Open Access

Science

, volume 370 , issue 6523 , pages 1473-1479

An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike

Michael Schoof ^{1, 2}

Bryan Faust ^{1, 2, 3, 4}

Reuben A Saunders ^{1, 5}

Smriti Sangwan ^{1, 2}

Veronica V Rezelj ⁶

Nick Hoppe ^{3, 4}

Morgane Boone ^{1, 2}

Christian B Billesbølle ^{3, 4}

Cristina Puchades ⁴

C. M. Azumaya ⁴

H. Kratochvil ⁴

Marcell Zimanyi ^{1, 2}

Ishan Deshpande ^{3, 4}

Jiahao Liang ³

Miles Sasha Dickinson ⁴

Kim Cuong Nguyen ⁴

Cynthia M Chio ⁴

Gregory E Merz ⁴

Michael C. Thompson ⁴

Devan Diwanji ⁴

Kaitlin Schaefer ⁴

Aditya A Anand ^{1, 2}

Niv Dobzinski ^{1, 2}

Beth S. Zha ⁷

Camille R. Simoneau ^{8, 9, 10}

Kristoffer E Leon ^{8, 9, 10}

Kris M White ^{11, 12}

Un Seng Chio ⁴

Meghna Gupta ⁴

M Jin ⁴

Fei Li ⁴

Yanxin Liu ⁴

Kaihua Zhang ⁴

David Bulkley ⁴

Ming Sun ⁴

Joel M Smith ⁴

Alexandrea Rizo ⁴

Frank R Moss ⁴

Axel F. Brilot ⁴

Sergei Pourmal ⁴

R Trenker ⁴

Thomas H Pospiech ⁴

Sayan Gupta ¹³

Benjamin Barsi Rhyne ³

Vladislav Belyy ^{1, 2}

Andrew W Barile Hill ¹⁴

Silke Nock ^{1, 2}

YUWEI LIU ^{1, 2}

Nevan J. Krogan ^{4, 5, 8, 9}

Corie Y. Ralston ¹³

Danielle Swaney ^{4, 5, 8, 9}

Adolfo García-Sastre ^{11, 12, 15, 16}

Melanie Ott ^{8, 9, 10}

Marco Vignuzzi ⁶

Peter Walter ^{1, 2}

Aashish Manglik ^{3, 4, 8, 17}

Hide authors affiliations Show authors affiliations: 17 affiliations

Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, USA.

University of California, San Francisco

Howard Hughes Medical Institute

Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA, USA. |

Department of Pharmaceutical Chemistry, University of California at San Francisco, San Francisco, CA, USA. |

⁴

Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA. |

⁵

Department of Cellular and Molecular Pharmacology, University of California at San Francisco, San Francisco, CA, USA. |

⁶

Viral Populations and Pathogenesis Unit, CNRS UMR 3569, Institut Pasteur, 75724 Paris Cedex 15, France. |

⁷

Department of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California San Francisco, San Francisco, CA, USA. |

⁸

Quantitative Biosciences Institute (QBI), University of California San Francisco, San Francisco, CA, USA. |

⁹

J. David Gladstone Institutes, San Francisco, CA, USA. |

¹⁰

Department of Medicine, University of California San Francisco, San Francisco, CA, USA. |

¹¹

Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. |

¹²

Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. |

¹³

Molecular Biophysics and Integrated Bioimaging and the Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. |

¹⁴

Cytiva Life Sciences, Marlborough, MA, USA. |

¹⁵

Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, NY, USA. |

¹⁶

The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. |

¹⁷

Department of Anesthesia and Perioperative Care, University of California at San Francisco, San Francisco, CA, USA. |

Publication type: Journal Article

Publication date: 2020-12-18

American Association for the Advancement of Science (AAAS)

Science

scimago Q1

wos Q1

SJR: 10.416

CiteScore: 48.4

Impact factor: 45.8

ISSN: 00368075, 10959203

DOI: 10.1126/science.abe3255

Copy DOI

PubMed ID: 33154106

Multidisciplinary

Abstract

Nanobodies that neutralize

Monoclonal antibodies that bind to the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) show therapeutic promise but must be produced in mammalian cells and need to be delivered intravenously. By contrast, single-domain antibodies called nanobodies can be produced in bacteria or yeast, and their stability may enable aerosol delivery. Two papers now report nanobodies that bind tightly to spike and efficiently neutralize SARS-CoV-2 in cells. Schoof et al. screened a yeast surface display of synthetic nanobodies and Xiang et al. screened anti-spike nanobodies produced by a llama. Both groups identified highly potent nanobodies that lock the spike protein in an inactive conformation. Multivalent constructs of selected nanobodies achieved even more potent neutralization.

Science , this issue p. 1473 , p. 1479

Found

2 citations

Dolzhikova Inna

🥼

PhD in Biological/biomedical sciences

54 publications, 2 910 citations

h-index: 15

N. F. Gamaleya National Research Center for Epidemiology and Microbiology of the Ministry of Health of the Russian Federation

D. I. Ivanovsky Institute of Virology

RNA drugs and vaccines

Virology

1 citation

Yarovaya Olga

PhD in Chemistry

117 publications, 1 788 citations, 14 reviews

h-index: 23

N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of the Russian Academy of Sciences

1 citation

Alekseev Alexander

74 publications, 650 citations, 7 reviews

h-index: 14

Dagestan State University

Federal Research Center of Fundamental and Translational Medicine

1 citation

Belenkaya Svetlana

PhD in Biological/biomedical sciences

39 publications, 263 citations

h-index: 9

State Research Center of Virology and Biotechnology VECTOR

N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of the Russian Academy of Sciences

1 citation

Xu H. Eric

PhD

725 publications, 21 120 citations, 8 reviews

h-index: 71

Shanghai Institute of Materia Medica, Chinese Academy of Sciences

1 citation

Nesmeyanova Valentina

18 publications, 123 citations

h-index: 6

State Research Center of Virology and Biotechnology VECTOR

Research interests

Biotechnology

Genetic engineering

1 citation

Kolosova Evgeniia

13 publications, 43 citations

h-index: 4

State Research Center of Virology and Biotechnology VECTOR

Altai State University

1 citation

Hossain M.

🥼 🤝

PhD in Engineering

282 publications, 11 909 citations, 628 reviews

h-index: 59

Bangladesh Atomic Energy Commission

1 citation

Shihab Uddin

🥼 🤝

PhD in Biological/biomedical sciences, senior lecturer, associate member of the Bangladesh Academy of Sciences

28 publications, 512 citations, 3 reviews

h-index: 12

King Fahd University of Petroleum and Minerals

Massachusetts Institute of Technology

Kyushu University

1 citation

Yamaguchi Daiki

8 publications, 78 citations, 1 review

h-index: 4

Kyoto Pharmaceutical University

Top-30

Journals

	2 4 6 8 10 12 14 16 18 20
bioRxiv	bioRxiv, 19, 5.08% bioRxiv 19 publications, 5.08%
Frontiers in Immunology	Frontiers in Immunology, 13, 3.48% Frontiers in Immunology 13 publications, 3.48%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 11, 2.94% International Journal of Molecular Sciences 11 publications, 2.94%
Nature Communications	Nature Communications, 9, 2.41% Nature Communications 9 publications, 2.41%
Proceedings of the National Academy of Sciences of the United States of America	Proceedings of the National Academy of Sciences of the United States of America, 7, 1.87% Proceedings of the National Academy of Sciences of the United States of America 7 publications, 1.87%
Viruses	Viruses, 5, 1.34% Viruses 5 publications, 1.34%
iScience	iScience, 5, 1.34% iScience 5 publications, 1.34%
International Journal of Biological Macromolecules	International Journal of Biological Macromolecules, 5, 1.34% International Journal of Biological Macromolecules 5 publications, 1.34%
Journal of Biological Chemistry	Journal of Biological Chemistry, 5, 1.34% Journal of Biological Chemistry 5 publications, 1.34%
Journal of Virology	Journal of Virology, 5, 1.34% Journal of Virology 5 publications, 1.34%
Journal of Chemical Information and Modeling	Journal of Chemical Information and Modeling, 4, 1.07% Journal of Chemical Information and Modeling 4 publications, 1.07%
ACS Synthetic Biology	ACS Synthetic Biology, 4, 1.07% ACS Synthetic Biology 4 publications, 1.07%
mAbs	mAbs, 4, 1.07% mAbs 4 publications, 1.07%
Microorganisms	Microorganisms, 3, 0.8% Microorganisms 3 publications, 0.8%
Scientific Reports	Scientific Reports, 3, 0.8% Scientific Reports 3 publications, 0.8%
Communications Biology	Communications Biology, 3, 0.8% Communications Biology 3 publications, 0.8%
Journal of Nanobiotechnology	Journal of Nanobiotechnology, 3, 0.8% Journal of Nanobiotechnology 3 publications, 0.8%
Cell	Cell, 3, 0.8% Cell 3 publications, 0.8%
Cell Reports	Cell Reports, 3, 0.8% Cell Reports 3 publications, 0.8%
Acta Pharmaceutica Sinica B	Acta Pharmaceutica Sinica B, 3, 0.8% Acta Pharmaceutica Sinica B 3 publications, 0.8%
Biochemistry	Biochemistry, 3, 0.8% Biochemistry 3 publications, 0.8%
Analytical Chemistry	Analytical Chemistry, 3, 0.8% Analytical Chemistry 3 publications, 0.8%
Nanoscale	Nanoscale, 3, 0.8% Nanoscale 3 publications, 0.8%
Science	Science, 3, 0.8% Science 3 publications, 0.8%
Science Translational Medicine	Science Translational Medicine, 3, 0.8% Science Translational Medicine 3 publications, 0.8%
Antiviral Research	Antiviral Research, 3, 0.8% Antiviral Research 3 publications, 0.8%
International Journal of Nanomedicine	International Journal of Nanomedicine, 2, 0.53% International Journal of Nanomedicine 2 publications, 0.53%
Vaccines	Vaccines, 2, 0.53% Vaccines 2 publications, 0.53%
Pathogens	Pathogens, 2, 0.53% Pathogens 2 publications, 0.53%
	2 4 6 8 10 12 14 16 18 20

Publishers

	10 20 30 40 50 60 70 80
Cold Spring Harbor Laboratory	Cold Spring Harbor Laboratory, 77, 20.59% Cold Spring Harbor Laboratory 77 publications, 20.59%
Elsevier	Elsevier, 64, 17.11% Elsevier 64 publications, 17.11%
Springer Nature	Springer Nature, 57, 15.24% Springer Nature 57 publications, 15.24%
MDPI	MDPI, 37, 9.89% MDPI 37 publications, 9.89%
American Chemical Society (ACS)	American Chemical Society (ACS), 28, 7.49% American Chemical Society (ACS) 28 publications, 7.49%
Wiley	Wiley, 18, 4.81% Wiley 18 publications, 4.81%
Frontiers Media S.A.	Frontiers Media S.A., 16, 4.28% Frontiers Media S.A. 16 publications, 4.28%
Taylor & Francis	Taylor & Francis, 10, 2.67% Taylor & Francis 10 publications, 2.67%
American Society for Microbiology	American Society for Microbiology, 10, 2.67% American Society for Microbiology 10 publications, 2.67%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 10, 2.67% Royal Society of Chemistry (RSC) 10 publications, 2.67%
American Association for the Advancement of Science (AAAS)	American Association for the Advancement of Science (AAAS), 9, 2.41% American Association for the Advancement of Science (AAAS) 9 publications, 2.41%
Oxford University Press	Oxford University Press, 8, 2.14% Oxford University Press 8 publications, 2.14%
Proceedings of the National Academy of Sciences (PNAS)	Proceedings of the National Academy of Sciences (PNAS), 7, 1.87% Proceedings of the National Academy of Sciences (PNAS) 7 publications, 1.87%
American Society for Biochemistry and Molecular Biology	American Society for Biochemistry and Molecular Biology, 5, 1.34% American Society for Biochemistry and Molecular Biology 5 publications, 1.34%
Public Library of Science (PLoS)	Public Library of Science (PLoS), 3, 0.8% Public Library of Science (PLoS) 3 publications, 0.8%
European Molecular Biology Organization	European Molecular Biology Organization, 2, 0.53% European Molecular Biology Organization 2 publications, 0.53%
eLife Sciences Publications	eLife Sciences Publications, 2, 0.53% eLife Sciences Publications 2 publications, 0.53%
Microbiology Society	Microbiology Society, 1, 0.27% Microbiology Society 1 publication, 0.27%
Cambridge University Press	Cambridge University Press, 1, 0.27% Cambridge University Press 1 publication, 0.27%
Rockefeller University Press	Rockefeller University Press, 1, 0.27% Rockefeller University Press 1 publication, 0.27%
Hindawi Limited	Hindawi Limited, 1, 0.27% Hindawi Limited 1 publication, 0.27%
The Company of Biologists	The Company of Biologists, 1, 0.27% The Company of Biologists 1 publication, 0.27%
The Royal Society	The Royal Society, 1, 0.27% The Royal Society 1 publication, 0.27%
Spandidos Publications	Spandidos Publications, 1, 0.27% Spandidos Publications 1 publication, 0.27%
Annual Reviews	Annual Reviews, 1, 0.27% Annual Reviews 1 publication, 0.27%
Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii	Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii, 1, 0.27% Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii 1 publication, 0.27%
The American Association of Immunologists	The American Association of Immunologists, 1, 0.27% The American Association of Immunologists 1 publication, 0.27%
Institute of Electrical and Electronics Engineers (IEEE)	Institute of Electrical and Electronics Engineers (IEEE), 1, 0.27% Institute of Electrical and Electronics Engineers (IEEE) 1 publication, 0.27%
	10 20 30 40 50 60 70 80

We do not take into account publications without a DOI.
Statistics recalculated weekly.

Are you a researcher?

Create a profile to get free access to personal recommendations for colleagues and new articles.

PDF

Metrics

375

Cite this

GOST |

Cite this

GOST Copy

Schoof M. et al. An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike // Science. 2020. Vol. 370. No. 6523. pp. 1473-1479.

GOST all authors (up to 50) Copy

Schoof M. et al. An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike // Science. 2020. Vol. 370. No. 6523. pp. 1473-1479.

RIS |

Cite this

RIS

BibTex |

Cite this

BibTex (up to 50 authors) Copy

@article{2020_Schoof,

author = {Michael Schoof and Bryan Faust and Reuben A Saunders and Smriti Sangwan and Veronica V Rezelj and Nick Hoppe and Morgane Boone and Christian B Billesbølle and Cristina Puchades and C. M. Azumaya and H. Kratochvil and Marcell Zimanyi and Ishan Deshpande and Jiahao Liang and Miles Sasha Dickinson and Kim Cuong Nguyen and Cynthia M Chio and Gregory E Merz and Michael C. Thompson and Devan Diwanji and Kaitlin Schaefer and Aditya A Anand and Niv Dobzinski and Beth S. Zha and Camille R. Simoneau and Kristoffer E Leon and Kris M White and Un Seng Chio and Meghna Gupta and M Jin and Fei Li and Yanxin Liu and Kaihua Zhang and David Bulkley and Ming Sun and Joel M Smith and Alexandrea Rizo and Frank R Moss and Axel F. Brilot and Sergei Pourmal and R Trenker and Thomas H Pospiech and Sayan Gupta and Benjamin Barsi Rhyne and Vladislav Belyy and Andrew W Barile Hill and Silke Nock and YUWEI LIU and Nevan J. Krogan and Corie Y. Ralston and others},

title = {An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike},

journal = {Science},

year = {2020},

volume = {370},

publisher = {American Association for the Advancement of Science (AAAS)},

month = {dec},

url = {https://doi.org/10.1126/science.abe3255},

number = {6523},

pages = {1473--1479},

doi = {10.1126/science.abe3255}

}

MLA

Cite this

MLA Copy

Schoof, Michael, et al. “An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike.” Science, vol. 370, no. 6523, Dec. 2020, pp. 1473-1479. https://doi.org/10.1126/science.abe3255.

Publisher

American Association for the Advancement of Science (AAAS)

Journal

Science

scimago Q1

wos Q1

SJR

10.416

CiteScore

48.4

Impact factor

45.8

ISSN

00368075 (Print)

10959203 (Electronic)