Cellular and Molecular Life Sciences, volume 77, issue 21, pages 4429-4440
Highly photostable fluorescent labeling of proteins in live cells using exchangeable coiled coils heterodimerization
Perfilov Maxim M
1
,
GURSKAYA Nadya G.
1, 2
,
Serebrovskaya Ekaterina O.
1, 3
,
Melnikov Pavel A
4
,
Kharitonov Sergey L.
1
,
Lewis Tylor R
3
,
ARSHAVSKY Vadim Y.
3, 5
,
Baklaushev Vladimir P.
6
,
Lukyanov Konstantin A.
1, 7
3
Department of Ophthalmology, Duke University, Durham, USA
|
5
Department of Pharmacology, Duke University, Durham, USA
|
6
Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies, FMBA of Russia, Moscow, Russia
|
Publication type: Journal Article
Publication date: 2020-01-02
Journal:
Cellular and Molecular Life Sciences
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 8
ISSN: 1420682X, 14209071
PubMed ID:
31894363
Molecular Biology
Pharmacology
Cell Biology
Molecular Medicine
Cellular and Molecular Neuroscience
Abstract
Fluorescent proteins are commonly used to label target proteins in live cells. However, the conventional approach based on covalent fusion of targeted proteins with fluorescent protein probes is limited by the slow rate of fluorophore maturation and irretrievable loss of fluorescence due to photobleaching. Here, we report a genetically encoded protein labeling system utilizing transient interactions of small, 21–28 residues-long helical protein tags (K/E coils, KEC). In this system, a protein of interest, covalently tagged with a single coil, is visualized through binding to a cytoplasmic fluorescent protein carrying a complementary coil. The reversible heterodimerization of KECs, whose affinity can be tuned in a broad concentration range from nanomolar to micromolar, allows continuous exchange and replenishment of the tag bound to a targeted protein with the entire cytosolic pool of soluble fluorescent coils. We found that, under conditions of partial illumination of living cells, the photostability of labeling with KECs exceeds that of covalently fused fluorescent probes by approximately one order of magnitude. Similarly, single-molecule localization microscopy with KECs provided higher labeling density and allowed a much longer duration of imaging than with conventional fusion to fluorescent proteins. We also demonstrated that this method is well suited for imaging newly synthesized proteins, because the labeling efficiency by KECs is not dependent on the rate of fluorescent protein maturation. In conclusion, KECs can be used to visualize various target proteins which are directly exposed to the cytosol, thereby enabling their advanced characterization in time and space.
Citations by journals
|
1
2
|
|
|
International Journal of Molecular Sciences
|
International Journal of Molecular Sciences
2 publications, 22.22%
|
|
Communications Biology
|
Communications Biology
1 publication, 11.11%
|
|
Nano Letters
|
Nano Letters
1 publication, 11.11%
|
|
Small
|
Small
1 publication, 11.11%
|
|
Nature Communications
|
Nature Communications
1 publication, 11.11%
|
|
Current Opinion in Chemical Biology
|
Current Opinion in Chemical Biology
1 publication, 11.11%
|
|
1
2
|
Citations by publishers
|
1
2
|
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 22.22%
|
|
Springer Nature
|
Springer Nature
2 publications, 22.22%
|
|
American Chemical Society (ACS)
|
American Chemical Society (ACS)
1 publication, 11.11%
|
|
Wiley
|
Wiley
1 publication, 11.11%
|
|
Elsevier
|
Elsevier
1 publication, 11.11%
|
|
1
2
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2020,2021,2022,2023,2024],"ids":[0,0,0,0,0],"codes":[0,0,0,0,0],"imageUrls":["","","","",""],"datasets":[{"label":"Citations number","data":[2,3,0,3,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["22.22","33.33",0,"33.33","11.11"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["International Journal of Molecular Sciences","Communications Biology","Nano Letters","Small","Nature Communications","Current Opinion in Chemical Biology"],"ids":[14627,11778,10312,9872,3231,5052],"codes":[0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp"],"datasets":[{"label":"","data":[2,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[22.22,11.11,11.11,11.11,11.11,11.11],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Multidisciplinary Digital Publishing Institute (MDPI)","Springer Nature","American Chemical Society (ACS)","Wiley","Elsevier"],"ids":[202,8,40,11,17],"codes":[0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp"],"datasets":[{"label":"","data":[2,2,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[22.22,22.22,11.11,11.11,11.11],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Perfilov M. M. et al. Highly photostable fluorescent labeling of proteins in live cells using exchangeable coiled coils heterodimerization // Cellular and Molecular Life Sciences. 2020. Vol. 77. No. 21. pp. 4429-4440.
GOST all authors (up to 50)
Copy
Perfilov M. M., GURSKAYA N. G., Serebrovskaya E. O., Melnikov P. A., Kharitonov S. L., Lewis T. R., ARSHAVSKY V. Y., Baklaushev V. P., Mishin A. S., Lukyanov K. A. Highly photostable fluorescent labeling of proteins in live cells using exchangeable coiled coils heterodimerization // Cellular and Molecular Life Sciences. 2020. Vol. 77. No. 21. pp. 4429-4440.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1007/s00018-019-03426-5
UR - https://doi.org/10.1007%2Fs00018-019-03426-5
TI - Highly photostable fluorescent labeling of proteins in live cells using exchangeable coiled coils heterodimerization
T2 - Cellular and Molecular Life Sciences
AU - Melnikov, Pavel A
AU - Kharitonov, Sergey L.
AU - Lewis, Tylor R
AU - ARSHAVSKY, Vadim Y.
AU - Baklaushev, Vladimir P.
AU - Mishin, Alexander S.
AU - Lukyanov, Konstantin A.
AU - Perfilov, Maxim M
AU - GURSKAYA, Nadya G.
AU - Serebrovskaya, Ekaterina O.
PY - 2020
DA - 2020/01/02 00:00:00
PB - Springer Nature
SP - 4429-4440
IS - 21
VL - 77
PMID - 31894363
SN - 1420-682X
SN - 1420-9071
ER -
Cite this
BibTex
Copy
@article{2020_Perfilov,
author = {Pavel A Melnikov and Sergey L. Kharitonov and Tylor R Lewis and Vadim Y. ARSHAVSKY and Vladimir P. Baklaushev and Alexander S. Mishin and Konstantin A. Lukyanov and Maxim M Perfilov and Nadya G. GURSKAYA and Ekaterina O. Serebrovskaya},
title = {Highly photostable fluorescent labeling of proteins in live cells using exchangeable coiled coils heterodimerization},
journal = {Cellular and Molecular Life Sciences},
year = {2020},
volume = {77},
publisher = {Springer Nature},
month = {jan},
url = {https://doi.org/10.1007%2Fs00018-019-03426-5},
number = {21},
pages = {4429--4440},
doi = {10.1007/s00018-019-03426-5}
}
Cite this
MLA
Copy
Perfilov, Maxim M., et al. “Highly photostable fluorescent labeling of proteins in live cells using exchangeable coiled coils heterodimerization.” Cellular and Molecular Life Sciences, vol. 77, no. 21, Jan. 2020, pp. 4429-4440. https://doi.org/10.1007%2Fs00018-019-03426-5.