Nature Methods, volume 15, issue 8, pages 601-604
Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy
Pennacchietti Francesca
1
,
Faro Aline R
3
,
Shemyakina Irina I
2
,
Bozhanova Nina G
2
,
Kotlobay Alexey A
2
,
Bodén Andreas
1
,
Dreier Jes
1
,
CHUDAKOV Dmitry M.
2
,
Lukyanov Konstantin A.
2
,
Verkhusha Vladislav V.
3
,
3
Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, USA
|
Publication type: Journal Article
Publication date: 2018-07-09
Journal:
Nature Methods
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 48
ISSN: 15487091, 15487105
Biochemistry
Molecular Biology
Cell Biology
Biotechnology
Abstract
Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells. Bright reversibly switching red fluorescent proteins (rsFusionReds) with fast switching kinetics and low fatigue enable RESOLFT and MoNaLISA nanoscopy of live cells with green-orange illumination, which further reduces the risk of phototoxicity.
Citations by journals
Citations by publishers
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10
12
14
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Springer Nature
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Springer Nature
13 publications, 18.57%
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Elsevier
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Elsevier
13 publications, 18.57%
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American Chemical Society (ACS)
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American Chemical Society (ACS)
10 publications, 14.29%
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Royal Society of Chemistry (RSC)
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Royal Society of Chemistry (RSC)
6 publications, 8.57%
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Wiley
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Wiley
5 publications, 7.14%
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
4 publications, 5.71%
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IOP Publishing
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IOP Publishing
3 publications, 4.29%
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The Chemical Society of Japan
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The Chemical Society of Japan
1 publication, 1.43%
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Pleiades Publishing
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Pleiades Publishing
1 publication, 1.43%
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Oxford University Press
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Oxford University Press
1 publication, 1.43%
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Optical Society of America
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Optical Society of America
1 publication, 1.43%
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American Society for Cell Biology (ASCB)
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American Society for Cell Biology (ASCB)
1 publication, 1.43%
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SPIE
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SPIE
1 publication, 1.43%
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2
4
6
8
10
12
14
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Pennacchietti F. et al. Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy // Nature Methods. 2018. Vol. 15. No. 8. pp. 601-604.
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Pennacchietti F., Serebrovskaya E. O., Faro A. R., Shemyakina I. I., Bozhanova N. G., Kotlobay A. A., GURSKAYA N. G., Bodén A., Dreier J., CHUDAKOV D. M., Lukyanov K. A., Verkhusha V. V., Mishin A. S., Testa I. Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy // Nature Methods. 2018. Vol. 15. No. 8. pp. 601-604.
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TY - JOUR
DO - 10.1038/s41592-018-0052-9
UR - https://doi.org/10.1038%2Fs41592-018-0052-9
TI - Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy
T2 - Nature Methods
AU - Pennacchietti, Francesca
AU - Serebrovskaya, Ekaterina O.
AU - Faro, Aline R
AU - Shemyakina, Irina I
AU - Bozhanova, Nina G
AU - Kotlobay, Alexey A
AU - Bodén, Andreas
AU - Dreier, Jes
AU - CHUDAKOV, Dmitry M.
AU - Lukyanov, Konstantin A.
AU - Verkhusha, Vladislav V.
AU - Mishin, Alexander S.
AU - Testa, Ilaria
AU - GURSKAYA, Nadya G.
PY - 2018
DA - 2018/07/09 00:00:00
PB - Springer Nature
SP - 601-604
IS - 8
VL - 15
SN - 1548-7091
SN - 1548-7105
ER -
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@article{2018_Pennacchietti,
author = {Francesca Pennacchietti and Ekaterina O. Serebrovskaya and Aline R Faro and Irina I Shemyakina and Nina G Bozhanova and Alexey A Kotlobay and Andreas Bodén and Jes Dreier and Dmitry M. CHUDAKOV and Konstantin A. Lukyanov and Vladislav V. Verkhusha and Alexander S. Mishin and Ilaria Testa and Nadya G. GURSKAYA},
title = {Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy},
journal = {Nature Methods},
year = {2018},
volume = {15},
publisher = {Springer Nature},
month = {jul},
url = {https://doi.org/10.1038%2Fs41592-018-0052-9},
number = {8},
pages = {601--604},
doi = {10.1038/s41592-018-0052-9}
}
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Pennacchietti, Francesca, et al. “Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy.” Nature Methods, vol. 15, no. 8, Jul. 2018, pp. 601-604. https://doi.org/10.1038%2Fs41592-018-0052-9.