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Nucleic Acids Research, volume 49, issue 18, pages 10524-10541

Suppression of liquid-liquid phase separation by 1,6-hexanediol partially compromises the 3D genome organization in living cells

Velichko Artem K. 2, 3, 4
Magnitov Mikhail D 2, 4, 5
Luzhin Artem V. 2, 4
Golov Arkadiy K 2
Kireev I. 6, 7
Tyakht Alexander 2, 4
Gavrilov Alexey 2, 4
Publication typeJournal Article
Publication date2021-04-09
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor14.9
ISSN03051048, 13624962
PubMed ID:  33836078
Genetics
Abstract

Liquid–liquid phase separation (LLPS) contributes to the spatial and functional segregation of molecular processes within the cell nucleus. However, the role played by LLPS in chromatin folding in living cells remains unclear. Here, using stochastic optical reconstruction microscopy (STORM) and Hi-C techniques, we studied the effects of 1,6-hexanediol (1,6-HD)-mediated LLPS disruption/modulation on higher-order chromatin organization in living cells. We found that 1,6-HD treatment caused the enlargement of nucleosome clutches and their more uniform distribution in the nuclear space. At a megabase-scale, chromatin underwent moderate but irreversible perturbations that resulted in the partial mixing of A and B compartments. The removal of 1,6-HD from the culture medium did not allow chromatin to acquire initial configurations, and resulted in more compact repressed chromatin than in untreated cells. 1,6-HD treatment also weakened enhancer-promoter interactions and TAD insulation but did not considerably affect CTCF-dependent loops. Our results suggest that 1,6-HD-sensitive LLPS plays a limited role in chromatin spatial organization by constraining its folding patterns and facilitating compartmentalization at different levels.

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Ulianov S. V. et al. Suppression of liquid-liquid phase separation by 1,6-hexanediol partially compromises the 3D genome organization in living cells // Nucleic Acids Research. 2021. Vol. 49. No. 18. pp. 10524-10541.
GOST all authors (up to 50) Copy
Ulianov S. V., Velichko A. K., Magnitov M. D., Luzhin A. V., Golov A. K., Ovsyannikova N., Kireev I., Gavrikov A. S., Mishin A. S., Garaev A. K., Tyakht A., Gavrilov A., Kantidze O. L., Разин С. В. Suppression of liquid-liquid phase separation by 1,6-hexanediol partially compromises the 3D genome organization in living cells // Nucleic Acids Research. 2021. Vol. 49. No. 18. pp. 10524-10541.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1093/NAR/GKAB249
UR - https://doi.org/10.1093%2FNAR%2FGKAB249
TI - Suppression of liquid-liquid phase separation by 1,6-hexanediol partially compromises the 3D genome organization in living cells
T2 - Nucleic Acids Research
AU - Ulianov, Sergey V.
AU - Magnitov, Mikhail D
AU - Garaev, Azat K
AU - Tyakht, Alexander
AU - Kantidze, Omar L.
AU - Разин, С. В.
AU - Velichko, Artem K.
AU - Golov, Arkadiy K
AU - Gavrikov, Alexey S
AU - Luzhin, Artem V.
AU - Ovsyannikova, Natalia
AU - Kireev, I.
AU - Mishin, Alexander S.
AU - Gavrilov, Alexey
PY - 2021
DA - 2021/04/09 00:00:00
PB - Oxford University Press
SP - 10524-10541
IS - 18
VL - 49
PMID - 33836078
SN - 0305-1048
SN - 1362-4962
ER -
BibTex |
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BibTex Copy
@article{2021_Ulianov
author = {Sergey V. Ulianov and Mikhail D Magnitov and Azat K Garaev and Alexander Tyakht and Omar L. Kantidze and С. В. Разин and Artem K. Velichko and Arkadiy K Golov and Alexey S Gavrikov and Artem V. Luzhin and Natalia Ovsyannikova and I. Kireev and Alexander S. Mishin and Alexey Gavrilov},
title = {Suppression of liquid-liquid phase separation by 1,6-hexanediol partially compromises the 3D genome organization in living cells},
journal = {Nucleic Acids Research},
year = {2021},
volume = {49},
publisher = {Oxford University Press},
month = {apr},
url = {https://doi.org/10.1093%2FNAR%2FGKAB249},
number = {18},
pages = {10524--10541},
doi = {10.1093/NAR/GKAB249}
}
MLA
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MLA Copy
Ulianov, Sergey V., et al. “Suppression of liquid-liquid phase separation by 1,6-hexanediol partially compromises the 3D genome organization in living cells.” Nucleic Acids Research, vol. 49, no. 18, Apr. 2021, pp. 10524-10541. https://doi.org/10.1093%2FNAR%2FGKAB249.
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