Open Access
NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids
Тип публикации: Journal Article
Дата публикации: 2021-04-08
SCImago Q1
Tоп 10% SCImago
WOS Q1
БС1
SJR: 2.011
CiteScore: 12
Impact factor: 8.1
ISSN: 20416520, 20416539
PubMed ID:
34040747
General Chemistry
Краткое описание
One of the essential characteristics of any tag used in bioscience and medical applications is its size. The larger the label, the more it may affect the studied object, and the more it may distort its behavior. In this paper, using NMR spectroscopy and X-ray crystallography, we have studied the structure of fluorogen-activating protein FAST both in the apo form and in complex with the fluorogen. We showed that significant change in the protein occurs upon interaction with the ligand. While the protein is completely ordered in the complex, its apo form is characterized by higher mobility and disordering of its N-terminus. We used structural information to design the shortened FAST (which we named nanoFAST) by truncating 26 N-terminal residues. Thus, we created the shortest genetically encoded tag among all known fluorescent and fluorogen-activating proteins, which is composed of only 98 amino acids.
Найдено
Ничего не найдено, попробуйте изменить настройки фильтра.
Для доступа к списку цитирований публикации необходимо авторизоваться.
Для доступа к списку профилей, цитирующих публикацию, необходимо авторизоваться.
Топ-30
Журналы
|
1
2
3
4
5
6
7
|
|
|
Russian Journal of Bioorganic Chemistry
7 публикаций, 17.5%
|
|
|
bioRxiv
6 публикаций, 15%
|
|
|
International Journal of Molecular Sciences
5 публикаций, 12.5%
|
|
|
Nature Communications
3 публикации, 7.5%
|
|
|
Communications Biology
2 публикации, 5%
|
|
|
Chemical Science
2 публикации, 5%
|
|
|
Physical Chemistry Chemical Physics
2 публикации, 5%
|
|
|
Molecular Catalysis
2 публикации, 5%
|
|
|
Biomolecules
1 публикация, 2.5%
|
|
|
Microorganisms
1 публикация, 2.5%
|
|
|
Accounts of Chemical Research
1 публикация, 2.5%
|
|
|
Chemical Communications
1 публикация, 2.5%
|
|
|
Chemical Reviews
1 публикация, 2.5%
|
|
|
Journal of Cell Biology
1 публикация, 2.5%
|
|
|
Current Opinion in Chemical Biology
1 публикация, 2.5%
|
|
|
Proceedings of the National Academy of Sciences of the United States of America
1 публикация, 2.5%
|
|
|
Arabian Journal of Chemistry
1 публикация, 2.5%
|
|
|
ACS Synthetic Biology
1 публикация, 2.5%
|
|
|
Nature Chemical Biology
1 публикация, 2.5%
|
|
|
1
2
3
4
5
6
7
|
Издатели
|
1
2
3
4
5
6
7
|
|
|
MDPI
7 публикаций, 17.5%
|
|
|
Pleiades Publishing
7 публикаций, 17.5%
|
|
|
Springer Nature
6 публикаций, 15%
|
|
|
openRxiv
6 публикаций, 15%
|
|
|
Royal Society of Chemistry (RSC)
5 публикаций, 12.5%
|
|
|
American Chemical Society (ACS)
3 публикации, 7.5%
|
|
|
Elsevier
3 публикации, 7.5%
|
|
|
Rockefeller University Press
1 публикация, 2.5%
|
|
|
Proceedings of the National Academy of Sciences (PNAS)
1 публикация, 2.5%
|
|
|
Scientific Scholar
1 публикация, 2.5%
|
|
|
1
2
3
4
5
6
7
|
- Мы не учитываем публикации, у которых нет DOI.
- Статистика публикаций обновляется еженедельно.
Вы ученый?
Создайте профиль, чтобы получать персональные рекомендации коллег, конференций и новых статей.
Войти с ORCID
Метрики
40
Всего цитирований:
40
Цитирований c 2025:
12
(30%)
Цитировать
ГОСТ |
RIS |
BibTex |
MLA
Цитировать
ГОСТ
Скопировать
Mineev K. S. et al. NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids // Chemical Science. 2021. Vol. 12. No. 19. pp. 6719-6725.
ГОСТ со всеми авторами (до 50)
Скопировать
Mineev K. S. et al. NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids // Chemical Science. 2021. Vol. 12. No. 19. pp. 6719-6725.
Цитировать
RIS
Скопировать
TY - JOUR
DO - 10.1039/d1sc01454d
UR - https://xlink.rsc.org/?DOI=D1SC01454D
TI - NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids
T2 - Chemical Science
AU - Mineev, Konstantin S.
AU - Goncharuk, Sergey A
AU - Goncharuk, Marina V
AU - Povarova, Natalia V
AU - Sokolov, Anatolii I
AU - Baleeva, Nadezhda S
AU - Smirnov, Alexander Yu
AU - Myasnyanko, I. N.
AU - Ruchkin, Dmitry A
AU - Bukhdruker, Sergey
AU - Remeeva, Alina
AU - Mishin, Alexey
AU - Borshchevskiy, Valentin
AU - Gordeliy, Valentin
AU - Arseniev, Alexander S.
AU - Gorbachev, Dmitriy A
AU - Gavrikov, Alexey S
AU - Mishin, Alexander S.
AU - Baranov, Mikhail
PY - 2021
DA - 2021/04/08
PB - Royal Society of Chemistry (RSC)
SP - 6719-6725
IS - 19
VL - 12
PMID - 34040747
SN - 2041-6520
SN - 2041-6539
ER -
Цитировать
BibTex (до 50 авторов)
Скопировать
@article{2021_Mineev,
author = {Konstantin S. Mineev and Sergey A Goncharuk and Marina V Goncharuk and Natalia V Povarova and Anatolii I Sokolov and Nadezhda S Baleeva and Alexander Yu Smirnov and I. N. Myasnyanko and Dmitry A Ruchkin and Sergey Bukhdruker and Alina Remeeva and Alexey Mishin and Valentin Borshchevskiy and Valentin Gordeliy and Alexander S. Arseniev and Dmitriy A Gorbachev and Alexey S Gavrikov and Alexander S. Mishin and Mikhail Baranov and others},
title = {NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids},
journal = {Chemical Science},
year = {2021},
volume = {12},
publisher = {Royal Society of Chemistry (RSC)},
month = {apr},
url = {https://xlink.rsc.org/?DOI=D1SC01454D},
number = {19},
pages = {6719--6725},
doi = {10.1039/d1sc01454d}
}
Цитировать
MLA
Скопировать
Mineev, Konstantin S., et al. “NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids.” Chemical Science, vol. 12, no. 19, Apr. 2021, pp. 6719-6725. https://xlink.rsc.org/?DOI=D1SC01454D.
Лаборатории
Ошибка в публикации?