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Scientific Reports, volume 12, issue 1, publication number 18975

Single-nucleus transcriptomics of IDH1- and TP53-mutant glioma stem cells displays diversified commitment on invasive cancer progenitors

Gulaia Valeriia ¹

Shmelev Mikhail ¹

Romanishin Aleksander ^{1, 2}

Shved Nikita ^{1, 3}

Farniev Vladislav ¹

Goncharov Nikolay ¹

Biktimirov Arthur ¹

Vargas Irene Lisa ⁴

Khodosevich Konstantin ⁴

Kagansky Alexander ¹

Kumeiko Vadim ^{1, 3}

Hide authors affiliations Show authors affiliations: 4 affiliations

Institute of Life Sciences and Biomedicine, Medical Center, Far Eastern Federal University, Vladivostok, Russia |

School of Life Sciences, Immanuel Kant Baltic Federal University, Kaliningrad, Russia |

A.V. Zhirmunsky National Scientific Center of Marine Biology, FEB RAS, Vladivostok, Russia |

⁴

Biotech Research & Innovation Centre (BRIC), The Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark |

Publication type: Journal Article

Publication date: 2022-11-08

Springer Nature

Journal: Scientific Reports

Quartile SCImago

Quartile WOS

Impact factor: 4.6

ISSN: 20452322

DOI: 10.1038/s41598-022-23646-3

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Multidisciplinary

Abstract

Glioma is a devastating brain tumor with a high mortality rate attributed to the glioma stem cells (GSCs) possessing high plasticity. Marker mutations in isocitrate dehydrogenase type 1 (IDH1) and tumor protein 53 (TP53) are frequent in gliomas and impact the cell fate decisions. Understanding the GSC heterogeneity within IDH1- and TP53- mutant tumors may elucidate possible treatment targets. Here, we performed single-nucleus transcriptomics of mutant and wild-type glioma samples sorted for Sox2 stem cell marker. For the first time the rare subpopulations of Sox2 + IDH1- and TP53-mutant GSCs were characterized. In general, GSCs contained the heterogeneity root subpopulation resembling active neural stem cells capable of asymmetric division to quiescent and transit amplifying cell branches. Specifically, double-mutant GSCs revealed the commitment on highly invasive oligodendrocyte- and astroglia-like progenitors. Additionally, double-mutant GSCs displayed upregulated markers of collagen synthesis, altered lipogenesis and high migration, while wild-type GSCs expressed genes related to ATP production. Wild-type GSC root population was highly heterogeneous and lacked the signature marker expression, thus glioblastoma treatment should emphasize on establishing differentiation protocol directed against residual GSCs. For the more differentiated IDH1- and TP53-mutant gliomas we suggest therapeutic targeting of migration molecules, such as CD44.

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Citations by journals

	1
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 1, 11.11% International Journal of Molecular Sciences 1 publication, 11.11%
Frontiers in Neurology	Frontiers in Neurology, 1, 11.11% Frontiers in Neurology 1 publication, 11.11%
SSRN Electronic Journal	SSRN Electronic Journal, 1, 11.11% SSRN Electronic Journal 1 publication, 11.11%
Frontiers in Molecular Biosciences	Frontiers in Molecular Biosciences, 1, 11.11% Frontiers in Molecular Biosciences 1 publication, 11.11%
Cancers	Cancers, 1, 11.11% Cancers 1 publication, 11.11%
Cells	Cells, 1, 11.11% Cells 1 publication, 11.11%
Virology	Virology, 1, 11.11% Virology 1 publication, 11.11%
	1

Citations by publishers

	1 2 3
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 3, 33.33% Multidisciplinary Digital Publishing Institute (MDPI) 3 publications, 33.33%
Frontiers Media S.A.	Frontiers Media S.A., 2, 22.22% Frontiers Media S.A. 2 publications, 22.22%
Social Science Electronic Publishing	Social Science Electronic Publishing, 1, 11.11% Social Science Electronic Publishing 1 publication, 11.11%
Elsevier	Elsevier, 1, 11.11% Elsevier 1 publication, 11.11%
	1 2 3

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Gulaia V. et al. Single-nucleus transcriptomics of IDH1- and TP53-mutant glioma stem cells displays diversified commitment on invasive cancer progenitors // Scientific Reports. 2022. Vol. 12. No. 1. 18975

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Gulaia V., Shmelev M., Romanishin A., Shved N., Farniev V., Goncharov N., Biktimirov A., Vargas I. L., Khodosevich K., Kagansky A., Kumeiko V. Single-nucleus transcriptomics of IDH1- and TP53-mutant glioma stem cells displays diversified commitment on invasive cancer progenitors // Scientific Reports. 2022. Vol. 12. No. 1. 18975

RIS |

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RIS Copy

TY - JOUR

DO - 10.1038/s41598-022-23646-3

UR - https://doi.org/10.1038%2Fs41598-022-23646-3

TI - Single-nucleus transcriptomics of IDH1- and TP53-mutant glioma stem cells displays diversified commitment on invasive cancer progenitors

T2 - Scientific Reports

AU - Gulaia, Valeriia

AU - Shmelev, Mikhail

AU - Romanishin, Aleksander

AU - Shved, Nikita

AU - Farniev, Vladislav

AU - Goncharov, Nikolay

AU - Biktimirov, Arthur

AU - Vargas, Irene Lisa

AU - Khodosevich, Konstantin

AU - Kagansky, Alexander

AU - Kumeiko, Vadim

PY - 2022

DA - 2022/11/08 00:00:00

PB - Springer Nature

IS - 1

VL - 12

SN - 2045-2322

ER -

BibTex

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@article{2022_Gulaia,

author = {Valeriia Gulaia and Mikhail Shmelev and Aleksander Romanishin and Nikita Shved and Vladislav Farniev and Nikolay Goncharov and Arthur Biktimirov and Irene Lisa Vargas and Konstantin Khodosevich and Alexander Kagansky and Vadim Kumeiko},

title = {Single-nucleus transcriptomics of IDH1- and TP53-mutant glioma stem cells displays diversified commitment on invasive cancer progenitors},

journal = {Scientific Reports},

year = {2022},

volume = {12},

publisher = {Springer Nature},

month = {nov},

url = {https://doi.org/10.1038%2Fs41598-022-23646-3},

number = {1},

doi = {10.1038/s41598-022-23646-3}

}

Found error?

Publisher

Springer Nature

Journal

Scientific Reports

Quartile SCImago

Quartile WOS

Impact factor

4.6

ISSN

20452322 (Print)

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Биологи нашли потенциальные мишени для персонифицированной терапии глиомы