Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances
Publication type: Journal Article
Publication date: 2008-01-17
scimago Q1
wos Q1
SJR: 5.854
CiteScore: 27.6
Impact factor: 16.0
ISSN: 17542189, 17502799
PubMed ID:
18274517
General Biochemistry, Genetics and Molecular Biology
Abstract
The aim of broth and agar dilution methods is to determine the lowest concentration of the assayed antimicrobial agent (minimal inhibitory concentration, MIC) that, under defined test conditions, inhibits the visible growth of the bacterium being investigated. MIC values are used to determine susceptibilities of bacteria to drugs and also to evaluate the activity of new antimicrobial agents. Agar dilution involves the incorporation of different concentrations of the antimicrobial substance into a nutrient agar medium followed by the application of a standardized number of cells to the surface of the agar plate. For broth dilution, often determined in 96-well microtiter plate format, bacteria are inoculated into a liquid growth medium in the presence of different concentrations of an antimicrobial agent. Growth is assessed after incubation for a defined period of time (16–20 h) and the MIC value is read. This protocol applies only to aerobic bacteria and can be completed in 3 d.
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GOST
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Wiegand I., Hilpert K., Hancock R. E. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances // Nature Protocols. 2008. Vol. 3. No. 2. pp. 163-175.
GOST all authors (up to 50)
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Wiegand I., Hilpert K., Hancock R. E. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances // Nature Protocols. 2008. Vol. 3. No. 2. pp. 163-175.
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RIS
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TY - JOUR
DO - 10.1038/nprot.2007.521
UR - https://doi.org/10.1038/nprot.2007.521
TI - Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances
T2 - Nature Protocols
AU - Wiegand, Irith
AU - Hilpert, Kai
AU - Hancock, Robert E.W.
PY - 2008
DA - 2008/01/17
PB - Springer Nature
SP - 163-175
IS - 2
VL - 3
PMID - 18274517
SN - 1754-2189
SN - 1750-2799
ER -
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BibTex (up to 50 authors)
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@article{2008_Wiegand,
author = {Irith Wiegand and Kai Hilpert and Robert E.W. Hancock},
title = {Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances},
journal = {Nature Protocols},
year = {2008},
volume = {3},
publisher = {Springer Nature},
month = {jan},
url = {https://doi.org/10.1038/nprot.2007.521},
number = {2},
pages = {163--175},
doi = {10.1038/nprot.2007.521}
}
Cite this
MLA
Copy
Wiegand, Irith, et al. “Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances.” Nature Protocols, vol. 3, no. 2, Jan. 2008, pp. 163-175. https://doi.org/10.1038/nprot.2007.521.