Nature Methods

, том 15 , издание 8 , страницы 601-604

Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy

Тип публикации: Journal Article

Дата публикации: 2018-07-09

Springer Nature

Nature Methods

scimago Q1

wos Q1

БС1

SJR: 17.251

CiteScore: 49.0

Impact factor: 32.1

ISSN: 15487091, 15487105

DOI: 10.1038/s41592-018-0052-9

Скопировать DOI

PubMed ID: 29988095

Biochemistry

Molecular Biology

Cell Biology

Biotechnology

Краткое описание

Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells. Bright reversibly switching red fluorescent proteins (rsFusionReds) with fast switching kinetics and low fatigue enable RESOLFT and MoNaLISA nanoscopy of live cells with green-orange illumination, which further reduces the risk of phototoxicity.

Найдено

2 цитирования

Мишин Александр

к.б.н.

83 публикации, 2 832 цитирования, 9 рецензий

Индекс Хирша: 26

Институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова РАН

1 цитирование

Гурская Надя

60 публикаций, 5 450 цитирований, 13 рецензий

Индекс Хирша: 24

Российский национальный исследовательский медицинский университет имени Н. И. Пирогова

1 цитирование

Бойко Константин

🥼 🤝

к.б.н.

129 публикаций, 1 324 цитирования, 1 рецензия

Индекс Хирша: 22

ФИЦ «Фундаментальные Основы Биотехнологии» РАН

Области научных интересов

Биоинформатика

Структурная биология

1 цитирование

Ручкин Дмитрий

6 публикаций, 71 цитирование

Индекс Хирша: 3

Институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова РАН

Топ-30

Журналы

	1 2 3 4
Nature Communications	Nature Communications, 4, 4.4% Nature Communications 4 публикации, 4.4%
Nature Methods	Nature Methods, 4, 4.4% Nature Methods 4 публикации, 4.4%
Nanoscale	Nanoscale, 3, 3.3% Nanoscale 3 публикации, 3.3%
Journal of the American Chemical Society	Journal of the American Chemical Society, 2, 2.2% Journal of the American Chemical Society 2 публикации, 2.2%
Chemical Society Reviews	Chemical Society Reviews, 2, 2.2% Chemical Society Reviews 2 публикации, 2.2%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 2, 2.2% International Journal of Molecular Sciences 2 публикации, 2.2%
Biosensors	Biosensors, 2, 2.2% Biosensors 2 публикации, 2.2%
International Journal of Biological Macromolecules	International Journal of Biological Macromolecules, 2, 2.2% International Journal of Biological Macromolecules 2 публикации, 2.2%
Journal Physics D: Applied Physics	Journal Physics D: Applied Physics, 2, 2.2% Journal Physics D: Applied Physics 2 публикации, 2.2%
Angewandte Chemie	Angewandte Chemie, 2, 2.2% Angewandte Chemie 2 публикации, 2.2%
Angewandte Chemie - International Edition	Angewandte Chemie - International Edition, 2, 2.2% Angewandte Chemie - International Edition 2 публикации, 2.2%
Biochemistry	Biochemistry, 2, 2.2% Biochemistry 2 публикации, 2.2%
Chemical Reviews	Chemical Reviews, 2, 2.2% Chemical Reviews 2 публикации, 2.2%
Biochemical and Biophysical Research Communications	Biochemical and Biophysical Research Communications, 2, 2.2% Biochemical and Biophysical Research Communications 2 публикации, 2.2%
Scientific Reports	Scientific Reports, 1, 1.1% Scientific Reports 1 публикация, 1.1%
Bulletin of the Chemical Society of Japan	Bulletin of the Chemical Society of Japan, 1, 1.1% Bulletin of the Chemical Society of Japan 1 публикация, 1.1%
Materials	Materials, 1, 1.1% Materials 1 публикация, 1.1%
Nature Biotechnology	Nature Biotechnology, 1, 1.1% Nature Biotechnology 1 публикация, 1.1%
Nature Reviews Neuroscience	Nature Reviews Neuroscience, 1, 1.1% Nature Reviews Neuroscience 1 публикация, 1.1%
Photochemical and Photobiological Sciences	Photochemical and Photobiological Sciences, 1, 1.1% Photochemical and Photobiological Sciences 1 публикация, 1.1%
Fundamental Research	Fundamental Research, 1, 1.1% Fundamental Research 1 публикация, 1.1%
FEBS Letters	FEBS Letters, 1, 1.1% FEBS Letters 1 публикация, 1.1%
Methods and Applications in Fluorescence	Methods and Applications in Fluorescence, 1, 1.1% Methods and Applications in Fluorescence 1 публикация, 1.1%
Current Opinion in Biotechnology	Current Opinion in Biotechnology, 1, 1.1% Current Opinion in Biotechnology 1 публикация, 1.1%
Current Opinion in Biomedical Engineering	Current Opinion in Biomedical Engineering, 1, 1.1% Current Opinion in Biomedical Engineering 1 публикация, 1.1%
Cell Chemical Biology	Cell Chemical Biology, 1, 1.1% Cell Chemical Biology 1 публикация, 1.1%
Methods	Methods, 1, 1.1% Methods 1 публикация, 1.1%
Data in Brief	Data in Brief, 1, 1.1% Data in Brief 1 публикация, 1.1%
Neurobiology of Disease	Neurobiology of Disease, 1, 1.1% Neurobiology of Disease 1 публикация, 1.1%
	1 2 3 4

Издатели

	2 4 6 8 10 12 14 16
Springer Nature	Springer Nature, 16, 17.58% Springer Nature 16 публикаций, 17.58%
Elsevier	Elsevier, 16, 17.58% Elsevier 16 публикаций, 17.58%
American Chemical Society (ACS)	American Chemical Society (ACS), 11, 12.09% American Chemical Society (ACS) 11 публикаций, 12.09%
Cold Spring Harbor Laboratory	Cold Spring Harbor Laboratory, 11, 12.09% Cold Spring Harbor Laboratory 11 публикаций, 12.09%
Wiley	Wiley, 9, 9.89% Wiley 9 публикаций, 9.89%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 8, 8.79% Royal Society of Chemistry (RSC) 8 публикаций, 8.79%
MDPI	MDPI, 6, 6.59% MDPI 6 публикаций, 6.59%
IOP Publishing	IOP Publishing, 3, 3.3% IOP Publishing 3 публикации, 3.3%
Oxford University Press	Oxford University Press, 2, 2.2% Oxford University Press 2 публикации, 2.2%
Pleiades Publishing	Pleiades Publishing, 1, 1.1% Pleiades Publishing 1 публикация, 1.1%
Optica Publishing Group	Optica Publishing Group, 1, 1.1% Optica Publishing Group 1 публикация, 1.1%
Institute of Electrical and Electronics Engineers (IEEE)	Institute of Electrical and Electronics Engineers (IEEE), 1, 1.1% Institute of Electrical and Electronics Engineers (IEEE) 1 публикация, 1.1%
American Society for Cell Biology (ASCB)	American Society for Cell Biology (ASCB), 1, 1.1% American Society for Cell Biology (ASCB) 1 публикация, 1.1%
SPIE-Intl Soc Optical Eng	SPIE-Intl Soc Optical Eng, 1, 1.1% SPIE-Intl Soc Optical Eng 1 публикация, 1.1%
American Physical Society (APS)	American Physical Society (APS), 1, 1.1% American Physical Society (APS) 1 публикация, 1.1%
Moscow University Press	Moscow University Press, 1, 1.1% Moscow University Press 1 публикация, 1.1%
Allerton Press	Allerton Press, 1, 1.1% Allerton Press 1 публикация, 1.1%
International Union of Crystallography (IUCr)	International Union of Crystallography (IUCr), 1, 1.1% International Union of Crystallography (IUCr) 1 публикация, 1.1%
	2 4 6 8 10 12 14 16

Мы не учитываем публикации, у которых нет DOI.
Статистика публикаций обновляется еженедельно.

Вы ученый?

Создайте профиль, чтобы получать персональные рекомендации коллег, конференций и новых статей.

Войти с ORCID

Метрики

Цитировать

ГОСТ |

Цитировать

ГОСТ Скопировать

Pennacchietti F. et al. Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy // Nature Methods. 2018. Vol. 15. No. 8. pp. 601-604.

ГОСТ со всеми авторами (до 50) Скопировать

Pennacchietti F., Serebrovskaya E. O., Faro A. R., Shemyakina I. I., Bozhanova N. G., Kotlobay A. A., GURSKAYA N. G., Bodén A., Dreier J., CHUDAKOV D. M., Lukyanov K. A., Verkhusha V. V., Mishin A. S., Testa I. Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy // Nature Methods. 2018. Vol. 15. No. 8. pp. 601-604.

RIS |

Цитировать

RIS Скопировать

TY - JOUR

DO - 10.1038/s41592-018-0052-9

UR - https://doi.org/10.1038/s41592-018-0052-9

TI - Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy

T2 - Nature Methods

AU - Pennacchietti, Francesca

AU - Serebrovskaya, Ekaterina O.

AU - Faro, Aline R

AU - Shemyakina, Irina I

AU - Bozhanova, Nina G

AU - Kotlobay, Alexey A

AU - GURSKAYA, Nadya G.

AU - Bodén, Andreas

AU - Dreier, Jes

AU - CHUDAKOV, Dmitry M.

AU - Lukyanov, Konstantin A.

AU - Verkhusha, Vladislav V.

AU - Mishin, Alexander S.

AU - Testa, Ilaria

PY - 2018

DA - 2018/07/09

PB - Springer Nature

SP - 601-604

IS - 8

VL - 15

PMID - 29988095

SN - 1548-7091

SN - 1548-7105

ER -

BibTex |

Цитировать

BibTex (до 50 авторов) Скопировать

@article{2018_Pennacchietti,

author = {Francesca Pennacchietti and Ekaterina O. Serebrovskaya and Aline R Faro and Irina I Shemyakina and Nina G Bozhanova and Alexey A Kotlobay and Nadya G. GURSKAYA and Andreas Bodén and Jes Dreier and Dmitry M. CHUDAKOV and Konstantin A. Lukyanov and Vladislav V. Verkhusha and Alexander S. Mishin and Ilaria Testa},

title = {Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy},

journal = {Nature Methods},

year = {2018},

volume = {15},

publisher = {Springer Nature},

month = {jul},

url = {https://doi.org/10.1038/s41592-018-0052-9},

number = {8},

pages = {601--604},

doi = {10.1038/s41592-018-0052-9}

}

MLA

Цитировать

MLA Скопировать

Pennacchietti, Francesca, et al. “Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy.” Nature Methods, vol. 15, no. 8, Jul. 2018, pp. 601-604. https://doi.org/10.1038/s41592-018-0052-9.