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Doklady Biochemistry and Biophysics, volume 496, issue 1, pages 48-51

Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle

Singina G. N. 1
Sergiev P V 2, 3, 4
Lopukhov A. V. 1
Taradajnic N. P. 1
Ravin N. V. 5
Shedova E. N. 1
Taradajnic T. E. 1
Polejaeva I. A. 6
Dozev A V 1
Brem G 7
Dontsova O. A. 3, 4, 8, 9
ZINOVIEVA N.A. 1
Publication typeJournal Article
Publication date2021-03-10
Quartile SCImago
Q3
Quartile WOS
Q4
Impact factor0.8
ISSN16076729, 16083091
General Chemistry
Biochemistry
General Medicine
Biophysics
Abstract

Somatic Cell Nuclear Transfer (SCNT) technique was used to produce the first viable cloned cattle offspring in Russia. Whole-genome SNP genotyping confirmed that the cloned calf was identical to the fibroblast cell line that was used for SCNT. CRISPR/Cas9 approach was subsequently used to knock out genes for beta-lactoglobulin gene (PAEP) and the beta-lactoglobulin-like protein gene (LOC100848610) in the fibroblast cells. Gene editing (GE) efficiency was 4.4% for each of these genes. We successfully obtained single-cell-derived fibroblast colonies containing PAEP and LOC100848610 knockouts, which will be used to produce beta-lactoglobulin-deficient cattle.

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Singina G. N. et al. Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle // Doklady Biochemistry and Biophysics. 2021. Vol. 496. No. 1. pp. 48-51.
GOST all authors (up to 50) Copy
Singina G. N., Sergiev P. V., Lopukhov A. V., Rubtsova M. P., Taradajnic N. P., Ravin N. V., Shedova E. N., Taradajnic T. E., Polejaeva I. A., Dozev A. V., Brem G., Dontsova O. A., ZINOVIEVA N. Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle // Doklady Biochemistry and Biophysics. 2021. Vol. 496. No. 1. pp. 48-51.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1134/S1607672921010099
UR - https://doi.org/10.1134%2FS1607672921010099
TI - Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle
T2 - Doklady Biochemistry and Biophysics
AU - Singina, G. N.
AU - Sergiev, P V
AU - Lopukhov, A. V.
AU - Rubtsova, M. P.
AU - Taradajnic, N. P.
AU - Ravin, N. V.
AU - Shedova, E. N.
AU - Taradajnic, T. E.
AU - Polejaeva, I. A.
AU - Dozev, A V
AU - Brem, G
AU - Dontsova, O. A.
AU - ZINOVIEVA, N.A.
PY - 2021
DA - 2021/03/10 00:00:00
PB - Pleiades Publishing
SP - 48-51
IS - 1
VL - 496
PMID - 33689075
SN - 1607-6729
SN - 1608-3091
ER -
BibTex |
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BibTex Copy
@article{2021_Singina
author = {G. N. Singina and P V Sergiev and A. V. Lopukhov and M. P. Rubtsova and N. P. Taradajnic and N. V. Ravin and E. N. Shedova and T. E. Taradajnic and I. A. Polejaeva and A V Dozev and G Brem and O. A. Dontsova and N.A. ZINOVIEVA},
title = {Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle},
journal = {Doklady Biochemistry and Biophysics},
year = {2021},
volume = {496},
publisher = {Pleiades Publishing},
month = {mar},
url = {https://doi.org/10.1134%2FS1607672921010099},
number = {1},
pages = {48--51},
doi = {10.1134/S1607672921010099}
}
MLA
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MLA Copy
Singina, G. N., et al. “Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle.” Doklady Biochemistry and Biophysics, vol. 496, no. 1, Mar. 2021, pp. 48-51. https://doi.org/10.1134%2FS1607672921010099.
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