Open Access
Nucleic Acids Research, volume 33, issue 18, pages 6048-6056
Function of the ribosomal E-site: A mutagenesis study
Publication type: Journal Article
Publication date: 2005-10-12
Journal:
Nucleic Acids Research
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 14.9
ISSN: 03051048, 13624962
PubMed ID:
16243787
Genetics
Abstract
Ribosomes synthesize proteins according to the information encoded in mRNA. During this process, both the incoming amino acid and the nascent peptide are bound to tRNA molecules. Three binding sites for tRNA in the ribosome are known: the A-site for aminoacyl-tRNA, the P-site for peptidyl-tRNA and the E-site for the deacylated tRNA leaving the ribosome. Here, we present a study of Escherichia coli ribosomes with the E-site binding destabilized by mutation C2394G of the 23S rRNA. Expression of the mutant 23S rRNA in vivo caused increased frameshifting and stop codon readthrough. The progression of these ribosomes through the ribosomal elongation cycle in vitro reveals ejection of deacylated tRNA during the translocation step or shortly after. E-site compromised ribosomes can undergo translocation, although in some cases it is less efficient and results in a frameshift. The mutation affects formation of the P/E hybrid site and leads to a loss of stimulation of the multiple turnover GTPase activity of EF-G by deacylated tRNA bound to the ribosome.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Sergiev P. V. Function of the ribosomal E-site: A mutagenesis study // Nucleic Acids Research. 2005. Vol. 33. No. 18. pp. 6048-6056.
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Sergiev P. V. Function of the ribosomal E-site: A mutagenesis study // Nucleic Acids Research. 2005. Vol. 33. No. 18. pp. 6048-6056.
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RIS
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TY - JOUR
DO - 10.1093/nar/gki910
UR - https://doi.org/10.1093%2Fnar%2Fgki910
TI - Function of the ribosomal E-site: A mutagenesis study
T2 - Nucleic Acids Research
AU - Sergiev, P V
PY - 2005
DA - 2005/10/12 00:00:00
PB - Oxford University Press
SP - 6048-6056
IS - 18
VL - 33
PMID - 16243787
SN - 0305-1048
SN - 1362-4962
ER -
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@article{2005_Sergiev,
author = {P V Sergiev},
title = {Function of the ribosomal E-site: A mutagenesis study},
journal = {Nucleic Acids Research},
year = {2005},
volume = {33},
publisher = {Oxford University Press},
month = {oct},
url = {https://doi.org/10.1093%2Fnar%2Fgki910},
number = {18},
pages = {6048--6056},
doi = {10.1093/nar/gki910}
}
Cite this
MLA
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Sergiev, P. V.. “Function of the ribosomal E-site: A mutagenesis study.” Nucleic Acids Research, vol. 33, no. 18, Oct. 2005, pp. 6048-6056. https://doi.org/10.1093%2Fnar%2Fgki910.
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