Developmental dynamics of autonomic function in childhood

Johnson M., Deardorff J., Davis E.L., Martinez W., Eskenazi B., Alkon A.

Adversity, such as living in poor socioeconomic conditions during early childhood, can become embedded in children's physiology and deleteriously affect their health later in life. On the other hand, maternal responsivity may have adaptive effects on physiology during early childhood development. The current study tested both the additive and interactive effects of socioeconomic status (SES) and maternal responsivity measured at 1year of age on resting autonomic nervous system (ANS) function and trajectory during the first 5years of life. Participants came from a birth cohort comprised of Mexican-origin families living in California. Children's resting ANS functioning (respiratory sinus arrhythmia; RSA; pre-ejection period; PEP; and heart rate; HR) was collected at 1, 3.5, and 5years of age (N=336) and modeled across time using Hierarchical Linear Modeling. Consistent with hypotheses, results showed that low SES predicted flatter trajectories of resting HR and PEP over early childhood (i.e., patterns of consistently higher heart rate; shorter PEP), whereas children who experienced positive maternal responsivity had steeper trajectories in RSA and PEP over time (i.e., increasing parasympathetic activation; decreasing sympathetic activation). The interaction between SES and maternal responsivity significantly predicted RSA intercept at age 5, such that among children living in low SES environments, high maternal responsivity mitigated the negative effect of poverty and predicted higher resting RSA at 5years of age. Results are consistent with the early life programming theory that suggests that environmental influences become biologically embedded in the physiology of children living in socially disadvantaged contexts, and identify increased maternal responsivity as a developmental mechanism that could offset the deleterious effects of low SES.

Giuliano R.J., Gatzke-Kopp L.M., Roos L.E., Skowron E.A.

The neurovisceral integration model stipulates that autonomic function plays a critical role in the regulation of higher-order cognitive processes, yet most work to date has examined parasympathetic function in isolation from sympathetic function. Furthermore, the majority of work has been conducted on normative samples, which typically demonstrate parasympathetic withdrawal to increase arousal needed to complete cognitive tasks. Little is known about how autonomic regulation supports cognitive function in populations exposed to high levels of stress, which is critical given that chronic stress exposure alters autonomic function. To address this, we sought to characterize how parasympathetic (high-frequency heart rate variability, HF-HRV) and sympathetic (preejection period, PEP) measures of cardiac function contribute to individual differences in working memory (WM) capacity in a sample of high-risk women. HF-HRV and PEP were measured at rest and during a visual change detection measure of WM. Multilevel modeling was used to examine within-person fluctuations in WM performance throughout the task concurrently with HF-HRV and PEP, as well as between-person differences as a function of resting HF-HRV and PEP levels. Results indicate that resting PEP moderated the association between HF-HRV reactivity and WM capacity. Increases in WM capacity across the task were associated with increases in parasympathetic activity, but only among individuals with longer resting PEP (lower sympathetic arousal). Follow-up analyses showed that shorter resting PEP was associated with greater cumulative risk exposure. These results support the autonomic space framework, in that the relationship between behavior and parasympathetic function appears dependent on resting sympathetic activation.

Iacono W.G., Malone S.M., Vrieze S.I.

This review examines the current state of electrophysiological endophenotype research and recommends best practices that are based on knowledge gleaned from the last decade of molecular genetic research with complex traits. Endophenotype research is being oversold for its potential to help discover psychopathology relevant genes using the types of small samples feasible for electrophysiological research. This is largely because the genetic architecture of endophenotypes appears to be very much like that of behavioral traits and disorders: they are complex, influenced by many variants (e.g., tens of thousands) within many genes, each contributing a very small effect. Out of over 40 electrophysiological endophenotypes covered by our review, only resting heart, a measure that has received scant advocacy as an endophenotype, emerges as an electrophysiological variable with verified associations with molecular genetic variants. To move the field forward, investigations designed to discover novel variants associated with endophenotypes will need extremely large samples best obtained by forming consortia and sharing data obtained from genome wide arrays. In addition, endophenotype research can benefit from successful molecular genetic studies of psychopathology by examining the degree to which these verified psychopathology-relevant variants are also associated with an endophenotype, and by using knowledge about the functional significance of these variants to generate new endophenotypes. Even without molecular genetic associations, endophenotypes still have value in studying the development of disorders in unaffected individuals at high genetic risk, constructing animal models, and gaining insight into neural mechanisms that are relevant to clinical disorder.

Brugnera A., Adorni R., Compare A., Zarbo C., Sakatani K.

Abstract. Emotions characterized by opposite valences (positive vs. negative) seem to lead to specific patterns of autonomic and cortical activity. For example, according to valence or approach-withdrawal hypotheses, specific emotions lead to an asymmetrical activation of left or right prefrontal cortex (PFC). The aim of the present study was to explore the psychophysiological underpinnings of emotion experiencing using a paradigm with higher ecological validity than is typically accomplished in neuroimaging research. A total of 28 healthy participants were instructed to recall personally-relevant situations from the past that caused positive (happiness) or negative (anger) emotions, during a 2 min silent preparatory phase and a subsequent 3 min verbal phase. A wearable electrocardiographic (ECG) recording system and a portable 2-channel near-infrared spectroscopy (NIRS) device were used to collect heart rate (HR), high frequencies of heart rate variability (HF-HRV), and the hemodynamic responses of prefrontal cortex during the entire procedure. Results showed that during both anger and happiness recall tasks, HR increased and high frequencies of HRV decreased with respect to the baseline. HR and HF-HRV reached, respectively, their highest and lowest points during the verbal phase of anger recall task. NIRS data evidenced a bilateral increase of oxyhemoglobin concentration changes during both anger and happiness recall tasks, which was highest during the verbal phases. However, no lateralization patterns were found. Overall, present results suggest that the experience of negative emotions, if compared with positive ones, is characterized by a combination of reduced parasympathetic activation and/or increased sympathetic activation. Thus, cardiological data provided partial support to autonomic specificity of emotions. However, the recall paradigm did not evidence an asymmetry of PFC activity during the experience of emotions with opposite valences, probably due to the high number of factors impacting prefrontal activity during a recall paradigm.

Wright R.J., Rakow T.

Miller G.A., Rockstroh B.S., Hamilton H.K., Yee C.M.

The Research Domain Criteria (RDoC) initiative of the National Institute of Mental Health shows great promise in providing guidance for research on mental illness but has prompted considerable controversy. Papers by Yancey, Venables, and Patrick and Kozak and Cuthbert illustrate and clarify a number of important features of RDoC. The present commentary evaluates the former paper in light of the latter paper and addresses several common misunderstandings about RDoC. The concept of endophenotypes and diverse psychophysiological approaches will likely be central in RDoC-inspired research.

Waters S.F., Boyce W.T., Eskenazi B., Alkon A.

The study of autonomic nervous system responses and contextual factors has shed light on the development of children's negative outcomes, but the majority of these studies have not focused on minority populations living under adversity. To address these gaps, the current longitudinal study included a sample of poor, immigrant Latino families to examine whether associations between children's autonomic nervous system reactivity at 6 months and their externalizing behavior problems at 7 years of age were moderated by two risk factors associated with poverty: the interpersonal factor of chronic maternal depression and the environmental factor of chronic overcrowded housing. Multiple linear regression (N = 99) revealed that children who exhibited less parasympathetic nervous system withdrawal in response to challenge during infancy had more externalizing problems during childhood only if they had mothers who experienced chronic depression. Children who exhibited greater sympathetic nervous system reactivity during infancy had the lowest levels of externalizing problems during childhood only if they had mothers who chronic depression. Chronic overcrowded housing did not moderate associations between physiological reactivity and level of externalizing problems. These findings extend our understanding of the interaction of physiology and context on child outcomes to the understudied population of impoverished Latino families.

Gatzke-Kopp L.M.

This Special Issue is devoted to the illustration and discussion of three key demographic variables (sex/gender, race/ethnicity, and socioeconomic status) that have been shown to moderate associations between psychophysiological processes and behavior. The introduction to the issue discusses the role of phenotypic plasticity in the emergence of different neural processes that achieve the same behavioral outcome, with emphasis on how these relatively stable developmental contexts affect brain/behavior associations without necessarily resulting in difference in behavior. These findings have profound significance for the implications of generalization and call into question the presumption that diverse samples produce an average result that is appropriately reflective of the individuals themselves. Increasing diversity within psychophysiological research is critical in elucidating mechanisms by which the human brain can accomplish cognitive and affective behaviors. This article further examines the logistical and ethical challenges faced in achieving this goal.

Kuzawa C.W., Chugani H.T., Grossman L.I., Lipovich L., Muzik O., Hof P.R., Wildman D.E., Sherwood C.C., Leonard W.R., Lange N.

Significance The metabolic costs of brain development are thought to explain the evolution of humans’ exceptionally slow and protracted childhood growth; however, the costs of the human brain during development are unknown. We used existing PET and MRI data to calculate brain glucose use from birth to adulthood. We find that the brain’s metabolic requirements peak in childhood, when it uses glucose at a rate equivalent to 66% of the body’s resting metabolism and 43% of the body’s daily energy requirement, and that brain glucose demand relates inversely to body growth from infancy to puberty. Our findings support the hypothesis that the unusually high costs of human brain development require a compensatory slowing of childhood body growth.

Mills-Koonce W.R., Wagner N.J., Willoughby M.T., Stifter C., Blair C., Granger D.A.

Gatzke-Kopp L.M., Greenberg M., Bierman K.

Following theories that individual differences in respiratory sinus arrhythmia (RSA) denote differential sensitivity to environmental influences, this study examines whether differences in RSA reactivity to specific emotional challenges predict differential response to intervention. We present data from a randomized clinical trial of a targeted intervention for early onset aggression. In collaboration with a high-risk urban school district, 207 kindergarten children (73% African American, 66% male), identified by their teachers as having high levels of aggressive and disruptive behavior, were recruited. All children received a universal social-emotional curriculum. One hundred children were randomly assigned to an additional intervention consisting of weekly peer-based social skills training. Complete RSA data were available for 139 of the children. Teacher-reported externalizing symptoms and emotion regulation in 1st grade (post intervention) were examined controlling for baseline levels. First-grade peer nominations of aggressive behavior, controlling for baseline nominations, were also examined as outcomes. No effect of resting RSA was found. However, greater reactivity to anger was associated with higher externalizing symptoms and lower emotion regulation skills in 1st grade relative to low reactive children. Lower reactivity to fear was associated with greater improvement over time, an effect that was enhanced in the targeted intervention condition. Results suggest that measures of affective reactivity may provide insight into children's capacity to benefit from different types of interventions.

Baker E., Shelton K.H., Baibazarova E., Hay D.F., van Goozen S.H.

Low autonomic nervous system activity is claimed to be a biomarker for aggressive and antisocial behavior. Although there is evidence that low skin conductance activity (SCA) accounts for variation in the severity of antisocial behavior and predicts the onset of aggression in children and adults, it is unknown whether SCA measured in infancy can predict the development of aggression. We measured SCA in 70 typically developing 1-year-old infants at baseline, during an orienting habituation paradigm, and during a fear challenge. We also observed the infants’ fear behavior, and each mother rated her infant’s temperament and her attachment to her child. At follow-up, mothers rated the children at 3 years old for aggressive and nonaggressive behavior problems. Low infant SCA predicted aggressive behavior, but there was no association between SCA and nonaggressive behavior problems. Mothers’ ratings of the infants’ temperament and their maternal attachment and the infants’ observed fearlessness did not predict later aggression. These results suggest that SCA is a specific biomarker for aggression in low-risk samples of infants.

Hinnant J.B., El-Sheikh M., Keiley M., Buckhalt J.A.

Relations between marital conflict, children's respiratory sinus arrhythmia (RSA), and fluid cognitive performance were examined over 3 years to assess allostatic processes. Participants were 251 children reporting on marital conflict, baseline RSA, and RSA reactivity (RSA-R) to a lab challenge were recorded, and fluid cognitive performance was measured using the Woodcock–Johnson III. A cross-lagged model showed that higher levels of marital conflict at age 8 predicted weaker RSA-R at age 9 for children with lower baseline RSA. A growth model showed that lower baseline RSA in conjunction with weaker RSA-R predicted the slowest development of fluid cognitive performance. Findings suggest that stress may affect development of physiological systems regulating attention, which are tied to the development of fluid cognitive performance.

Fortunato C.K., Gatzke-Kopp L.M., Ram N.

Internalizing and externalizing disorders are often, though inconsistently in studies of young children, associated with low baseline levels of respiratory sinus arrhythmia (RSA). RSA is thus considered to reflect the capacity for flexible and regulated affective reactivity and a general propensity for psychopathology. However, studies assessing RSA reactivity to emotional challenges tend to report more consistent associations with internalizing than with externalizing disorders, although it is unclear whether this is a function of the type of emotion challenges used. In the present study, we examined whether baseline RSA was associated with internalizing and/or externalizing severity in a sample of 273 young children (ages 5–6) with elevated symptoms of psychopathology. Following motivation-based models of emotion, we also tested whether RSA reactivity during withdrawal-based (fear, sadness) and approach-based (happiness, anger) emotion inductions was differentially associated with internalizing and externalizing symptoms, respectively. Baseline RSA was not associated with externalizing or internalizing symptom severity. However, RSA reactivity to specific emotional challenges was associated differentially with each symptom domain. As expected, internalizing symptom severity was associated with greater RSA withdrawal (increased arousal) during fearful and sad film segments. Conversely, externalizing symptom severity was related to blunted RSA withdrawal during a happy film segment. The use of theoretically derived stimuli may be important in characterizing the nature of the deficits in emotion processing that differentiate the internalizing and externalizing domains of psychopathology.

Gatzke-Kopp L.M., Jetha M.K., Segalowitz S.J.

Resting EEG asymmetry evident early in life is thought to bias affective behaviors and contribute to the development of psychopathology. However, it remains unclear at what stage of information processing this bias occurs. Asymmetry may serve as an afferent filter, modulating emotional reactivity to incoming stimuli; or as an efferent filter, modulating behavioral response tendencies under emotional conditions. This study examines 209 kindergarten children (M = 6.03 years old) to test predictions put forth by the two models. Resting asymmetry was examined in conjunction with electrodermal and cardiac measures of physiological reactivity to four emotion-inducing film clips (fear, sad, happy, anger) and teacher ratings of psychopathology. Results confirm an association between increased right side cortical activation and internalizing symptom severity as well as left activation and externalizing symptom severity. Significant interactions between resting asymmetry and physiological reactivity to emotion indicate that physiological reactivity moderates the association between resting asymmetry and symptoms of psychopathology.

Shvartz V., Danilov V., Koroleva S., Shvartz E., Donakanyan S., Permyakov V., Erastova N., Golukhova E., Radikovna B.A., Viktorovich K.V., Andreevna F.A., Vitalievich M.V., Kazbekovna P.Z., Nikolaevich S.V., Andreevich Y.A., et. al.

To obtain reference ranges for heart rate variability (HRV) parameters in healthy children, taking into account their gender and age. The study included the results of testing 22,468 children ranging 5.5–12.5 years of age. The data were grouped by age and gender. The analysis of HRV parameters was carried out according to the standards described in existing guidelines. It was based on a 5-min rhythmogram obtained from a standard 12-lead electrocardiogram (ECG). The following characteristics of the temporal and spectral analyses of HRV were studied: standard deviation of NN intervals (SDNN), power in the low-frequency band of the HRV spectrum (LF), low-frequency power as mean percentage of total power (TP) of HRV spectrum (LF%), power in the high-frequency band of the HRV spectrum (HF), high-frequency power as mean percentage of TP of HRV spectrum (HF%), power in the very-low-frequency band of HRV (VLF), and LF/HF ratio. The values of SDNN, LF, HF, HF% and TP increased with age, reaching a peak at 8 and 9 years of age. It is notable that the values of SDNN, LF and TP were significantly lower in girls in each age group. The values of LF% and LF/HF decreased with age, with a peak decrease at 8 and 9 years of age, followed by a moderate increase or stabilization. The values of LF% and LF/HF did not differ statistically significantly between boys and girls. The VLF parameter exhibited a clear trend of growth with age and had higher values in boys of each age group. The correlation between HRV and heart rate (HR) was statistically significant. The correlation graphs had similar curves in both gender groups and both age groups. The parameters of the total HRV, as well as the activity of the parasympathetic nervous system (PNS), exhibited similar growth trends with age, with a peak increase in their values at 8 and 9 years of age. HR, as well as the activity of the sympathetic nervous system (SNS), decreased with age. Gender differences also followed a consistent pattern: parameters characterizing the activity of the peripheral nervous system were significantly higher in boys than in girls across all age groups.

Aldrete-Cortez V., Tafoya S.A.

Helánová K., Šišáková M., Hnatkova K., Novotný T., Andršová I., Malik M.

AbstractAutonomic control of heart rate is well known in adult subjects, but limited data are available on the development of the heart rate control during childhood and adolescence. Continuous 12-lead electrocardiograms were recorded in 1045 healthy children and adolescents (550 females) aged 4 to 19 years during postural manoeuvres involving repeated 10-min supine, unsupported sitting, and unsupported standing positions. In each position, heart rate was measured, and heart rate variability indices were evaluated (SDNN, RMSSD, and high (HF) and low (LF) frequency components were obtained). Quasi-normalized HF frequency components were defined as qnHF = HF/(HF + LF). These measurements were, among others, related to age using linear regressions. In supine position, heart rate decreases per year of age were significant in both sexes but lower in females than in males. In standing position, these decreases per year of age were substantially lowered. RMSSD and qnHF indices were independent of age in supine position but significantly decreased with age in sitting and standing positions. Correspondingly, LF/HF proportions showed steep increases with age in sitting and standing positions but not in the supine position. The study suggests that baseline supine parasympathetic influence shows little developmental changes during childhood and adolescence but that in young children, sympathetic branch is less responsive to vagal influence. While vagal influences modulate cardiac periods in young and older children equally, they are less able to suppress the sympathetic influence in younger children.

Lent M.C., Perry K.J., Perhamus G.R., Buck C., Murray-Close D., Ostrov J.M.

There are many benefits of peer interactions for children's social, emotional, and cognitive development, and isolation from peers may have negative consequences for children. Although biological processes may underlie social withdrawal broadly, distinct patterns may be associated with withdrawal behaviors depending on their underlying motivation (e.g., shy versus disinterested). This study investigated the role of autonomic nervous system activity, as assessed via skin conductance level (SCL) and respiratory sinus arrhythmia (RSA) in predicting changes in unsociability (e.g., lack of interest in peers) and anxious-fearfulness (e.g., discomfort among peers). Data were collected using a community sample of 92 US preschool children (45.7 % female; Mage = 45.51 months, SDage = 3.81 months) at two time points one year apart. Gender differences were also explored. Baseline physiology was assessed while viewing a neutral video clip, and reactivity was assessed while viewing social exclusion and post-aggression discussion videos. For all children, coinhibition (i.e., SCL inhibition accompanied by RSA inhibition) to the post-aggression discussion video and blunted SCL activation to the exclusion video were prospectively associated with higher levels of anxious-fearfulness one year later. For boys only, baseline reciprocal sympathetic activation (i.e., SCL activation and RSA inhibition) was prospectively related to higher levels of unsociability one year later. For girls only, RSA inhibition in response to the post-aggression discussion video was prospectively related to higher levels of unsociability one year later. Findings contribute to a growing literature on autonomic reactivity in preschoolers' adjustment and suggest possible differences in the physiological processes underlying unsociability and anxious-fearfulness.

Zhang X., Gatzke-Kopp L.M., Skowron E.A.

Abstract This study examined how temporal associations between parents’ physiological and behavioral responses may reflect underlying regulatory difficulties in at-risk parenting. Time-series data of cardiac indices (second-by-second estimates of inter-beat intervals – IBI, and respiratory sinus arrhythmia – RSA) and parenting behaviors were obtained from 204 child welfare-involved parents (88% mothers, Mage = 32.32 years) during child-led play with their 3- to 7-year-old children (45.1% female; Mage = 4.76 years). Known risk factors for maltreatment, including parents’ negative social cognitions, mental health symptoms, and inhibitory control problems, were examined as moderators of intra-individual physiology-behavior associations. Results of ordinary differential equations suggested increases in parents’ cardiac arousal at moments when they showed positive parenting behaviors. In turn, higher arousal was associated with momentary decreases in both positive and negative parenting behaviors. Individual differences in these dynamic processes were identified in association with parental risk factors. In contrast, no sample-wide RSA-behavior associations were evident, but a pattern of increased positive parenting at moments of parasympathetic withdrawal emerged among parents showing more total positive parenting behaviors. This study illustrated an innovative and ecologically-valid approach to examining regulatory patterns that may shape parenting in real-time and identified mechanisms that should be addressed in interventions.

Quigley K.M., Petty C.R., Sidamon‐Eristoff A.E., Modico M., Nelson C.A., Bosquet Enlow M.

AbstractIntergenerational transmission of internalizing disorders (anxiety and depression) is well documented, but the responsible pathways are underspecified. One possible mechanism is via programming of the child's parasympathetic nervous system (PNS). For example, maternal depression and anxiety, via multiple pathways, may heighten child PNS reactivity, which has been linked to increased risk for internalizing disorders. Heightened PNS reactivity also may sensitize a child to their environment, increasing the vulnerability to developing psychopathology when exposed to stressors, such as maternal psychopathology. In a prospective longitudinal study of mother–child dyads (N = 446), we examined relations among maternal depression and anxiety symptoms when children were infants and aged 3 and 5 years, child respiratory sinus arrythmia (RSA) reactivity (measure of PNS reactivity) at 3 years, and child internalizing symptoms at age 5 years. Consistent with an adaptive calibration perspective, analyses tested the roles of child RSA reactivity as both a mediator and a moderator of associations between maternal and child symptoms. Greater child RSA reactivity in response to a fearful video predicted higher internalizing symptoms among children exposed to higher levels of maternal depression or anxiety symptoms at age 5 years (moderation effects). Child RSA reactivity did not mediate relations between maternal depression or anxiety symptoms in infancy and child internalizing symptoms at age 5 years. The results suggest that heightened PNS reactivity may represent a biological vulnerability to stressful environments early in life: When coupled with maternal depression or anxiety exposure, child PNS reactivity may promote the development of internalizing psychopathology in early childhood.

Jiang P., Gao F., Liu S., Zhang S., Zhang X., Xia Z., Zhang W., Jiang T., Zhu J.L., Zhang Z., Shu Q., Snyder M., Li J.

Our current understanding of human physiology and activities is largely derived from sparse and discrete individual clinical measurements. To achieve precise, proactive, and effective health management of an individual, longitudinal, and dense tracking of personal physiomes and activities is required, which is only feasible by utilizing wearable biosensors. As a pilot study, we implemented a cloud computing infrastructure to integrate wearable sensors, mobile computing, digital signal processing, and machine learning to improve early detection of seizure onsets in children. We recruited 99 children diagnosed with epilepsy and longitudinally tracked them at single-second resolution using a wearable wristband, and prospectively acquired more than one billion data points. This unique dataset offered us an opportunity to quantify physiological dynamics (e.g., heart rate, stress response) across age groups and to identify physiological irregularities upon epilepsy onset. The high-dimensional personal physiome and activity profiles displayed a clustering pattern anchored by patient age groups. These signatory patterns included strong age and sex-specific effects on varying circadian rhythms and stress responses across major childhood developmental stages. For each patient, we further compared the physiological and activity profiles associated with seizure onsets with the personal baseline and developed a machine learning framework to accurately capture these onset moments. The performance of this framework was further replicated in another independent patient cohort. We next referenced our predictions with the electroencephalogram (EEG) signals on selected patients and demonstrated that our approach could detect subtle seizures not recognized by humans and could detect seizures prior to clinical onset. Our work demonstrated the feasibility of a real-time mobile infrastructure in a clinical setting, which has the potential to be valuable in caring for epileptic patients. Extension of such a system has the potential to be leveraged as a health management device or longitudinal phenotyping tool in clinical cohort studies.

Godleski S., Schuetze P., Eiden R.D., Nickerson A.B., Ostrov J.M.

Few studies have examined etiological pathways from prenatal substance exposure to adolescent reactive aggression. We tested a conceptual model that included hypothesized pathways from prenatal substance exposure to adolescent aggression via autonomic reactivity and violence exposure from infancy to early school age and maternal harshness across early childhood. The sample included 216 families (106 boys) who primarily self-identified as Black or Mixed Race. Results supported the hypothesized path from violence exposure across early childhood and early school age to school age autonomic reactivity and early adolescent reactive aggression. There was also a significant interaction effect of sympathetic and parasympathetic reactivity on adolescent reactive aggression, with sympathetic arousal and parasympathetic suppression at early school age associated with higher reactive relational and physical aggression in adolescence. Results emphasize the importance of early experiences and autonomic nervous system changes in contributing to the cascade of risk for reactive aggression in early adolescence. • Examined etiological pathways from prenatal substance exposure to adolescent reactive relational and physical aggression • Investigated child autonomic reactivity, maternal harshness, and violence exposure as potential predictors • Children with chronic violence exposure had more dysregulated autonomic reactivity and reactive aggression in adolescence • High sympathetic reactivity with parasympathetic withdrawal predicted higher reactive aggression in adolescence

Michalska K.J., Zhou E., Borelli J.L.

Individuals exhibit variability in the degree of correspondence between autonomic and subjective indicators of emotional experience. The current study examined whether convergence between autonomic arousal and negative emotions during emotion-inducing story vignettes is associated with internalizing symptoms in school-aged children. A diverse sample of 97 children aged 8 to 12 years participated in this study in which they reported on their anxiety and depression. Children's electrodermal activity was assessed while they read vignettes depicting children experiencing sadness and fear. Participants also reported on their emotional reaction to the vignettes. Children's anxiety and electrodermal activity to fear vignettes were associated only at high levels, but not mean or low levels, of self-reported negative emotions to fear vignettes. These findings suggest that hyperawareness, in which self-reported negative emotionality is high when physiological reactivity is also high, is associated with greater risk for anxiety, but not depression, during middle childhood.

Muscatello R.A., Pachol A., Romines A., Smith I., Corbett B.A.

Prolonged dysregulation of the autonomic nervous system (ANS) may increase propensity for physical or psychiatric illness. The current study examined differences in respiratory sinus arrhythmia (RSA) regulation in 215 adolescents with or without autism spectrum disorder (ASD) at Time 1 (T1; 10–13 years old) and 1 year later (Time 2; T2). Linear mixed effects models demonstrated lower RSA regulation in ASD, and a small interaction effect, showing blunted change in RSA from T1 to T2. Developmental differences in RSA regulation were particularly notable in females with ASD and those taking psychotropic medications. Results expand previous findings of reduced parasympathetic regulation in ASD by revealing a blunted developmental slope, indicating diagnostic differences may persist or worsen over time, particularly in females.

Rahal D., Alkon A., Shirtcliff E., Gonzales N., Fuligni A., Eskenazi B., Deardorff J.

We investigated whether parasympathetic and sympathetic nervous system (SNS) responses to social-evaluative threat at age 14 were related to the number of substances used between ages 14 and 16 among Mexican-origin adolescents (N = 243; 70.4% had never used substances by 14). Participants completed the Trier Social Stress Test, while cardiac measures of parasympathetic and SNS activity were measured continuously using respiratory sinus arrhythmia (RSA) and pre-ejection period (PEP), respectively. Participants reported whether they had ever used alcohol, marijuana, and cigarettes, and had ever vaped nicotine in their lifetime at ages 14 and 16. Multilevel models were used to test associations between RSA and PEP responses at age 14 and substance use at 16. Among youth who had not used substances by 14, dampened RSA and PEP responses, and profiles of greater coinhibition and lower reciprocal SNS activation between RSA and PEP, at age 14 were associated with using substances by 16. Among youth who used by 14, exaggerated PEP responses were associated with using more substances by age 16. Taken together, dampened autonomic responses to social-evaluative threat predicted initiation of substance use over two years, and difficulties with coordination of physiological responses may confer risk for substance use in adolescence.

Rudd K.L., Caron Z., Jones-Mason K., Coccia M., Conradt E., Alkon A., Bush N.R.

Across a range of challenging contexts, a complex system of stress responses within multiple domains (e.g., behavior, physiology) support, or thwart, an infant's capacity to navigate an ever-changing world. As understanding of these individual stress response systems has improved, researchers have called for integrated examinations across multiple systems and domains. However, extant research has usually focused on reactivity within a single system and very few explore the associations between multiple domains of stress responding. Drawing on a diverse sample of 135 mother-infant dyads, the current study explored biobehavioral concordance across the Autonomic Nervous System (ANS; measured via both cardiac and salivary indicators), Hypothalamic-pituitary-adrenal (HPA; measured via salivary indicators) axis, observed behavior, and maternal reports of temperament. Correlation analysis generally revealed moderate negative associations between ANS and HPA axis, moderate negative associations between ANS and coded negative behavior, and small-to-moderate positive associations between ANS and coded object engagement and social behavior. Salivary biomarkers and maternal report of infant temperament showed less concordance across systems and domains than cardiac ANS indicators. These findings provide a foundational understanding of the associations between biobehavioral indicators of stress responses in infancy, a period of high developmental plasticity.

Mastromatteo L.Y., Peruzza M., Scrimin S.

Self-regulation promotes engagement within the classroom. At a physiological level, a good indicator of the ability of the system to self-regulate is cardiac vagal tone (CVT).The present study aims to assess children's change over time (1 year) in their parasympathetic regulation (by way of CVT) in response to a social and cognitive stressor. Moreover, it addresses whether, if present, this change over time in regulation influences students' engagement in classroom activities while also accounting for classroom climate.Forty-nine second graders were assessed at two time points: November 2018 (T1) and 1 year later in 2019 (T2).Children's CVT was registered at rest and while performing a stressful task during which they were asked to cognitively perform while being socially evaluated. Children were also interviewed on how much they feel engaged in classroom activities and their perceptions of classroom climate.A repeated measures analysis of variance including 2 Time Points ×2 Phases of CVT Registration (baseline and during the stressful task) revealed a significant decrease in cardiac vagal activity from baseline to the task at T1, indicating that initially most children were not able to self-regulate and gave way to a stress response when facing the stressful task. The pattern changed at T2 when an active regulation took place signalled by an increase in CVT from baseline to the stressful task. Data analysis also revealed that among children who perceived a poorer classroom climate, the display of greater parasympathetic regulation over time was linked with higher active engagement in classroom activities.Growth in physiological regulation in response to a challenging task is associated with better engagement in classroom activities. Interventions and educational practice promoting the development of self-regulation strategies are recommended.

Zeytinoglu S., Calkins S.D., Leerkes E.M.

This study examined autonomic profiles in preschoolers (N = 278, age = 4.7 years) and their relations to self-regulation outcomes concurrently and one year later, in kindergarten. Children's sympathetic (preejection period [PEP]) and parasympathetic activity (respiratory sinus arrythmia [RSA]) were measured at rest and during cognitive and emotional tasks. Three self-regulatory competencies were assessed: executive functions, emotion regulation and behavioral regulation. Executive functioning was measured at ages 4 and 5 using laboratory tasks designed to assess updating/working memory, inhibitory control, and cognitive flexibility. Emotion regulation was observed during emotionally distressing tasks in the laboratory, both at ages 4 and 5. Behavioral regulation and emotional reactivity were assessed via teacher ratings in kindergarten, at age 5. Latent profile analysis yielded four autonomic profiles: moderate parasympathetic inhibition (45%), reciprocal sympathetic activation (26%), coinhibition (25%), and high sympathetic activation (7%). The reciprocal sympathetic activation group showed better executive functioning in preschool and kindergarten, particularly compared to the high sympathetic activation group. The moderate parasympathetic inhibition group showed lower emotional reactivity and better behavioral regulation in kindergarten, compared to the other three groups. Findings suggest that autonomic profiles meaningfully associate with self-regulation outcomes in early childhood, such that certain profiles relate to better self-regulation than others.

Fry C.M., Ram N., Gatzke-Kopp L.M.

Autonomic nervous system activity flexibly shifts and modulates behavior at multiple time scales, with some work suggesting that patterns of short-term reactivity contribute to long-term developmental change. However, previous work has largely considered sympathetic and parasympathetic systems independently, even though both systems contribute dynamically to the regulation of physiological arousal. Using physiological data obtained from 313 children in kindergarten, 1st, and 2nd grade we examined whether within-person autonomic coordination during an emotion-inducing film task in kindergarten was associated with developmental change in resting autonomic activity. On average, these kindergarteners exhibited reciprocal coordination during the approach-oriented emotion (angry, happy) condition and a lack of coordination during the avoidance-oriented emotion (fear, sad) condition. Alignment with these patterns was associated with more typical autonomic development, specifically an increase in resting respiratory sinus arrhythmia (RSA) and a decrease in resting skin conductance (SCR) from kindergarten to 2nd grade; while lack of coordination during the approach condition was associated with a relatively delayed increase in resting RSA and a steeper decline in SCR, and reciprocal coordination during the avoidance condition was associated with a lack of RSA increase. Findings highlight the need for additional consideration of how moment-to-moment dynamics of autonomic coordination influence longer-term development, and suggest that early patterns of atypical arousal may portend dysregulation of developing physiological systems.