Alkaline phosphatase labeled SERS active sandwich immunoassay for detection of Escherichia coli
Akif Göktuğ Bozkurt
1
,
Guluzar Gorkem Buyukgoz
1
,
Mehmet Soforoglu
1
,
Ugur Tamer
2
,
Zekiye Suludere
3
,
İsmail Hakkı Boyacı
1
Publication type: Journal Article
Publication date: 2018-04-01
scimago Q2
wos Q1
SJR: 0.664
CiteScore: 8.5
Impact factor: 4.6
ISSN: 13861425, 18733557
PubMed ID:
29306060
Spectroscopy
Analytical Chemistry
Atomic and Molecular Physics, and Optics
Instrumentation
Abstract
In this study, a sandwich immunoassay method utilizing enzymatic activity of alkaline phosphatase (ALP) on 5-bromo-4-chloro-3-indolyl phosphate (BCIP) for Escherichia coli (E. coli) detection was developed using surface enhanced Raman spectroscopy (SERS). For this purpose, spherical magnetic gold coated core-shell nanoparticles (MNPs-Au) and rod shape gold nanoparticles (Au-NRs) were synthesized and modified for immunomagnetic separation (IMS) of E. coli from the solution. In order to specify the developed method to ALP activity, Au-NRs were labeled with this enzyme. After successful construction of the immunoassay, BCIP substrate was added to produce the SERS-active product; 5-bromo-4-chloro-3-indole (BCI). A good linearity (R2=0.992) was established between the specific SERS intensity of BCI at 600cm-1 and logarithmic E. coli concentration in the range of 1.7×101-1.7×106cfumL-1. LOD and LOQ values were also calculated and found to be 10cfumL-1 and 30cfumL-1, respectively.
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Bozkurt A. G. et al. Alkaline phosphatase labeled SERS active sandwich immunoassay for detection of Escherichia coli // Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. 2018. Vol. 194. pp. 8-13.
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Bozkurt A. G., Buyukgoz G. G., Soforoglu M., Tamer U., Suludere Z., Boyacı İ. H. Alkaline phosphatase labeled SERS active sandwich immunoassay for detection of Escherichia coli // Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. 2018. Vol. 194. pp. 8-13.
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TY - JOUR
DO - 10.1016/j.saa.2017.12.057
UR - https://doi.org/10.1016/j.saa.2017.12.057
TI - Alkaline phosphatase labeled SERS active sandwich immunoassay for detection of Escherichia coli
T2 - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
AU - Bozkurt, Akif Göktuğ
AU - Buyukgoz, Guluzar Gorkem
AU - Soforoglu, Mehmet
AU - Tamer, Ugur
AU - Suludere, Zekiye
AU - Boyacı, İsmail Hakkı
PY - 2018
DA - 2018/04/01
PB - Elsevier
SP - 8-13
VL - 194
PMID - 29306060
SN - 1386-1425
SN - 1873-3557
ER -
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@article{2018_Bozkurt,
author = {Akif Göktuğ Bozkurt and Guluzar Gorkem Buyukgoz and Mehmet Soforoglu and Ugur Tamer and Zekiye Suludere and İsmail Hakkı Boyacı},
title = {Alkaline phosphatase labeled SERS active sandwich immunoassay for detection of Escherichia coli},
journal = {Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy},
year = {2018},
volume = {194},
publisher = {Elsevier},
month = {apr},
url = {https://doi.org/10.1016/j.saa.2017.12.057},
pages = {8--13},
doi = {10.1016/j.saa.2017.12.057}
}