Open Access

Antimicrobial Agents and Chemotherapy

, volume 56 , issue 4 , pages 1774-1783

Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors

Ilya A. Osterman ¹

Irina V Prokhorova ¹

Vasily O Sysoev ¹

Yulia V Boykova ²

Olga V Efremenkova ²

Maxim S Svetlov ³

Vyacheslav A. Kolb ³

Alexey A. Bogdanov ¹

Petr V. Sergiev ¹

Olga A. Dontsova ¹

Hide authors affiliations Show authors affiliations: 3 affiliations

Department of Chemistry and A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia |

G. F. Gauze Institute for Search for New Antibiotics, Russian Academy of Medical Sciences, Moscow, Russia |

Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow region, Russia |

Publication type: Journal Article

Publication date: 2012-04-01

American Society for Microbiology

Antimicrobial Agents and Chemotherapy

scimago Q1

wos Q1

SJR: 1.431

CiteScore: 8.4

Impact factor: 4.5

ISSN: 00664804, 10986596

DOI: 10.1128/AAC.05395-11

Copy DOI

PubMed ID: 22252829

Pharmacology

Infectious Diseases

Pharmacology (medical)

Abstract

ABSTRACT

A reporter construct was created on the basis of the transcription attenuator region of the Escherichia coli tryptophan operon. Dual-fluorescent-protein genes for red fluorescent protein and cerulean fluorescent protein were used as a sensor and internal control of gene expression. The sequence of the attenuator was modified to avoid tryptophan sensitivity while preserving sensitivity to ribosome stalling. Antimicrobial compounds which cause translation arrest at the stage of elongation induce the reporter both in liquid culture and on an agar plate. This reporter could be used for high-throughput screening of translation inhibitors.

Found

23 citations

Sergiev Petr

DSc in Chemistry, professor

180 publications, 3 547 citations, 1 review

h-index: 35

Lomonosov Moscow State University

9 citations

Komarova Ekaterina

34 publications, 680 citations

h-index: 14

Lomonosov Moscow State University

Research interests

Molecular biology

4 citations

Rubtsova Maria

DSc in Chemistry

72 publications, 3 460 citations

h-index: 19

Lomonosov Moscow State University

4 citations

Skvortsov Dmitry

🥼

PhD in Chemistry

128 publications, 1 846 citations, 1 review

h-index: 26

Lomonosov Moscow State University

3 citations

Lukianov Dmitrii

47 publications, 545 citations

h-index: 12

Lomonosov Moscow State University

2 citations

Kabilov Marsel

PhD in Biological/biomedical sciences

177 publications, 2 196 citations, 7 reviews

h-index: 23

Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences

Research interests

Metagenomics

Transcriptomics

1 citation

Beloglazkina Elena

DSc in Chemistry, professor

296 publications, 3 248 citations, 11 reviews

h-index: 29

Lomonosov Moscow State University

1 citation

Borisov Roman

🤝

PhD in Chemistry

184 publications, 1 710 citations, 19 reviews

h-index: 20

A.V. Topchiev Institute of Petrochemical Synthesis RAS

Peoples' Friendship University of Russia

Research interests

Mass Spectrometry

1 citation

Razumova Elizaveta

🥼 🤝

7 publications, 94 citations

h-index: 4

Lomonosov Moscow State University

Research interests

Molecular biology

1 citation

Volynkina Inna

🥼

6 publications, 43 citations

h-index: 3

Lomonosov Moscow State University

Skolkovo Institute of Science and Technology

1 citation

Lyamzaev Konstantin

🥼 🤝

73 publications, 4 252 citations

h-index: 25

Pirogov Russian National Research Medical University

1 citation

Konevega Andrey

🥼 🤝

PhD in Physics and Mathematics

96 publications, 3 346 citations

h-index: 28

Peter the Great St. Petersburg Polytechnic University

National Research Centre "Kurchatov Institute"

Petersburg Nuclear Physics Institute of NRC «Kurchatov Institute»

Research interests

Antibiotics

Nuclear medicine

1 citation

Galyamina Mariya

22 publications, 222 citations

h-index: 9

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

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Cite this

GOST |

Cite this

GOST Copy

Osterman I. A. et al. Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors // Antimicrobial Agents and Chemotherapy. 2012. Vol. 56. No. 4. pp. 1774-1783.

GOST all authors (up to 50) Copy

Osterman I. A., Prokhorova I. V., Sysoev V. O., Boykova Y. V., Efremenkova O. V., Svetlov M. S., Kolb V. A., Bogdanov A. A., Sergiev P. V., Dontsova O. A. Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors // Antimicrobial Agents and Chemotherapy. 2012. Vol. 56. No. 4. pp. 1774-1783.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1128/AAC.05395-11

UR - https://journals.asm.org/doi/10.1128/AAC.05395-11

TI - Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors

T2 - Antimicrobial Agents and Chemotherapy

AU - Osterman, Ilya A.

AU - Prokhorova, Irina V

AU - Sysoev, Vasily O

AU - Boykova, Yulia V

AU - Efremenkova, Olga V

AU - Svetlov, Maxim S

AU - Kolb, Vyacheslav A.

AU - Bogdanov, Alexey A.

AU - Sergiev, Petr V.

AU - Dontsova, Olga A.

PY - 2012

DA - 2012/04/01

PB - American Society for Microbiology

SP - 1774-1783

IS - 4

VL - 56

PMID - 22252829

SN - 0066-4804

SN - 1098-6596

ER -

BibTex |

Cite this

BibTex (up to 50 authors) Copy

@article{2012_Osterman,

author = {Ilya A. Osterman and Irina V Prokhorova and Vasily O Sysoev and Yulia V Boykova and Olga V Efremenkova and Maxim S Svetlov and Vyacheslav A. Kolb and Alexey A. Bogdanov and Petr V. Sergiev and Olga A. Dontsova},

title = {Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors},

journal = {Antimicrobial Agents and Chemotherapy},

year = {2012},

volume = {56},

publisher = {American Society for Microbiology},

month = {apr},

url = {https://journals.asm.org/doi/10.1128/AAC.05395-11},

number = {4},

pages = {1774--1783},

doi = {10.1128/AAC.05395-11}

}

MLA

Cite this

MLA Copy

Osterman, Ilya A., et al. “Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors.” Antimicrobial Agents and Chemotherapy, vol. 56, no. 4, Apr. 2012, pp. 1774-1783. https://journals.asm.org/doi/10.1128/AAC.05395-11.

Publisher

American Society for Microbiology

Journal

Antimicrobial Agents and Chemotherapy

scimago Q1

wos Q1

SJR

1.431

CiteScore

8.4

Impact factor

4.5

ISSN

00664804 (Print)

10986596 (Electronic)

Labs

Laboratory of Nucleoprotein Chemistry, Department of Chemistry of Natural Compounds, Faculty of Chemistry

Profiles