Nature Structural Biology, volume 7, issue 4, pages 273-277

Crystal structure of the S15–rRNA complex

Dumas Philippe ^{1, 2}

Nikulin Alexei ^{1, 2}

Serganov Alexander ²

Ennifar Eric ¹

Tishchenko Svetlana ¹

Nevskaya Natalia ³

Shepard William ⁴

Portier Claude ¹

Garber Maria ²

Ehresmann Bernard ²

Ehresmann Chantal ¹

Nikonov Stanislav ²

Hide authors affiliations Show authors affiliations: 4 affiliations

Institute of Protein Research, Moscow Region, Russia |

UPR 9002 du CNRS, IBMC, Strasbourg cedex, France |

LURE, Centre Universitaire Paris-Sud, Orsay cedex

⁴

IBPC, Paris, France |

Publication type: Journal Article

Publication date: 2000-04-01

Springer Nature

Journal: Nature Structural Biology

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ISSN: 10728368

DOI: 10.1038/74028

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Biochemistry

Genetics

Structural Biology

Abstract

In bacterial ribosomes, the small (30S) ribosomal subunit is composed of 16S rRNA and 21 distinct proteins. Ribosomal protein S15 is of particular interest because it binds primarily to 16S rRNA and is required for assembly of the small subunit and for intersubunit association, thus representing a key element in the assembly of a whole ribosome. Here we report the 2.8 Å resolution crystal structure of the highly conserved S15–rRNA complex. Protein S15 interacts in the minor groove with a G-U/G-C motif and a three-way junction. The latter is constrained by a conserved base triple and stacking interactions, and locked into place by magnesium ions and protein side chains, mainly through interactions with the unique three-dimensional geometry of the backbone. The present structure gives insights into the dual role of S15 in ribosome assembly and translational regulation.

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Dumas P. et al. Crystal structure of the S15–rRNA complex // Nature Structural Biology. 2000. Vol. 7. No. 4. pp. 273-277.

GOST all authors (up to 50) Copy

Dumas P., Nikulin A., Serganov A., Ennifar E., Tishchenko S., Nevskaya N., Shepard W., Portier C., Garber M., Ehresmann B., Ehresmann C., Nikonov S. Crystal structure of the S15–rRNA complex // Nature Structural Biology. 2000. Vol. 7. No. 4. pp. 273-277.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1038/74028

UR - https://doi.org/10.1038%2F74028

TI - Crystal structure of the S15–rRNA complex

T2 - Nature Structural Biology

AU - Dumas, Philippe

AU - Nikulin, Alexei

AU - Serganov, Alexander

AU - Ennifar, Eric

AU - Tishchenko, Svetlana

AU - Nevskaya, Natalia

AU - Shepard, William

AU - Portier, Claude

AU - Garber, Maria

AU - Ehresmann, Bernard

AU - Ehresmann, Chantal

AU - Nikonov, Stanislav

PY - 2000

DA - 2000/04/01 00:00:00

PB - Springer Nature

SP - 273-277

IS - 4

VL - 7

SN - 1072-8368

ER -

BibTex |

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BibTex Copy

@article{2000_Dumas,

author = {Philippe Dumas and Alexei Nikulin and Alexander Serganov and Eric Ennifar and Svetlana Tishchenko and Natalia Nevskaya and William Shepard and Claude Portier and Maria Garber and Bernard Ehresmann and Chantal Ehresmann and Stanislav Nikonov},

title = {Crystal structure of the S15–rRNA complex},

journal = {Nature Structural Biology},

year = {2000},

volume = {7},

publisher = {Springer Nature},

month = {apr},

url = {https://doi.org/10.1038%2F74028},

number = {4},

pages = {273--277},

doi = {10.1038/74028}

}

MLA

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Dumas, Philippe, et al. “Crystal structure of the S15–rRNA complex.” Nature Structural Biology, vol. 7, no. 4, Apr. 2000, pp. 273-277. https://doi.org/10.1038%2F74028.

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Springer Nature

Journal

Nature Structural Biology

Quartile SCImago

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Quartile WOS

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ISSN

10728368 (Print)

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Laboratory of structural studies of the broadcasting apparatus

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