxylation, in agreement with the experimental data.
Molchanov V., Yegorov A., Molchanov M., Timchenko A., Novikov V., Novojilov N., Timchenko M.
Crustaceans have successfully adapted to survive in their natural habitat, rich in microorganisms, due to the presence of antimicrobial peptides (AMPs) in their organism. They achieve this adaptation despite lacking the highly specific adaptive immune system found in vertebrates. One valuable source of AMPs is the hepatopancreas, a waste product from crab fishery and its processing. Applying zymographic and spectrophotometric techniques, we discovered a small peptide (approximately 5 kDa) within a low molecular weight protein fraction extracted from the acetone powder of the red king crab hepatopancreas. This peptide hydrolyzes both M. lysodeikticus cell wall and M. lysodeikticus cell wall polysaccharide, while showing no activity against gelatin. The found peptide may be of interest for application in medicine, biotechnology, and the food industry, for example as a bio-preservative.
Donova M.V.
The microbiological transformation of sterols is currently the technological basis for the industrial production of valuable steroid precursors, the so-called synthons, from which a wide range of steroid and indane isoprenoids are obtained by combined chemical and enzymatic routes. These compounds include value-added corticoids, neurosteroids, sex hormones, bile acids, and other terpenoid lipids required by the medicine, pharmaceutical, food, veterinary, and agricultural industries. Progress in understanding the molecular mechanisms of microbial degradation of steroids, and the development and implementation of genetic technologies, opened a new era in steroid biotechnology. Metabolic engineering of microbial producers makes it possible not only to improve the biocatalytic properties of industrial strains by enhancing their target activity and/or suppressing undesirable activities in order to avoid the formation of by-products or degradation of the steroid core, but also to redirect metabolic fluxes in cells towards accumulation of new metabolites that may be useful for practical applications. Along with whole-cell catalysis, the interest of researchers is growing in enzymatic methods that make it possible to carry out selective structural modifications of steroids, such as the introduction of double bonds, the oxidation of steroidal alcohols, or the reduction of steroid carbonyl groups. A promising area of research is strain engineering based on the heterologous expression of foreign steroidogenesis systems (bacterial, fungal, or mammalian) that ensure selective formation of demanded hydroxylated steroids. Here, current trends and progress in microbial steroid biotechnology over the past few years are briefly reviewed, with a particular focus on the application of metabolic engineering and synthetic biology techniques to improve existing and create new whole-cell microbial biocatalysts.
Sun S., Wang Y., Chen S., Li Z., Zhao C., Liu Q., Zhang X.
Engineered bacteria are one of the greenest and most profitable biodegradation methods for removing noxious chlorophenols and chloramines from the environment. This study aims to acquire an engineering isolate Pseudomonas P1 that can effectively degrade 4-Chloroaniline (4-CA) and p-Chlorophenol (4-CP) simultaneously, and reveal potential differences in core gene expression and metabolic pathways in practical applications. A protein interaction network diagram was drawn using networkX under Python to count various topological properties of genes interaction network based on whole genome sequencing to reveal the interaction between proteins involved in organochlorine degradation. The core genes expression levels during aerobic degradation of the aforementioned compounds by Pseudomonas P1, which the most excellent degradation specifications at 30 °C, neutral pH and 100 mg/L, was analyzed based on transcriptome sequencing. Importantly, two major pathways exist in Pseudomonas P1 based on transcriptome sequencing during aerobic degradation of the aforementioned compounds. 4-chlorocatechol (4-CC) pathway of 4-CP degradation by multicomponent phenol hydroxylase (DmpONMLKP) and catechol 2,3-dioxygenase (DmpB), which expression levels were notably up-regulated 1.1–3.25 folds and DmpB was the most significant increased. GSH-dependent dehalogenation pathway including GSR, acpD, ahpF, yghU and katE genes to degrade 4-CA, which expression levels were significantly up-regulated 1.0–3.5 folds. The core genes expression changes were further verified by RTq-PCR. Collectively, our results provided an engineered bacteria that can efficiently mineralize 4-CA and 4-CP simultaneously, and clarified that it has two metabolic pathways regulate biodegradation of 4-CA and 4-CP, which can be practiced for in-site bioremediation of chlorophenols and chloramines co-contaminated soil.
The oxidation of cholesterol derivatives by the CYP124 and CYP142 enzymes from Mycobacterium marinum
Ghith A., Bruning J.B., Bell S.G.
The CYP124 and CYP142 families of bacterial cytochrome P450 monooxygenases (CYPs), catalyze the oxidation of methyl branched lipids, including cholesterol, as one of the initial activating steps in their catabolism. Both enzymes are reported to supplement the CYP125 family of P450 enzymes. These CYP125 enzymes are found in the same bacteria, and are the primary cholesterol/cholest-4-en-3-one metabolizing enzymes. To further understand the role of the CYP124 and CYP142 cytochrome P450s we investigated the Mycobacterium marinum enzymes, MmarCYP124A1 and CYP142A3, with various cholesterol analogs with modifications on the A and B rings of the steroid. We assessed the substrate binding and catalytic activity of each enzyme. Neither enzyme could bind or oxidize cholesteryl acetate or 3,5-cholestadiene, which have modifications at the C3 hydroxyl moiety of cholesterol. The CYP142 enzyme was better able to accommodate and oxidize cholesterol analogs which have changes on the A/B rings including cholesterol-5α,6α-epoxide and diastereomers of 5-cholestan-3-ol. The CYP124 enzyme was more tolerant of changes at C7 of the cholesterol B ring, e.g., 7-ketocholesterol than in the A ring. The selectivity for oxidation at the ω-carbon of a branched chain was observed in all steroids that were oxidized. The 7-ketocholesterol-bound MmarCYP124A1 enzyme from M. marinum, was structurally characterized by X-ray crystallography to 1.81 Å resolution. The 7-ketocholesterol-bound X-ray crystal structure of the MmarCYP124A1 enzyme revealed that the substrate binding mode of this cholesterol derivative was altered compared to those observed with other non-steroidal ligands. The structure provided an explanation for the selectivity of the enzyme for terminal methyl hydroxylation.
Vetoshkina D., Balashov N., Ivanov B., Ashikhmin A., Borisova-Mubarakshina M.
Light harvesting is finetuned through two main strategies controlling energy transfer to the reaction centers of photosystems: i) regulating the amount of light energy at the absorption level, ii) regulating the amount of the absorbed energy at the utilization level. The first strategy is ensured by changes in the cross-section, i.e., the size of the photosynthetic antenna. These changes can occur in a short-term (state transitions) or long-term way (changes in antenna protein biosynthesis) depending on the light conditions. The interrelation of these two ways is still underexplored. Regulating light absorption through the long-term modulation of photosystem II antenna size has been mostly considered as an acclimatory mechanism to light conditions. The present review highlights that this mechanism represents one of the most versatile mechanisms of higher plant acclimation to various conditions including drought, salinity, temperature changes, and even biotic factors. We suggest that H2O2 is the universal signaling agent providing the switch from the short-term to long-term modulation of photosystem II antenna size under these factors. The second strategy of light harvesting is represented by redirecting energy to waste mainly via thermal energy dissipation in the photosystem II antenna in high light through PsbS protein and xanthophyll cycle. In the latter case, H2O2 also plays a considerable role. This circumstance may explain the maintenance of the appropriate level of zeaxanthin not only upon high light but also upon other stress factors. Thus, the review emphasizes the significance of both strategies for ensuring plant sustainability under various environmental conditions.
Pang B., Wang H., Huang H., Liao L., Wang Y., Wang M., Du G., Kang Z.
Hyaluronic acid (HA) is a nonsulfated linear glycosaminoglycan with a negative charge. Different from the high-molecular-weight HAs, the low-molecular-weight HAs (LMW-HAs, 4-120 kDa) and hyaluronan oligosaccharides (O-HAs,
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López C., Delmonti J., Bonomo R.A., Vila A.J.
Understanding the evolution of metallo-β-lactamases (MBLs) is fundamental to deciphering the mechanistic basis of resistance to carbapenems in pathogenic and opportunistic bacteria. Presently, these MBL-producing pathogens are linked to high rates of morbidity and mortality worldwide. However, the study of the biochemical and biophysical features of MBLs in vitro provides an incomplete picture of their evolutionary potential, since this limited and artificial environment disregards the physiological context where evolution and selection take place. Herein, we describe recent efforts aimed to address the evolutionary traits acquired by different clinical variants of MBLs in conditions mimicking their native environment (the bacterial periplasm) and considering whether they are soluble or membrane-bound proteins. This includes addressing the metal content of MBLs within the cell under zinc starvation conditions and the context provided by different bacterial hosts that result in particular resistance phenotypes. Our analysis highlights recent progress bridging the gap between in vitro and in-cell studies.
Sindelar M., Jilkova J., Kubala L., Velebny V., Turkova K.
• Applications of hyaluronan require knowledge of its effective enzymatic degradation. • Hyaluronan is depolymerized by enzymes isolated across all levels of life. • Annotated hyaluronidases and hyaluronate lyases possess low sequence homology. • Hyaluronidase and hyaluronate lyases belong to distinct CAZy families. Hyaluronan is a non-sulfated negatively-charged linear polymer distributed in most parts of the human body, where it is located around cells in the extracellular matrix of connective tissues and plays an essential role in the organization of tissue architecture. Moreover, hyaluronan is involved in many biological processes and used in many clinical, cosmetic, pharmaceutic, and biotechnological applications worldwide. As interest in hyaluronan applications increases, so does interest in hyaluronidases and hyaluronate lyases, as these enzymes play a major part in hyaluronan degradation. Many hyaluronidases and hyaluronate lyases produced by eukaryotic cells, bacteria, and bacteriophages have so far been described and annotated, and their ability to cleave hyaluronan has been experimentally proven. These enzymes belong to several carbohydrate-active enzyme families, share very low sequence identity, and differ in their cleaving mechanisms and in their structural and functional properties. This review presents a summary of annotated and characterized hyaluronidases and hyaluronate lyases isolated from different sources belonging to distinct protein families, with a main focus on the binding and catalytic residues of the discussed enzymes in the context of their biochemical properties. In addition, the application potential of individual groups of hyaluronidases and hyaluronate lyases is evaluated.
Lobastova T.G., Fokina V.V., Tarlachkov S.V., Shutov A.A., Bragin E.Y., Kazantsev A.V., Donova M.V.
Abstract
Application of thermophile microorganisms opens new prospects in steroid biotechnology, however little is known on steroid catabolism by the thermophile strains.The thermophilic Saccharopolyspora hirsuta subsp. hirsuta strain VKM Ac-666T is capable of structural modification of different steroids, and fully degrades cholesterol. The intermediates of the cholesterol degradation pathway were identified as cholest-4-en-3-one, cholesta-1,4-dien-3-one, 26-hydroxycholest-4-en-3-one, 3-oxo-cholest-4-en-26-oic acid, 3-oxo-cholesta-1,4-dien-26-oic acid, 26-hydroxycholesterol, 3β-hydroxy-cholest-5-en-26-oic acid by MS, and H1- and C13-NMR analyses. The data evidence sterol degradation by the strain occurs simultaneously through the aliphatic side chain hydroxylation at C26 and the A-ring modification that are putatively catalyzed by cytochrome P450 monooxygenase CYP125 and cholesterol oxidase, respectively.The genes orthologous to those related to the sterol side chain degradation, steroid core rings A/B and C/D disruption and the steroid uptake were revealed. Most of the genes related to steroid degradation are grouped in three clusters. The sets of the genes putatively involved in steroid catabolism and peculiarities of their organization in S. hirsuta are discussed.Despite steroids abundancy in the environments, the ability to degrade them is not widespread among thermophilic bacteria as follows from the bioinformatic analysis of 52 publicly available genomes. Only seven candidate strains were revealed to possess the key genes related to the only known 9(10)-seco pathway of steroid degradation.The results contribute to the knowledge on diversity of microbial steroid degraders, the features of sterol catabolism by thermophilic actinobacteria and could be useful for application in the pharmaceutical and environmental biotechnology.
Bahr G., González L.J., Vila A.J.
Antimicrobial resistance is one of the major problems in current practical medicine. The spread of genes coding for resistance determinants among bacteria challenges the use of approved antibiotics, narrowing the options for treatment. Resistance to carbapenems, last resort antibiotics, is a major concern. Metallo-β-lactamases (MBLs) hydrolyze carbapenems, penicillins, and cephalosporins, becoming central to this problem. These enzymes diverge with respect to serine-β-lactamases by exhibiting a different fold, active site, and catalytic features. Elucidating their catalytic mechanism has been a big challenge in the field that has limited the development of useful inhibitors. This review covers exhaustively the details of the active-site chemistries, the diversity of MBL alleles, the catalytic mechanism against different substrates, and how this information has helped developing inhibitors. We also discuss here different aspects critical to understand the success of MBLs in conferring resistance: the molecular determinants of their dissemination, their cell physiology, from the biogenesis to the processing involved in the transit to the periplasm, and the uptake of the Zn(II) ions upon metal starvation conditions, such as those encountered during an infection. In this regard, the chemical, biochemical and microbiological aspects provide an integrative view of the current knowledge of MBLs.
Pawełczyk J., Brzostek A., Minias A., Płociński P., Rumijowska-Galewicz A., Strapagiel D., Zakrzewska-Czerwińska J., Dziadek J.
Mycobacterium tuberculosis (Mtb) is an obligate human pathogen that can adapt to the various nutrients available during its life cycle. However, in the nutritionally stringent environment of the macrophage phagolysosome, Mtb relies mainly on cholesterol. In previous studies, we demonstrated that Mtb can accumulate and utilize cholesterol as the sole carbon source. However, a growing body of evidence suggests that a lipid-rich environment may have a much broader impact on the pathogenesis of Mtb infection than previously thought. Therefore, we applied high-resolution transcriptome profiling and the construction of various mutants to explore in detail the global effect of cholesterol on the tubercle bacillus metabolism. The results allow re-establishing the complete list of genes potentially involved in cholesterol breakdown. Moreover, we identified the modulatory effect of vitamin B12 on Mtb transcriptome and the novel function of cobalamin in cholesterol metabolite dissipation which explains the probable role of B12 in Mtb virulence. Finally, we demonstrate that a key role of cholesterol in mycobacterial metabolism is not only providing carbon and energy but involves also a transcriptome remodeling program that helps in developing tolerance to the unfavorable host cell environment far before specific stress-inducing phagosomal signals occur.
Letunic I., Bork P.
Abstract The Interactive Tree Of Life (https://itol.embl.de) is an online tool for the display, manipulation and annotation of phylogenetic and other trees. It is freely available and open to everyone. iTOL version 5 introduces a completely new tree display engine, together with numerous new features. For example, a new dataset type has been added (MEME motifs), while annotation options have been expanded for several existing ones. Node metadata display options have been extended and now also support non-numerical categorical values, as well as multiple values per node. Direct manual annotation is now available, providing a set of basic drawing and labeling tools, allowing users to draw shapes, labels and other features by hand directly onto the trees. Support for tree and dataset scales has been extended, providing fine control over line and label styles. Unrooted tree displays can now use the equal-daylight algorithm, proving a much greater display clarity. The user account system has been streamlined and expanded with new navigation options and currently handles >1 million trees from >70 000 individual users.
Shtratnikova V.Y., Sсhelkunov M.I., Fokina V.V., Bragin E.Y., Shutov A.A., Donova M.V.
Abstract
Background
Bacterial degradation/transformation of steroids is widely investigated to create biotechnologically relevant strains for industrial application. The strain of Nocardioides simplex VKM Ac-2033D is well known mainly for its superior 3-ketosteroid Δ1-dehydrogenase activity towards various 3-oxosteroids and other important reactions of sterol degradation. However, its biocatalytic capacities and the molecular fundamentals of its activity towards natural sterols and synthetic steroids were not fully understood. In this study, a comparative investigation of the genome-wide transcriptome profiling of the N. simplex VKM Ac-2033D grown on phytosterol, or in the presence of cortisone 21-acetate was performed with RNA-seq.
Results
Although the gene patterns induced by phytosterol generally resemble the gene sets involved in phytosterol degradation pathways in mycolic acid rich actinobacteria such as Mycolicibacterium, Mycobacterium and Rhodococcus species, the differences in gene organization and previously unreported genes with high expression level were revealed. Transcription of the genes related to KstR- and KstR2-regulons was mainly enhanced in response to phytosterol, and the role in steroid catabolism is predicted for some dozens of the genes in N. simplex. New transcription factors binding motifs and new candidate transcription regulators of steroid catabolism were predicted in N. simplex.
Unlike phytosterol, cortisone 21-acetate does not provide induction of the genes with predicted KstR and KstR2 sites. Superior 3-ketosteroid-Δ1-dehydrogenase activity of N. simplex VKM Ac-2033D is due to the kstDs redundancy in the genome, with the highest expression level of the gene KR76_27125 orthologous to kstD2, in response to cortisone 21-acetate. The substrate spectrum of N. simplex 3-ketosteroid-Δ1-dehydrogenase was expanded in this study with progesterone and its 17α-hydroxylated and 11α,17α-dihydroxylated derivatives, that effectively were 1(2)-dehydrogenated in vivo by the whole cells of the N. simplex VKM Ac-2033D.
Conclusion
The results contribute to the knowledge of biocatalytic features and diversity of steroid modification capabilities of actinobacteria, defining targets for further bioengineering manipulations with the purpose of expansion of their biotechnological applications.
Ponomareva T., Timchenko M., Filippov M., Lapaev S., Sogorin E.
Since the early 1980s, a large number of studies on enzymes from the red king crab hepatopancreas were conducted. They have been relevant both from a fundamental point of view in terms of studying the enzymes of marine organisms and in terms of rational natural resource management aimed to obtain new valuable products from the processing of crab fishing waste. Most of these works were performed by Russian scientists due to the area and amount of waste of red king crab processing in Russia (or the Soviet Union). However, the close phylogenetic kinship and the similar ecological niches of commercial crab species and the production scale of the catch provide the bases for the successful transfer of experience in the processing of the red king crab hepatopancreas to other commercial crab species caught worldwide. This review describes the value of recycled commercial crab species, discusses processing problems, and suggests possible solutions for these issues. The main emphasis is made on hepatopancreatic enzymes as the most salubrious products of red king crab waste processing.
Restaino O.F., Barbuto Ferraiuolo S., Perna A., Cammarota M., Borzacchiello M.G., Fiorentino A., Schiraldi C.
16α-Hydroxyprednisolone, an anti-inflammatory drug, could be potentially obtained from hydrocortisone bioconversion by combining a 1,2-dehydrogenation reaction performed by Arthrobacter simplexATCC31652 with a 16α-hydroxylation reaction by Streptomyces roseochromogenes ATCC13400. In this study we tested, for the first time, potential approaches to couple the two reactions using similar pH and temperature conditions for hydrocortisone bioconversion by the two strains. The A. simplex capability to 1,2-dehydrogenate the 16α-hydroxyhydrocortisone, the product of S. roseochromogenes transformation of hydrocortisone, and vice versa the capability of S. roseochromogenes to 16α-hydroxylate the prednisolone were assessed. Bioconversions were studied in shake flasks and strain morphology changes were observed by SEM. Whole cell experiments were set up to perform the two reactions in a sequential mode in alternate order or contemporarily at diverse temperature conditions. A. simplex catalyzed either the dehydrogenation of hydrocortisone into prednisolone efficiently or of 16α-hydroxyhydrocortisone into 16α-hydroxyprednisolone in 24 h (up to 93.9%). Surprisingly S. roseochromogenes partially converted prednisolone back to hydrocortisone. A 68.8% maximum of 16α-hydroxyprednisolone was obtained in 120-h bioconversion by coupling whole cells of the two strains at pH 6.0 and 26 °C. High bioconversion of hydrocortisone into 16α-hydroxyprednisolone was obtained for the first time by coupling A. simplex and S. roseochromogenes.
Shtratnikova V.Y., Schelkunov M.I., Fokina V.V., Bragin E.Y., Lobastova T.G., Shutov A.A., Kazantsev A.V., Donova M.V.
Steroid microbial degradation plays a significant ecological role for biomass decomposition and removal/detoxification of steroid pollutants. In this study, the initial steps of cholesterol degradation and lithocholate bioconversion by a strain with enhanced 3-ketosteroid dehydrogenase (3-KSD) activity, Nocardioides simplex VKM Ac-2033D, were studied. Biochemical, transcriptomic, and bioinformatic approaches were used. Among the intermediates of sterol sidechain oxidation cholest-5-en-26-oic acid and 3-oxo-cholesta-1,4-dien-26-oic acid were identified as those that have not been earlier reported for N. simplex and related species. The transcriptomic approach revealed candidate genes of cholesterol and lithocholic acid (LCA) catabolism by the strain. A separate set of genes combined in cluster and additional 3-ketosteroid Δ1-dehydrogenase and 3-ketosteroid 9α-hydroxylases that might be involved in LCA catabolism were predicted. Bioinformatic calculations based on transcriptomic data showed the existence of a previously unknown transcription factor, which regulates cholate catabolism gene orthologs. The results contribute to the knowledge on diversity of steroid catabolism regulation in actinobacteria and might be used at the engineering of microbial catalysts for ecological and industrial biotechnology.
Filonov A., Delegan Y., Puntus I., Valentovich L., Akhremchuk A., Evdokimova O., Funtikova T., Zakharova M., Akhmetov L., Vetrova A., Titok M.
The strain
Pseudomonas putida
BS3701 was isolated from soil contaminated with coke by-product waste (Moscow Region, Russian Federation). It is capable of degrading crude oil and polycyclic aromatic hydrocarbons (PAHs). The
P. putida
BS3701 genome consists of a 6,337,358-bp circular chromosome and two circular plasmids (pBS1141 with 107,388 bp and pBS1142 with 54,501 bp).
Gadbery J.E., Round J.W., Yuan T., Wipperman M.F., Story K.T., Crowe A.M., Casabon I., Liu J., Yang X., Eltis L.D., Sampson N.S.
Steroid-degrading bacteria, including Mycobacterium tuberculosis (Mtb), utilize an architecturally distinct subfamily of acyl coenzyme A dehydrogenases (ACADs) for steroid catabolism. These ACADs are α2β2 heterotetramers that are usually encoded by adjacent fadE-like genes. In mycobacteria, ipdE1 and ipdE2 (formerly fadE30 and fadE33) occur in divergently transcribed operons associated with the catabolism of 3aα-H-4α(3′-propanoate)-7aβ-methylhexahydro-1,5-indanedione (HIP), a steroid metabolite. In Mycobacterium smegmatis, ΔipdE1 and ΔipdE2 mutants had similar phenotypes, showing impaired growth on cholesterol and accumulating 5-OH HIP in the culture supernatant. Bioinformatic analyses revealed that IpdE1 and IpdE2 share many of the features of the α- and β-subunits, respectively, of heterotetrameric ACADs that are encoded by adjacent genes in many steroid-degrading proteobacteria. When coproduced in a rhodococcal strain, IpdE1 and IpdE2 of Mtb formed a complex that catalyzed the dehydrogenation of 5OH-HIP coenzyme A (5OH-HIP-CoA) to 5OH-3aα-H-4α(3′-prop-1-enoate)-7aβ-methylhexa-hydro-1,5-indanedione coenzyme A ((E)-5OH-HIPE-CoA). This corresponds to the initial step in the pathway that leads to degradation of steroid C and D rings via β-oxidation. Small-angle X-ray scattering revealed that the IpdE1-IpdE2 complex was an α2β2 heterotetramer typical of other ACADs involved in steroid catabolism. These results provide insight into an important class of steroid catabolic enzymes and a potential virulence determinant in Mtb.
Ponomareva T., Sliadovskii D., Timchenko M., Molchanov M., Timchenko A., Sogorin E.
In this study, several methods were used to analyze the hydrolysis of hyaluronic acid (HA)-based cosmetic fillers by the hepatopancreas homogenate of the Red king crab. The results show that the homogenate and commercially available hyaluronidases have similar hydrolysis activities on the fillers. Atomic force microscopy images reveal that the HA fillers consist mainly of spherical-like particles, which are converted into filamentous structures as a result of hydrolysis by the Red king crab hepatopancreas homogenate. Turbidimetric analysis of the hydrolysis process shows that HA aggregation with acidic albumin exhibits a bell-shaped dependence on reaction time. Analysis of the hydrolysis process by nuclear magnetic resonance shows that HA degradation lasts several days. The maximum rate of the reaction is detected in the 1st h of incubation. The data confirm that the purified homogenate of the Red king crab hepatopancreas exerts hyaluronidase activity on HA-based cosmetic fillers; therefore, it may be considered as a potential therapeutic agent for treating filler complications.
Lobastova T.G., Fokina V.V., Bragin E.Y., Shtratnikova V.Y., Starodumova I.P., Tarlachkov S.V., Donova M.V.
The draft genome sequence of the type strain Saccharopolyspora hirsuta subsp. hirsuta VKM Ac-666 was sequenced. This moderately thermophilic actinobacterial strain of sugarcane bagasse origin is able to transform different steroid substrates. ABSTRACT The draft genome sequence of the type strain Saccharopolyspora hirsuta subsp. hirsuta VKM Ac-666 was sequenced. This moderately thermophilic actinobacterial strain of sugarcane bagasse origin is able to transform different steroid substrates.
Microbial degradation of steroid sex hormones: implications for environmental and ecological studies
Chiang Y., Wei S.T., Wang P., Wu P., Yu C.
Steroid hormones modulate development, reproduction and communication in eukaryotes. The widespread occurrence and persistence of steroid hormones have attracted public attention due to their endocrine-disrupting effects on both wildlife and human beings. Bacteria are responsible for mineralizing steroids from the biosphere. Aerobic degradation of steroid hormones relies on O2 as a co-substrate of oxygenases to activate and to cleave the recalcitrant steroidal core ring. To date, two oxygen-dependent degradation pathways - the 9,10-seco pathway for androgens and the 4,5-seco pathways for oestrogens - have been characterized. Under anaerobic conditions, denitrifying bacteria adopt the 2,3-seco pathway to degrade different steroid structures. Recent meta-omics revealed that microorganisms able to degrade steroids are highly diverse and ubiquitous in different ecosystems. This review also summarizes culture-independent approaches using the characteristic metabolites and catabolic genes to monitor steroid biodegradation in various ecosystems.
Total publications
9
Total citations
29
Citations per publication
3.22
Average publications per year
1.13
Average coauthors
4.56
Publications years
2017-2024 (8 years)
h-index
4
i10-index
0
m-index
0.5
o-index
5
g-index
5
w-index
0
Metrics description
h-index
A scientist has an h-index if h of his N publications are cited at least h times each, while the remaining (N - h) publications are cited no more than h times each.
i10-index
The number of the author's publications that received at least 10 links each.
m-index
The researcher's m-index is numerically equal to the ratio of his h-index to the number of years that have passed since the first publication.
o-index
The geometric mean of the h-index and the number of citations of the most cited article of the scientist.
g-index
For a given set of articles, sorted in descending order of the number of citations that these articles received, the g-index is the largest number such that the g most cited articles received (in total) at least g2 citations.
w-index
If w articles of a researcher have at least 10w citations each and other publications are less than 10(w+1) citations, then the researcher's w-index is equal to w.
Top-100
Fields of science
1
2
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Molecular Biology
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Molecular Biology, 2, 22.22%
Molecular Biology
2 publications, 22.22%
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General Medicine
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General Medicine, 2, 22.22%
General Medicine
2 publications, 22.22%
|
Microbiology (medical)
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Microbiology (medical), 2, 22.22%
Microbiology (medical)
2 publications, 22.22%
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Microbiology
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Microbiology, 2, 22.22%
Microbiology
2 publications, 22.22%
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Applied Microbiology and Biotechnology
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Applied Microbiology and Biotechnology, 2, 22.22%
Applied Microbiology and Biotechnology
2 publications, 22.22%
|
Catalysis
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Catalysis, 1, 11.11%
Catalysis
1 publication, 11.11%
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Organic Chemistry
|
Organic Chemistry, 1, 11.11%
Organic Chemistry
1 publication, 11.11%
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Biochemistry
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Biochemistry, 1, 11.11%
Biochemistry
1 publication, 11.11%
|
Inorganic Chemistry
|
Inorganic Chemistry, 1, 11.11%
Inorganic Chemistry
1 publication, 11.11%
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Physical and Theoretical Chemistry
|
Physical and Theoretical Chemistry, 1, 11.11%
Physical and Theoretical Chemistry
1 publication, 11.11%
|
Computer Science Applications
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Computer Science Applications, 1, 11.11%
Computer Science Applications
1 publication, 11.11%
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Spectroscopy
|
Spectroscopy, 1, 11.11%
Spectroscopy
1 publication, 11.11%
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Genetics
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Genetics, 1, 11.11%
Genetics
1 publication, 11.11%
|
Multidisciplinary
|
Multidisciplinary, 1, 11.11%
Multidisciplinary
1 publication, 11.11%
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Infectious Diseases
|
Infectious Diseases, 1, 11.11%
Infectious Diseases
1 publication, 11.11%
|
Pharmacology (medical)
|
Pharmacology (medical), 1, 11.11%
Pharmacology (medical)
1 publication, 11.11%
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Biotechnology
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Biotechnology, 1, 11.11%
Biotechnology
1 publication, 11.11%
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Bioengineering
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Bioengineering, 1, 11.11%
Bioengineering
1 publication, 11.11%
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Epidemiology
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Epidemiology, 1, 11.11%
Epidemiology
1 publication, 11.11%
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1
2
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Journals
1
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Applied Biochemistry and Microbiology
1 publication, 11.11%
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Journal of Biotechnology
1 publication, 11.11%
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Scientific Reports
1 publication, 11.11%
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International Journal of Molecular Sciences
1 publication, 11.11%
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FEMS Microbiology Letters
1 publication, 11.11%
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Frontiers in Microbiology
1 publication, 11.11%
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Clinical Microbiology and Antimicrobial Chemotherapy
1 publication, 11.11%
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1
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Citing journals
1
2
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Journal not defined
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Journal not defined, 2, 6.9%
Journal not defined
2 citations, 6.9%
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Journal of Steroid Biochemistry and Molecular Biology
2 citations, 6.9%
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Russian Journal of Plant Physiology
2 citations, 6.9%
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Applied and Environmental Microbiology
2 citations, 6.9%
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Functional Plant Biology
2 citations, 6.9%
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PeerJ
1 citation, 3.45%
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Methods in Molecular Biology
1 citation, 3.45%
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Microbiological Research
1 citation, 3.45%
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Applied Biochemistry and Microbiology
1 citation, 3.45%
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Biomolecules
1 citation, 3.45%
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International Journal of Molecular Sciences
1 citation, 3.45%
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Frontiers in Physiology
1 citation, 3.45%
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Chemical Engineering Journal
1 citation, 3.45%
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Plant Physiology and Biochemistry
1 citation, 3.45%
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Applied Microbiology and Biotechnology
1 citation, 3.45%
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AIP Conference Proceedings
1 citation, 3.45%
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Journal of Agricultural and Food Chemistry
1 citation, 3.45%
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Carbohydrate Research
1 citation, 3.45%
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Journal of Pharmaceutical and Biomedical Analysis
1 citation, 3.45%
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Journal of Hazardous Materials
1 citation, 3.45%
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Biomacromolecules
1 citation, 3.45%
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Advanced Materials
1 citation, 3.45%
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Физиология растений
1 citation, 3.45%
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Microorganisms for Sustainability
1 citation, 3.45%
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1
2
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Publishers
1
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Springer Nature
1 publication, 11.11%
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Elsevier
1 publication, 11.11%
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Oxford University Press
1 publication, 11.11%
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Pleiades Publishing
1 publication, 11.11%
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MDPI
1 publication, 11.11%
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Frontiers Media S.A.
1 publication, 11.11%
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Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy
1 publication, 11.11%
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1
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Organizations from articles
1
2
3
4
5
6
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Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences
6 publications, 28.57%
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Organization not defined
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Organization not defined, 3, 14.29%
Organization not defined
3 publications, 14.29%
|
Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences
3 publications, 14.29%
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Tula State University
2 publications, 9.52%
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Lomonosov Moscow State University
1 publication, 4.76%
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Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences
1 publication, 4.76%
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Institute of Protein Research of the Russian Academy of Sciences
1 publication, 4.76%
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Institute for Biological Instrumentation of the Russian Academy of Sciences
1 publication, 4.76%
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Institute of Basic Biological Problems of the Russian Academy of Sciences
1 publication, 4.76%
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Kazan Federal University
1 publication, 4.76%
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Juntendo University
1 publication, 4.76%
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1
2
3
4
5
6
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Countries from articles
1
2
3
4
5
6
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Russia
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Russia, 6, 60%
Russia
6 publications, 60%
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Country not defined
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Country not defined, 3, 30%
Country not defined
3 publications, 30%
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Japan
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Japan, 1, 10%
Japan
1 publication, 10%
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1
2
3
4
5
6
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Citing organizations
1
2
3
4
5
6
7
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Organization not defined
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Organization not defined, 7, 14%
Organization not defined
7 citations, 14%
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Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences
3 citations, 6%
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Nanjing Agricultural University
3 citations, 6%
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Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences
2 citations, 4%
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Institute of Protein Research of the Russian Academy of Sciences
2 citations, 4%
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Institute of Basic Biological Problems of the Russian Academy of Sciences
2 citations, 4%
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Institute of Biology Komi SC of the Ural Branch of the Russian Academy of Sciences
2 citations, 4%
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University of Adelaide
2 citations, 4%
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Lomonosov Moscow State University
1 citation, 2%
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Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
1 citation, 2%
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National Research Nuclear University MEPhI
1 citation, 2%
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Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences
1 citation, 2%
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Institute for Biological Instrumentation of the Russian Academy of Sciences
1 citation, 2%
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Institute for High Energy Physics of NRC «Kurchatov Institute»
1 citation, 2%
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All-Russian Institute of Plant Protection
1 citation, 2%
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All-Russian Research Institute Fisheries and Oceanography
1 citation, 2%
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All-Russian Research Institute of Agricultural Microbiology
1 citation, 2%
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Isfahan University of Technology
1 citation, 2%
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Indian Institute of Technology Kanpur
1 citation, 2%
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Graphic Era Hill University
1 citation, 2%
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Shahrekord University
1 citation, 2%
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Assam University
1 citation, 2%
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Tsinghua University
1 citation, 2%
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Zhejiang University
1 citation, 2%
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Xi'an Jiaotong University
1 citation, 2%
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Govind Ballabh Pant University of Agriculture and Technology
1 citation, 2%
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China University of Petroleum (East China)
1 citation, 2%
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Shandong University of Science and Technology
1 citation, 2%
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China Agricultural University
1 citation, 2%
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Jiangnan University
1 citation, 2%
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Nantong University
1 citation, 2%
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Anhui University of Science and Technology
1 citation, 2%
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Chung-Ang University
1 citation, 2%
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Yeungnam University
1 citation, 2%
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Shizuoka University
1 citation, 2%
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Show all (5 more) | |
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Citing countries
1
2
3
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8
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Russia
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Russia, 8, 26.67%
Russia
8 citations, 26.67%
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China
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China, 7, 23.33%
China
7 citations, 23.33%
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Country not defined
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Country not defined, 4, 13.33%
Country not defined
4 citations, 13.33%
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India
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India, 3, 10%
India
3 citations, 10%
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Australia
|
Australia, 2, 6.67%
Australia
2 citations, 6.67%
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Republic of Korea
|
Republic of Korea, 2, 6.67%
Republic of Korea
2 citations, 6.67%
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Iran
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Iran, 1, 3.33%
Iran
1 citation, 3.33%
|
Ireland
|
Ireland, 1, 3.33%
Ireland
1 citation, 3.33%
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Poland
|
Poland, 1, 3.33%
Poland
1 citation, 3.33%
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Japan
|
Japan, 1, 3.33%
Japan
1 citation, 3.33%
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1
2
3
4
5
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7
8
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- We do not take into account publications without a DOI.
- Statistics recalculated daily.
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Company/Organization
Position
Junior Researcher
Employment type
Full time
Years
2014 —
present
Company/Organization
Position
Head of Educational Laboratories
Employment type
Full time
Years
2012 —
2014