Amino acid analysis utilizing phenylisothiocyanate derivatives.
Steven R. Cohen
1
,
Daniel J Strydom
2, 3
1
Waters Chromatography Division of Millipore Corporation, Milford, Massachusetts 01757.
|
Publication type: Journal Article
Publication date: 1988-10-01
scimago Q3
wos Q3
SJR: 0.499
CiteScore: 5.3
Impact factor: 2.5
ISSN: 00032697, 10960309
PubMed ID:
3064649
Biochemistry
Molecular Biology
Cell Biology
Biophysics
Abstract
Advances in liquid chromatography have brought about the development of new techniques in amino acid analysis which take full advantage of precolumn derivatization procedures. Using phenylisothiocyanate as the reagent, detection limits under 1 pmol can be routinely achieved, allowing the analysis of submicrogram protein samples. Analysis times as short as 10 min for samples after hydrolysis and 1 h for physiologic samples are possible. Accurate, reproducible quantitation of amino acids can be obtained from complex matrices such as plasma, urine, feed, and food samples. This level of performance and flexibility gives the analyst the first realistic alternative to ion-exchange analysis without compromising desirable features of the traditional methodology.
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341
Total citations:
341
Citations from 2024:
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(7%)
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GOST
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Cohen S. A. et al. Amino acid analysis utilizing phenylisothiocyanate derivatives. // Analytical Biochemistry. 1988. Vol. 174. No. 1. pp. 1-16.
GOST all authors (up to 50)
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Cohen S. R., Strydom D. J. Amino acid analysis utilizing phenylisothiocyanate derivatives. // Analytical Biochemistry. 1988. Vol. 174. No. 1. pp. 1-16.
Cite this
RIS
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TY - JOUR
DO - 10.1016/0003-2697(88)90512-X
UR - https://doi.org/10.1016/0003-2697(88)90512-X
TI - Amino acid analysis utilizing phenylisothiocyanate derivatives.
T2 - Analytical Biochemistry
AU - Cohen, Steven R.
AU - Strydom, Daniel J
PY - 1988
DA - 1988/10/01
PB - Elsevier
SP - 1-16
IS - 1
VL - 174
PMID - 3064649
SN - 0003-2697
SN - 1096-0309
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{1988_Cohen,
author = {Steven R. Cohen and Daniel J Strydom},
title = {Amino acid analysis utilizing phenylisothiocyanate derivatives.},
journal = {Analytical Biochemistry},
year = {1988},
volume = {174},
publisher = {Elsevier},
month = {oct},
url = {https://doi.org/10.1016/0003-2697(88)90512-X},
number = {1},
pages = {1--16},
doi = {10.1016/0003-2697(88)90512-X}
}
Cite this
MLA
Copy
Cohen, Steven A., et al. “Amino acid analysis utilizing phenylisothiocyanate derivatives..” Analytical Biochemistry, vol. 174, no. 1, Oct. 1988, pp. 1-16. https://doi.org/10.1016/0003-2697(88)90512-X.