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Structure and function of the N-terminal domain of the yeast telomerase reverse transcriptase

Petrova O.A., Mantsyzov A.B., Rodina E.V., Efimov S.V., Hackenberg C., Hakanpää J., Klochkov V.V., Lebedev A.A., Chugunova A.A., Malyavko A.N., Zatsepin T.S., Mishin A.V., Zvereva M.I., Lamzin V.S., Dontsova O.A., Polshakov V.I.
Тип документаJournal Article
Дата публикации2018-02-16
Название журналаNucleic Acids Research
ИздательOxford University Press
Квартиль по SCImagoQ1
Квартиль по Web of ScienceQ1
Импакт-фактор 202119.16
ISSN03051048, 13624962
Genetics
Краткое описание
Abstract The elongation of single-stranded DNA repeats at the 3′-ends of chromosomes by telomerase is a key process in maintaining genome integrity in eukaryotes. Abnormal activation of telomerase leads to uncontrolled cell division, whereas its down-regulation is attributed to ageing and several pathologies related to early cell death. Telomerase function is based on the dynamic interactions of its catalytic subunit (TERT) with nucleic acids—telomerase RNA, telomeric DNA and the DNA/RNA heteroduplex. Here, we present the crystallographic and NMR structures of the N-terminal (TEN) domain of TERT from the thermotolerant yeast Hansenula polymorpha and demonstrate the structural conservation of the core motif in evolutionarily divergent organisms. We identify the TEN residues that are involved in interactions with the telomerase RNA and in the recognition of the ‘fork’ at the distal end of the DNA product/RNA template heteroduplex. We propose that the TEN domain assists telomerase biological function and is involved in restricting the size of the heteroduplex during telomere repeat synthesis.
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ГОСТ |
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1. Petrova O.A. и др. Structure and function of the N-terminal domain of the yeast telomerase reverse transcriptase // Nucleic Acids Research. 2017. Т. 46. № 3. С. 1525–1540.
RIS |
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TY - JOUR

DO - 10.1093/nar/gkx1275

UR - http://dx.doi.org/10.1093/nar/gkx1275

TI - Structure and function of the N-terminal domain of the yeast telomerase reverse transcriptase

T2 - Nucleic Acids Research

AU - Petrova, Olga A

AU - Mantsyzov, Alexey B

AU - Rodina, Elena V

AU - Efimov, Sergey V

AU - Hackenberg, Claudia

AU - Hakanpää, Johanna

AU - Klochkov, Vladimir V

AU - Lebedev, Andrej A

AU - Chugunova, Anastasia A

AU - Malyavko, Alexander N

AU - Zatsepin, Timofei S

AU - Mishin, Alexey V

AU - Zvereva, Maria I

AU - Lamzin, Victor S

AU - Dontsova, Olga A

AU - Polshakov, Vladimir I

PY - 2017

DA - 2017/12/23

PB - Oxford University Press (OUP)

SP - 1525-1540

IS - 3

VL - 46

SN - 0305-1048

SN - 1362-4962

ER -

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@article{2017,

doi = {10.1093/nar/gkx1275},

url = {https://doi.org/10.1093%2Fnar%2Fgkx1275},

year = 2017,

month = {dec},

publisher = {Oxford University Press ({OUP})},

volume = {46},

number = {3},

pages = {1525--1540},

author = {Olga A Petrova and Alexey B Mantsyzov and Elena V Rodina and Sergey V Efimov and Claudia Hackenberg and Johanna Hakanpää and Vladimir V Klochkov and Andrej A Lebedev and Anastasia A Chugunova and Alexander N Malyavko and Timofei S Zatsepin and Alexey V Mishin and Maria I Zvereva and Victor S Lamzin and Olga A Dontsova and Vladimir I Polshakov},

title = {Structure and function of the N-terminal domain of the yeast telomerase reverse transcriptase}

}

MLA
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Petrova, Olga A et al. “Structure and Function of the N-Terminal Domain of the Yeast Telomerase Reverse Transcriptase.” Nucleic Acids Research 46.3 (2017): 1525–1540. Crossref. Web.