Open Access
NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids
Konstantin S. Mineev
1, 2, 3, 4, 5, 6, 7
,
Sergey A Goncharuk
1, 2, 3, 4, 5, 6, 7
,
Marina V Goncharuk
1
,
Natalia V Povarova
1, 3, 4, 5, 6
,
Anatolii I Sokolov
1, 3, 4, 5, 6
,
Alexander Yu Smirnov
1, 3, 4, 5, 6
,
I. N. Myasnyanko
1, 3, 4, 5, 6
,
Dmitry A Ruchkin
1, 3, 4, 5, 6
,
Sergey Bukhdruker
2, 8, 9, 10
,
Alina Remeeva
2
,
Alexey Mishin
2, 6, 7, 11
,
Valentin Borshchevskiy
2, 8, 9
,
Valentin Gordeliy
2, 8, 9, 12
,
Alexander S. Arseniev
1, 3, 4, 5, 6
,
Dmitriy A Gorbachev
1, 3, 4, 5, 6
,
Alexey S Gavrikov
1, 3, 4, 5, 6
,
Alexander S. Mishin
1, 3, 4, 5, 6
,
Mikhail Baranov
1, 3, 4, 5, 6, 13, 14
3
Institute of Bioorganic Chemistry
4
RUSSIAN ACADEMY OF SCIENCES
5
117997 Moscow
6
Russia
|
11
Dolgoprudny
|
12
Publication type: Journal Article
Publication date: 2021-04-08
scimago Q1
wos Q1
SJR: 2.138
CiteScore: 12.6
Impact factor: 7.4
ISSN: 20416520, 20416539
PubMed ID:
34040747
General Chemistry
Abstract
One of the essential characteristics of any tag used in bioscience and medical applications is its size. The larger the label, the more it may affect the studied object, and the more it may distort its behavior. In this paper, using NMR spectroscopy and X-ray crystallography, we have studied the structure of fluorogen-activating protein FAST both in the apo form and in complex with the fluorogen. We showed that significant change in the protein occurs upon interaction with the ligand. While the protein is completely ordered in the complex, its apo form is characterized by higher mobility and disordering of its N-terminus. We used structural information to design the shortened FAST (which we named nanoFAST) by truncating 26 N-terminal residues. Thus, we created the shortest genetically encoded tag among all known fluorescent and fluorogen-activating proteins, which is composed of only 98 amino acids.
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36
Total citations:
36
Citations from 2024:
16
(44%)
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MLA
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GOST
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Mineev K. S. et al. NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids // Chemical Science. 2021. Vol. 12. No. 19. pp. 6719-6725.
GOST all authors (up to 50)
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Mineev K. S. et al. NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids // Chemical Science. 2021. Vol. 12. No. 19. pp. 6719-6725.
Cite this
RIS
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TY - JOUR
DO - 10.1039/d1sc01454d
UR - https://xlink.rsc.org/?DOI=D1SC01454D
TI - NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids
T2 - Chemical Science
AU - Mineev, Konstantin S.
AU - Goncharuk, Sergey A
AU - Goncharuk, Marina V
AU - Povarova, Natalia V
AU - Sokolov, Anatolii I
AU - Baleeva, Nadezhda S
AU - Smirnov, Alexander Yu
AU - Myasnyanko, I. N.
AU - Ruchkin, Dmitry A
AU - Bukhdruker, Sergey
AU - Remeeva, Alina
AU - Mishin, Alexey
AU - Borshchevskiy, Valentin
AU - Gordeliy, Valentin
AU - Arseniev, Alexander S.
AU - Gorbachev, Dmitriy A
AU - Gavrikov, Alexey S
AU - Mishin, Alexander S.
AU - Baranov, Mikhail
PY - 2021
DA - 2021/04/08
PB - Royal Society of Chemistry (RSC)
SP - 6719-6725
IS - 19
VL - 12
PMID - 34040747
SN - 2041-6520
SN - 2041-6539
ER -
Cite this
BibTex (up to 50 authors)
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@article{2021_Mineev,
author = {Konstantin S. Mineev and Sergey A Goncharuk and Marina V Goncharuk and Natalia V Povarova and Anatolii I Sokolov and Nadezhda S Baleeva and Alexander Yu Smirnov and I. N. Myasnyanko and Dmitry A Ruchkin and Sergey Bukhdruker and Alina Remeeva and Alexey Mishin and Valentin Borshchevskiy and Valentin Gordeliy and Alexander S. Arseniev and Dmitriy A Gorbachev and Alexey S Gavrikov and Alexander S. Mishin and Mikhail Baranov and others},
title = {NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids},
journal = {Chemical Science},
year = {2021},
volume = {12},
publisher = {Royal Society of Chemistry (RSC)},
month = {apr},
url = {https://xlink.rsc.org/?DOI=D1SC01454D},
number = {19},
pages = {6719--6725},
doi = {10.1039/d1sc01454d}
}
Cite this
MLA
Copy
Mineev, Konstantin S., et al. “NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids.” Chemical Science, vol. 12, no. 19, Apr. 2021, pp. 6719-6725. https://xlink.rsc.org/?DOI=D1SC01454D.