The use of nanosized liposomes from vegetable phospholipids in combination with albumine and some polysaccharides as cryoprotective agents in the course of cryopreservation

It is known that nanosized liposomes from vegetable and animal phospholipids promote survival and functional adequacy of animal cells in the course of cryopreservation. It is also known that albumine and some polysaccharides have membrane-protective properties. In this paper we investigated whether there is a synergism of cryoprotective action between soybean liposomes and macromolecular compounds: albumine, sodium alginate, methylcellulose, carageenan and hyaluronic acid. To determine the cryoprotective effect we carried out cryopreservation of bull semen using a standard procedure in the presence of the substances under investigation. Albumine and sodium alginate demonstrated the most considerable cryoprotective effect in the presence of liposomes. In addition we determined dimensional characteristics of nanoparticles in a liposome suspension without and with sodium alginate and studied the influence of autoclave treatment on the nanoparticle size and cryoprotective effect of the complex. Autoclaving caused enlargement of a small-sized fraction of nanoparticles, supposedly, sodium alginate aggregates. Besides, the autoclaving did not demonstrate any influence on the cryoprotective efficiency of the mixture.