Analytical Chemistry, volume 91, issue 16, pages 10448-10457
Aptamer-Based Fluorescent Sensor Array for Multiplexed Detection of Cyanotoxins on a Smartphone
Publication type: Journal Article
Publication date: 2019-06-13
Journal:
Analytical Chemistry
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 7.4
ISSN: 00032700, 15206882, 21542686
Analytical Chemistry
Abstract
Developing easy-to-use and miniaturized detectors is essential for in-field monitoring of environmentally hazardous substances, such as the cyanotoxins. We demonstrated a differential fluorescent sensor array made of aptamers and single-stranded DNA (ssDNA) dyes for multiplexed detection and discrimination of four common cyanotoxins with an ordinary smartphone within 5 min of reaction. The assay reagents were preloaded and dried in a microfluidic chip with a long shelf life over 60 days. Upon the addition of analyte solutions, competitive binding of cyanotoxin to the specific aptamer-dye conjugate occurred. A zone-specific and concentration-dependent reduction in the green fluorescence was observed as a result of the aptamer conformation change. The aptasensors are fully optimized by quantification of their dissociation constants, tuning the stoichiometric ratios of reaction mixtures, and implementation of an internal intensity correction step. The fluorescent sensor array allowed for accurate identification and measurement of four important cyanotoxins, including anatoxin-a (ATX), cylindrospermopsin (CYN), nodularin (NOD), and microcystin-LR (MC-LR), in parallel, with the limit of detection (LOD) down to a few nanomolar (<3 nM), which is close to the World Health Organization's guideline for the maximum concentration allowed in drinking water. The smartphone-based sensor platform also showed remarkable chemical specificity against potential interfering agents in water. The performance of the system was tested and validated with real lake water samples that were contaminated with trace levels of individual cyanotoxins as well as binary, ternary, and quaternary mixtures. Finally, a smartphone app interface has been developed for rapid on-site data processing and result display.
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Li Z. et al. Aptamer-Based Fluorescent Sensor Array for Multiplexed Detection of Cyanotoxins on a Smartphone // Analytical Chemistry. 2019. Vol. 91. No. 16. pp. 10448-10457.
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Li Z., Zhang S., Yu T., DAI Z., Wei Q. Aptamer-Based Fluorescent Sensor Array for Multiplexed Detection of Cyanotoxins on a Smartphone // Analytical Chemistry. 2019. Vol. 91. No. 16. pp. 10448-10457.
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TY - JOUR
DO - 10.1021/acs.analchem.9b00750
UR - https://doi.org/10.1021/acs.analchem.9b00750
TI - Aptamer-Based Fluorescent Sensor Array for Multiplexed Detection of Cyanotoxins on a Smartphone
T2 - Analytical Chemistry
AU - DAI, ZHIMING
AU - Li, Zheng
AU - Wei, Qingshan
AU - Zhang, Shengwei
AU - Yu, Tao
PY - 2019
DA - 2019/06/13 00:00:00
PB - American Chemical Society (ACS)
SP - 10448-10457
IS - 16
VL - 91
SN - 0003-2700
SN - 1520-6882
SN - 2154-2686
ER -
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@article{2019_Li,
author = {ZHIMING DAI and Zheng Li and Qingshan Wei and Shengwei Zhang and Tao Yu},
title = {Aptamer-Based Fluorescent Sensor Array for Multiplexed Detection of Cyanotoxins on a Smartphone},
journal = {Analytical Chemistry},
year = {2019},
volume = {91},
publisher = {American Chemical Society (ACS)},
month = {jun},
url = {https://doi.org/10.1021/acs.analchem.9b00750},
number = {16},
pages = {10448--10457},
doi = {10.1021/acs.analchem.9b00750}
}
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MLA
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Li, Zheng, et al. “Aptamer-Based Fluorescent Sensor Array for Multiplexed Detection of Cyanotoxins on a Smartphone.” Analytical Chemistry, vol. 91, no. 16, Jun. 2019, pp. 10448-10457. https://doi.org/10.1021/acs.analchem.9b00750.