ACS Applied Bio Materials, volume 2, issue 12, pages 5880-5887
Fluorescent Label-Free Aptasensor Integrated in a Lab-on-Chip System for the Detection of Ochratoxin A in Beer and Wheat
Francesca Costantini
1
,
Nicola Lovecchio
2
,
Albert Ruggi
3
,
Cesare Manetti
4
,
Augusto Nascetti
5
,
Massimo Reverberi
4
,
Giampiero de Cesare
2
,
Domenico Caputo
2
1
4
Publication type: Journal Article
Publication date: 2019-11-07
Journal:
ACS Applied Bio Materials
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 4.6
ISSN: 25766422, 25766422
General Chemistry
Biomaterials
Biomedical Engineering
Biochemistry (medical)
Abstract
This paper reports on the development of a fluorescent label-free aptamer assay integrated in a lab-on-chip (LoC) system for the detection of Ochratoxin A (OTA). The detection system relies on the integration, on a single glass substrate, of an array of amorphous silicon photosensors and a long pass interferential filter. The aptamer assay, integrated into the microfluidic network, is an aptasensor having affinity versus OTA, selected as a case study. The fluorescent molecule is a "light switch" complex [Ru(phen)2(dppz)]2+. The aptamer is directly anchored into a layer of poly(2-hydroxyethyl methacrylate) polymer brushes grown inside the channels. The fluorophore is intercalated between the base pairs of the aptamer. Upon the interaction of OTA with the aptasensor, a change of the aptamer conformation causes the release of the fluorophore, yielding a decrease of the fluorescent signal detected by the array of the amorphous silicon photosensors positioned underneath the microfluidic network. The developed LoC is a portable system capable of performing the analysis with a small volume of sample (about 10 μL) in a short time (5 min) with a limit of detection for OTA equal to 1.3 ng/mL. The LoC has been applied for the detection of OTA (5-200 ng/mL) in beer and wheat samples.
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Citations by publishers
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2
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12
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11 publications, 47.83%
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2 publications, 8.7%
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1 publication, 4.35%
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1 publication, 4.35%
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1 publication, 4.35%
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1 publication, 4.35%
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Costantini F. et al. Fluorescent Label-Free Aptasensor Integrated in a Lab-on-Chip System for the Detection of Ochratoxin A in Beer and Wheat // ACS Applied Bio Materials. 2019. Vol. 2. No. 12. pp. 5880-5887.
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Costantini F., Lovecchio N., Ruggi A., Manetti C., Nascetti A., Reverberi M., de Cesare G., Caputo D. Fluorescent Label-Free Aptasensor Integrated in a Lab-on-Chip System for the Detection of Ochratoxin A in Beer and Wheat // ACS Applied Bio Materials. 2019. Vol. 2. No. 12. pp. 5880-5887.
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TY - JOUR
DO - 10.1021/acsabm.9b00831
UR - https://doi.org/10.1021/acsabm.9b00831
TI - Fluorescent Label-Free Aptasensor Integrated in a Lab-on-Chip System for the Detection of Ochratoxin A in Beer and Wheat
T2 - ACS Applied Bio Materials
AU - Lovecchio, Nicola
AU - Ruggi, Albert
AU - Manetti, Cesare
AU - Nascetti, Augusto
AU - Reverberi, Massimo
AU - Caputo, Domenico
AU - Costantini, Francesca
AU - de Cesare, Giampiero
PY - 2019
DA - 2019/11/07 00:00:00
PB - American Chemical Society (ACS)
SP - 5880-5887
IS - 12
VL - 2
SN - 2576-6422
SN - 2576-6422
ER -
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@article{2019_Costantini,
author = {Nicola Lovecchio and Albert Ruggi and Cesare Manetti and Augusto Nascetti and Massimo Reverberi and Domenico Caputo and Francesca Costantini and Giampiero de Cesare},
title = {Fluorescent Label-Free Aptasensor Integrated in a Lab-on-Chip System for the Detection of Ochratoxin A in Beer and Wheat},
journal = {ACS Applied Bio Materials},
year = {2019},
volume = {2},
publisher = {American Chemical Society (ACS)},
month = {nov},
url = {https://doi.org/10.1021/acsabm.9b00831},
number = {12},
pages = {5880--5887},
doi = {10.1021/acsabm.9b00831}
}
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Costantini, Francesca, et al. “Fluorescent Label-Free Aptasensor Integrated in a Lab-on-Chip System for the Detection of Ochratoxin A in Beer and Wheat.” ACS Applied Bio Materials, vol. 2, no. 12, Nov. 2019, pp. 5880-5887. https://doi.org/10.1021/acsabm.9b00831.