Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy
Francesca Pennacchietti
1
,
Aline R Faro
3
,
Irina I Shemyakina
2
,
Nina G Bozhanova
2
,
Alexey A Kotlobay
2
,
Andreas Bodén
1
,
Jes Dreier
1
,
Dmitry M. CHUDAKOV
2
,
Konstantin A. Lukyanov
2
,
Vladislav V. Verkhusha
3
,
Publication type: Journal Article
Publication date: 2018-07-09
scimago Q1
wos Q1
SJR: 17.251
CiteScore: 49.0
Impact factor: 32.1
ISSN: 15487091, 15487105
PubMed ID:
29988095
Biochemistry
Molecular Biology
Cell Biology
Biotechnology
Abstract
Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells. Bright reversibly switching red fluorescent proteins (rsFusionReds) with fast switching kinetics and low fatigue enable RESOLFT and MoNaLISA nanoscopy of live cells with green-orange illumination, which further reduces the risk of phototoxicity.
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Total citations:
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Citations from 2024:
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GOST
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Pennacchietti F. et al. Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy // Nature Methods. 2018. Vol. 15. No. 8. pp. 601-604.
GOST all authors (up to 50)
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Pennacchietti F., Serebrovskaya E. O., Faro A. R., Shemyakina I. I., Bozhanova N. G., Kotlobay A. A., GURSKAYA N. G., Bodén A., Dreier J., CHUDAKOV D. M., Lukyanov K. A., Verkhusha V. V., Mishin A. S., Testa I. Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy // Nature Methods. 2018. Vol. 15. No. 8. pp. 601-604.
Cite this
RIS
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TY - JOUR
DO - 10.1038/s41592-018-0052-9
UR - https://doi.org/10.1038/s41592-018-0052-9
TI - Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy
T2 - Nature Methods
AU - Pennacchietti, Francesca
AU - Serebrovskaya, Ekaterina O.
AU - Faro, Aline R
AU - Shemyakina, Irina I
AU - Bozhanova, Nina G
AU - Kotlobay, Alexey A
AU - GURSKAYA, Nadya G.
AU - Bodén, Andreas
AU - Dreier, Jes
AU - CHUDAKOV, Dmitry M.
AU - Lukyanov, Konstantin A.
AU - Verkhusha, Vladislav V.
AU - Mishin, Alexander S.
AU - Testa, Ilaria
PY - 2018
DA - 2018/07/09
PB - Springer Nature
SP - 601-604
IS - 8
VL - 15
PMID - 29988095
SN - 1548-7091
SN - 1548-7105
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2018_Pennacchietti,
author = {Francesca Pennacchietti and Ekaterina O. Serebrovskaya and Aline R Faro and Irina I Shemyakina and Nina G Bozhanova and Alexey A Kotlobay and Nadya G. GURSKAYA and Andreas Bodén and Jes Dreier and Dmitry M. CHUDAKOV and Konstantin A. Lukyanov and Vladislav V. Verkhusha and Alexander S. Mishin and Ilaria Testa},
title = {Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy},
journal = {Nature Methods},
year = {2018},
volume = {15},
publisher = {Springer Nature},
month = {jul},
url = {https://doi.org/10.1038/s41592-018-0052-9},
number = {8},
pages = {601--604},
doi = {10.1038/s41592-018-0052-9}
}
Cite this
MLA
Copy
Pennacchietti, Francesca, et al. “Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy.” Nature Methods, vol. 15, no. 8, Jul. 2018, pp. 601-604. https://doi.org/10.1038/s41592-018-0052-9.