Crystal structure of the S15–rRNA complex
Philippe Dumas
1, 2
,
Alexei Nikulin
1, 2
,
Alexander Serganov
2
,
Eric Ennifar
1
,
Natalia Nevskaya
3
,
William Shepard
4
,
Claude Portier
1
,
Maria Garber
2
,
Bernard Ehresmann
2
,
Chantal Ehresmann
1
,
Stanislav Nikonov
2
2
UPR 9002 du CNRS, IBMC, Strasbourg cedex, France
|
3
LURE, Centre Universitaire Paris-Sud, Orsay cedex
4
IBPC, Paris, France
|
Publication type: Journal Article
Publication date: 2000-04-01
DOI:
10.1038/74028
PubMed ID:
10742169
Biochemistry
Genetics
Structural Biology
Abstract
In bacterial ribosomes, the small (30S) ribosomal subunit is composed of 16S rRNA and 21 distinct proteins. Ribosomal protein S15 is of particular interest because it binds primarily to 16S rRNA and is required for assembly of the small subunit and for intersubunit association, thus representing a key element in the assembly of a whole ribosome. Here we report the 2.8 Å resolution crystal structure of the highly conserved S15–rRNA complex. Protein S15 interacts in the minor groove with a G-U/G-C motif and a three-way junction. The latter is constrained by a conserved base triple and stacking interactions, and locked into place by magnesium ions and protein side chains, mainly through interactions with the unique three-dimensional geometry of the backbone. The present structure gives insights into the dual role of S15 in ribosome assembly and translational regulation.
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62
Total citations:
62
Citations from 2025:
1
(1.61%)
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GOST
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Dumas P. et al. Crystal structure of the S15–rRNA complex // Nature Structural Biology. 2000. Vol. 7. No. 4. pp. 273-277.
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Dumas P., Nikulin A., Serganov A., Ennifar E., Tishchenko S., Nevskaya N., Shepard W., Portier C., Garber M., Ehresmann B., Ehresmann C., Nikonov S. Crystal structure of the S15–rRNA complex // Nature Structural Biology. 2000. Vol. 7. No. 4. pp. 273-277.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1038/74028
UR - https://doi.org/10.1038/74028
TI - Crystal structure of the S15–rRNA complex
T2 - Nature Structural Biology
AU - Dumas, Philippe
AU - Nikulin, Alexei
AU - Serganov, Alexander
AU - Ennifar, Eric
AU - Tishchenko, Svetlana
AU - Nevskaya, Natalia
AU - Shepard, William
AU - Portier, Claude
AU - Garber, Maria
AU - Ehresmann, Bernard
AU - Ehresmann, Chantal
AU - Nikonov, Stanislav
PY - 2000
DA - 2000/04/01
PB - Springer Nature
SP - 273-277
IS - 4
VL - 7
PMID - 10742169
SN - 1072-8368
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2000_Dumas,
author = {Philippe Dumas and Alexei Nikulin and Alexander Serganov and Eric Ennifar and Svetlana Tishchenko and Natalia Nevskaya and William Shepard and Claude Portier and Maria Garber and Bernard Ehresmann and Chantal Ehresmann and Stanislav Nikonov},
title = {Crystal structure of the S15–rRNA complex},
journal = {Nature Structural Biology},
year = {2000},
volume = {7},
publisher = {Springer Nature},
month = {apr},
url = {https://doi.org/10.1038/74028},
number = {4},
pages = {273--277},
doi = {10.1038/74028}
}
Cite this
MLA
Copy
Dumas, Philippe, et al. “Crystal structure of the S15–rRNA complex.” Nature Structural Biology, vol. 7, no. 4, Apr. 2000, pp. 273-277. https://doi.org/10.1038/74028.