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Doklady Biochemistry and Biophysics, том 496, издание 1, номера страниц: 48-51

Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle

Singina G. N. 1
Sergiev P. V. 2, 3, 4
Lopukhov A. V. 1
Taradajnic N. P. 1
Ravin N. V. 5
Shedova E. N. 1
Taradajnic T. E. 1
Polejaeva I. A. 6
Dozev A. V. 1
Brem G. 7
Dontsova O. A. 3, 4, 8, 9
Zinovieva N. A. 1
1
 
Ernst Federal Science Center for Animal Husbandry, Podolsk, Russia
5
 
Research Center of Biotechnology, Moscow, Russia
6
 
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, USA
7
 
Department of Animal Breeding and Genetics, University of Veterinary Medicine, Vienna, Austria
Тип документаJournal Article
Дата публикации2021-03-10
ИздательPleiades Publishing
Название журналаDoklady Biochemistry and Biophysics
Квартиль по SCImagoQ3
Квартиль по Web of ScienceQ4
Импакт-фактор 20210.83
ISSN16076729, 16083091
General Chemistry
Biochemistry
General Medicine
Biophysics
Краткое описание

Somatic Cell Nuclear Transfer (SCNT) technique was used to produce the first viable cloned cattle offspring in Russia. Whole-genome SNP genotyping confirmed that the cloned calf was identical to the fibroblast cell line that was used for SCNT. CRISPR/Cas9 approach was subsequently used to knock out genes for beta-lactoglobulin gene (PAEP) and the beta-lactoglobulin-like protein gene (LOC100848610) in the fibroblast cells. Gene editing (GE) efficiency was 4.4% for each of these genes. We successfully obtained single-cell-derived fibroblast colonies containing PAEP and LOC100848610 knockouts, which will be used to produce beta-lactoglobulin-deficient cattle.

Пристатейные ссылки: 7
Цитируется в публикациях: 2
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Q2 Journal of Biotechnology 2006 цитирований: 49
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1. Singina G.N. и др. Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle // Doklady Biochemistry and Biophysics. 2021. Т. 496. № 1. С. 48–51.
RIS |
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TY - JOUR

DO - 10.1134/s1607672921010099

UR - http://dx.doi.org/10.1134/S1607672921010099

TI - Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle

T2 - Doklady Biochemistry and Biophysics

AU - Singina, G. N.

AU - Sergiev, P. V.

AU - Lopukhov, A. V.

AU - Rubtsova, M. P.

AU - Taradajnic, N. P.

AU - Ravin, N. V.

AU - Shedova, E. N.

AU - Taradajnic, T. E.

AU - Polejaeva, I. A.

AU - Dozev, A. V.

AU - Brem, G.

AU - Dontsova, O. A.

AU - Zinovieva, N. A.

PY - 2021

DA - 2021/03/10

PB - Pleiades Publishing Ltd

SP - 48-51

IS - 1

VL - 496

SN - 1607-6729

SN - 1608-3091

ER -

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@article{2021,

doi = {10.1134/s1607672921010099},

url = {https://doi.org/10.1134%2Fs1607672921010099},

year = 2021,

month = {mar},

publisher = {Pleiades Publishing Ltd},

volume = {496},

number = {1},

pages = {48--51},

author = {G. N. Singina and P. V. Sergiev and A. V. Lopukhov and M. P. Rubtsova and N. P. Taradajnic and N. V. Ravin and E. N. Shedova and T. E. Taradajnic and I. A. Polejaeva and A. V. Dozev and G. Brem and O. A. Dontsova and N. A. Zinovieva},

title = {Production of a Cloned Offspring and {CRISPR}/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle}

}

MLA
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Singina, G. N. et al. “Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle.” Doklady Biochemistry and Biophysics 496.1 (2021): 48–51. Crossref. Web.