том 136 издание 28 страницы 9874-9877

Physiological-Temperature Distance Measurement in Nucleic Acid using Triarylmethyl-Based Spin Labels and Pulsed Dipolar EPR Spectroscopy

Тип публикацииJournal Article
Дата публикации2014-07-01
scimago Q1
wos Q1
БС1
SJR5.554
CiteScore22.5
Impact factor15.6
ISSN00027863, 15205126
General Chemistry
Catalysis
Biochemistry
Colloid and Surface Chemistry
Краткое описание
Resolving the nanometer-scale structure of biomolecules in natural conditions still remains a challenging task. We report the first distance measurement in nucleic acid at physiological temperature using electron paramagnetic resonance (EPR). The model 10-mer DNA duplex has been labeled with reactive forms of triarylmethyl radicals and then immobilized on a sorbent in water solution and investigated by double quantum coherence EPR. We succeeded in development of optimal triarylmethyl-based labels, approach for site-directed spin labeling and efficient immobilization procedure that, working together, allowed us to measure as long distances as ~4.6 nm with high accuracy at 310 K (37 °C).
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ГОСТ |
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Shevelev G. Y. et al. Physiological-Temperature Distance Measurement in Nucleic Acid using Triarylmethyl-Based Spin Labels and Pulsed Dipolar EPR Spectroscopy // Journal of the American Chemical Society. 2014. Vol. 136. No. 28. pp. 9874-9877.
ГОСТ со всеми авторами (до 50) Скопировать
Shevelev G. Y., Krumkacheva O. A., Ломзов А. А., Kuzhelev A. A., Tormyshev V. M., Fedin M., Пышный Д. В., Bagryanskaya E. G. Physiological-Temperature Distance Measurement in Nucleic Acid using Triarylmethyl-Based Spin Labels and Pulsed Dipolar EPR Spectroscopy // Journal of the American Chemical Society. 2014. Vol. 136. No. 28. pp. 9874-9877.
RIS |
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TY - JOUR
DO - 10.1021/ja505122n
UR - https://doi.org/10.1021/ja505122n
TI - Physiological-Temperature Distance Measurement in Nucleic Acid using Triarylmethyl-Based Spin Labels and Pulsed Dipolar EPR Spectroscopy
T2 - Journal of the American Chemical Society
AU - Shevelev, Georgiy Yu.
AU - Krumkacheva, Olesya A
AU - Ломзов, А. А.
AU - Kuzhelev, Andrey A
AU - Tormyshev, Victor M
AU - Fedin, Matvey
AU - Пышный, Д. В.
AU - Bagryanskaya, Elena G.
PY - 2014
DA - 2014/07/01
PB - American Chemical Society (ACS)
SP - 9874-9877
IS - 28
VL - 136
PMID - 24963806
SN - 0002-7863
SN - 1520-5126
ER -
BibTex |
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BibTex (до 50 авторов) Скопировать
@article{2014_Shevelev,
author = {Georgiy Yu. Shevelev and Olesya A Krumkacheva and А. А. Ломзов and Andrey A Kuzhelev and Victor M Tormyshev and Matvey Fedin and Д. В. Пышный and Elena G. Bagryanskaya},
title = {Physiological-Temperature Distance Measurement in Nucleic Acid using Triarylmethyl-Based Spin Labels and Pulsed Dipolar EPR Spectroscopy},
journal = {Journal of the American Chemical Society},
year = {2014},
volume = {136},
publisher = {American Chemical Society (ACS)},
month = {jul},
url = {https://doi.org/10.1021/ja505122n},
number = {28},
pages = {9874--9877},
doi = {10.1021/ja505122n}
}
MLA
Цитировать
Shevelev, Georgiy Yu., et al. “Physiological-Temperature Distance Measurement in Nucleic Acid using Triarylmethyl-Based Spin Labels and Pulsed Dipolar EPR Spectroscopy.” Journal of the American Chemical Society, vol. 136, no. 28, Jul. 2014, pp. 9874-9877. https://doi.org/10.1021/ja505122n.