Open Access
Open access
American Society for Microbiology
Antimicrobial Agents and Chemotherapy
,  том 56 ,  издание 4 ,  страницы 1774-1783

Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors

Тип публикации: Journal Article
Дата публикации: 2012-04-01
American Society for Microbiology
scimago Q1
Tоп 10% SciMago
wos Q1
white level БС1
SJR: 1.431
CiteScore: 8.4
Impact factor: 4.5
ISSN: 00664804, 10986596
Pharmacology
Infectious Diseases
Pharmacology (medical)
Краткое описание
ABSTRACT

A reporter construct was created on the basis of the transcription attenuator region of the Escherichia coli tryptophan operon. Dual-fluorescent-protein genes for red fluorescent protein and cerulean fluorescent protein were used as a sensor and internal control of gene expression. The sequence of the attenuator was modified to avoid tryptophan sensitivity while preserving sensitivity to ribosome stalling. Antimicrobial compounds which cause translation arrest at the stage of elongation induce the reporter both in liquid culture and on an agar plate. This reporter could be used for high-throughput screening of translation inhibitors.

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Osterman I. A. et al. Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors // Antimicrobial Agents and Chemotherapy. 2012. Vol. 56. No. 4. pp. 1774-1783.
ГОСТ со всеми авторами (до 50) Скопировать
Osterman I. A., Prokhorova I. V., Sysoev V. O., Boykova Y. V., Efremenkova O. V., Svetlov M. S., Kolb V. A., Bogdanov A. A., Sergiev P. V., Dontsova O. A. Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors // Antimicrobial Agents and Chemotherapy. 2012. Vol. 56. No. 4. pp. 1774-1783.
RIS |
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RIS Скопировать
TY - JOUR
DO - 10.1128/AAC.05395-11
UR - https://journals.asm.org/doi/10.1128/AAC.05395-11
TI - Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors
T2 - Antimicrobial Agents and Chemotherapy
AU - Osterman, Ilya A.
AU - Prokhorova, Irina V
AU - Sysoev, Vasily O
AU - Boykova, Yulia V
AU - Efremenkova, Olga V
AU - Svetlov, Maxim S
AU - Kolb, Vyacheslav A.
AU - Bogdanov, Alexey A.
AU - Sergiev, Petr V.
AU - Dontsova, Olga A.
PY - 2012
DA - 2012/04/01
PB - American Society for Microbiology
SP - 1774-1783
IS - 4
VL - 56
PMID - 22252829
SN - 0066-4804
SN - 1098-6596
ER -
BibTex |
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@article{2012_Osterman,
author = {Ilya A. Osterman and Irina V Prokhorova and Vasily O Sysoev and Yulia V Boykova and Olga V Efremenkova and Maxim S Svetlov and Vyacheslav A. Kolb and Alexey A. Bogdanov and Petr V. Sergiev and Olga A. Dontsova},
title = {Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors},
journal = {Antimicrobial Agents and Chemotherapy},
year = {2012},
volume = {56},
publisher = {American Society for Microbiology},
month = {apr},
url = {https://journals.asm.org/doi/10.1128/AAC.05395-11},
number = {4},
pages = {1774--1783},
doi = {10.1128/AAC.05395-11}
}
MLA
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Osterman, Ilya A., et al. “Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors.” Antimicrobial Agents and Chemotherapy, vol. 56, no. 4, Apr. 2012, pp. 1774-1783. https://journals.asm.org/doi/10.1128/AAC.05395-11.
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