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Scientific Reports, том 10, издание 1, номер публикации: 11109

Insights into the structure and function of Est3 from the Hansenula polymorpha telomerase

Тип документаJournal Article
Дата публикации2020-07-06
ИздательSpringer Nature
Название журналаScientific Reports
Квартиль по SCImagoQ1
Квартиль по Web of ScienceQ2
Импакт-фактор 20215.00
Краткое описание
Telomerase is a ribonucleoprotein enzyme, which maintains genome integrity in eukaryotes and ensures continuous cellular proliferation. Telomerase holoenzyme from the thermotolerant yeast Hansenula polymorpha, in addition to the catalytic subunit (TERT) and telomerase RNA (TER), contains accessory proteins Est1 and Est3, which are essential for in vivo telomerase function. Here we report the high-resolution structure of Est3 from Hansenula polymorpha (HpEst3) in solution, as well as the characterization of its functional relationships with other components of telomerase. The overall structure of HpEst3 is similar to that of Est3 from Saccharomyces cerevisiae and human TPP1. We have shown that telomerase activity in H. polymorpha relies on both Est3 and Est1 proteins in a functionally symmetrical manner. The absence of either Est3 or Est1 prevents formation of a stable ribonucleoprotein complex, weakens binding of a second protein to TER, and decreases the amount of cellular TERT, presumably due to the destabilization of telomerase RNP. NMR probing has shown no direct in vitro interactions of free Est3 either with the N-terminal domain of TERT or with DNA or RNA fragments mimicking the probable telomerase environment. Our findings corroborate the idea that telomerase possesses the evolutionarily variable functionality within the conservative structural context.
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1. Shepelev N.M. и др. Insights into the structure and function of Est3 from the Hansenula polymorpha telomerase // Scientific Reports. 2020. Т. 10. № 1.


DO - 10.1038/s41598-020-68107-x

UR -

TI - Insights into the structure and function of Est3 from the Hansenula polymorpha telomerase

T2 - Scientific Reports

AU - Shepelev, Nikita M.

AU - Mariasina, Sofia S.

AU - Mantsyzov, Alexey B.

AU - Malyavko, Alexander N.

AU - Efimov, Sergey V.

AU - Petrova, Olga A.

AU - Rodina, Elena V.

AU - Zvereva, Maria I.

AU - Dontsova, Olga A.

AU - Polshakov, Vladimir I.

PY - 2020

DA - 2020/07/06

PB - Springer Science and Business Media LLC

IS - 1

VL - 10

SN - 2045-2322

ER -

BibTex |


doi = {10.1038/s41598-020-68107-x},

url = {},

year = 2020,

month = {jul},

publisher = {Springer Science and Business Media {LLC}},

volume = {10},

number = {1},

author = {Nikita M. Shepelev and Sofia S. Mariasina and Alexey B. Mantsyzov and Alexander N. Malyavko and Sergey V. Efimov and Olga A. Petrova and Elena V. Rodina and Maria I. Zvereva and Olga A. Dontsova and Vladimir I. Polshakov},

title = {Insights into the structure and function of Est3 from the Hansenula polymorpha telomerase}


Shepelev, Nikita M. et al. “Insights into the Structure and Function of Est3 from the Hansenula Polymorpha Telomerase.” Scientific Reports 10.1 (2020): n. pag. Crossref. Web.