Nature Methods

, том 15 , издание 8 , страницы 601-604

Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy

Тип публикации: Journal Article

Дата публикации: 2018-07-09

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Nature Methods

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SJR: 15.624

CiteScore: 40

Impact factor: 28.3

ISSN: 15487091, 15487105

DOI: 10.1038/s41592-018-0052-9

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PubMed ID: 29988095

Biochemistry

Molecular Biology

Cell Biology

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Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells. Bright reversibly switching red fluorescent proteins (rsFusionReds) with fast switching kinetics and low fatigue enable RESOLFT and MoNaLISA nanoscopy of live cells with green-orange illumination, which further reduces the risk of phototoxicity.

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Pennacchietti F. et al. Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy // Nature Methods. 2018. Vol. 15. No. 8. pp. 601-604.

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Pennacchietti F., Serebrovskaya E. O., Faro A. R., Shemyakina I. I., Bozhanova N. G., Kotlobay A. A., GURSKAYA N. G., Bodén A., Dreier J., CHUDAKOV D. M., Lukyanov K. A., Verkhusha V. V., Mishin A. S., Testa I. Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy // Nature Methods. 2018. Vol. 15. No. 8. pp. 601-604.

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TY - JOUR

DO - 10.1038/s41592-018-0052-9

UR - https://doi.org/10.1038/s41592-018-0052-9

TI - Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy

T2 - Nature Methods

AU - Pennacchietti, Francesca

AU - Serebrovskaya, Ekaterina O.

AU - Faro, Aline R

AU - Shemyakina, Irina I

AU - Bozhanova, Nina G

AU - Kotlobay, Alexey A

AU - GURSKAYA, Nadya G.

AU - Bodén, Andreas

AU - Dreier, Jes

AU - CHUDAKOV, Dmitry M.

AU - Lukyanov, Konstantin A.

AU - Verkhusha, Vladislav V.

AU - Mishin, Alexander S.

AU - Testa, Ilaria

PY - 2018

DA - 2018/07/09

PB - Springer Nature

SP - 601-604

IS - 8

VL - 15

PMID - 29988095

SN - 1548-7091

SN - 1548-7105

ER -

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@article{2018_Pennacchietti,

author = {Francesca Pennacchietti and Ekaterina O. Serebrovskaya and Aline R Faro and Irina I Shemyakina and Nina G Bozhanova and Alexey A Kotlobay and Nadya G. GURSKAYA and Andreas Bodén and Jes Dreier and Dmitry M. CHUDAKOV and Konstantin A. Lukyanov and Vladislav V. Verkhusha and Alexander S. Mishin and Ilaria Testa},

title = {Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy},

journal = {Nature Methods},

year = {2018},

volume = {15},

publisher = {Springer Nature},

month = {jul},

url = {https://doi.org/10.1038/s41592-018-0052-9},

number = {8},

pages = {601--604},

doi = {10.1038/s41592-018-0052-9}

}

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Pennacchietti, Francesca, et al. “Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy.” Nature Methods, vol. 15, no. 8, Jul. 2018, pp. 601-604. https://doi.org/10.1038/s41592-018-0052-9.

Издатель

Springer Nature

Журнал

Nature Methods

SCImago Q1

Tоп 10% SCImago

WOS Q1

БС1