Open Access
Open access
Antimicrobial Agents and Chemotherapy, volume 56, issue 4, pages 1774-1783

Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors

Publication typeJournal Article
Publication date2012-04-01
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor4.9
ISSN00664804, 10986596
PubMed ID:  22252829
Pharmacology
Infectious Diseases
Pharmacology (medical)
Abstract
ABSTRACT

A reporter construct was created on the basis of the transcription attenuator region of theEscherichia colitryptophan operon. Dual-fluorescent-protein genes for red fluorescent protein and cerulean fluorescent protein were used as a sensor and internal control of gene expression. The sequence of the attenuator was modified to avoid tryptophan sensitivity while preserving sensitivity to ribosome stalling. Antimicrobial compounds which cause translation arrest at the stage of elongation induce the reporter both in liquid culture and on an agar plate. This reporter could be used for high-throughput screening of translation inhibitors.

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GOST |
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GOST Copy
Osterman I. A. et al. Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors // Antimicrobial Agents and Chemotherapy. 2012. Vol. 56. No. 4. pp. 1774-1783.
GOST all authors (up to 50) Copy
Osterman I. A., Prokhorova I. V., Sysoev V. O., Boykova Y. V., Efremenkova O. V., Svetlov M. S., Kolb V. A., Bogdanov A. A., Sergiev P. V., Dontsova O. A. Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors // Antimicrobial Agents and Chemotherapy. 2012. Vol. 56. No. 4. pp. 1774-1783.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1128/AAC.05395-11
UR - https://doi.org/10.1128%2FAAC.05395-11
TI - Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors
T2 - Antimicrobial Agents and Chemotherapy
AU - Osterman, Ilya A.
AU - Prokhorova, Irina V
AU - Sysoev, Vasily O
AU - Boykova, Yulia V
AU - Svetlov, Maxim S
AU - Kolb, Vyacheslav A.
AU - Sergiev, Petr V.
AU - Dontsova, Olga A.
AU - Efremenkova, Olga V
AU - Bogdanov, Alexey A.
PY - 2012
DA - 2012/04/01 00:00:00
PB - American Society for Microbiology
SP - 1774-1783
IS - 4
VL - 56
PMID - 22252829
SN - 0066-4804
SN - 1098-6596
ER -
BibTex |
Cite this
BibTex Copy
@article{2012_Osterman,
author = {Ilya A. Osterman and Irina V Prokhorova and Vasily O Sysoev and Yulia V Boykova and Maxim S Svetlov and Vyacheslav A. Kolb and Petr V. Sergiev and Olga A. Dontsova and Olga V Efremenkova and Alexey A. Bogdanov},
title = {Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors},
journal = {Antimicrobial Agents and Chemotherapy},
year = {2012},
volume = {56},
publisher = {American Society for Microbiology},
month = {apr},
url = {https://doi.org/10.1128%2FAAC.05395-11},
number = {4},
pages = {1774--1783},
doi = {10.1128/AAC.05395-11}
}
MLA
Cite this
MLA Copy
Osterman, Ilya A., et al. “Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors.” Antimicrobial Agents and Chemotherapy, vol. 56, no. 4, Apr. 2012, pp. 1774-1783. https://doi.org/10.1128%2FAAC.05395-11.
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