Open Access
Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle
Тип публикации: Journal Article
Дата публикации: 2021-03-10
scimago Q3
wos Q4
БС3
SJR: 0.223
CiteScore: 1.5
Impact factor: 0.7
ISSN: 16076729, 16083091
PubMed ID:
33689075
General Chemistry
Biochemistry
General Medicine
Biophysics
Краткое описание
Somatic Cell Nuclear Transfer (SCNT) technique was used to produce the first viable cloned cattle offspring in Russia. Whole-genome SNP genotyping confirmed that the cloned calf was identical to the fibroblast cell line that was used for SCNT. CRISPR/Cas9 approach was subsequently used to knock out genes for beta-lactoglobulin gene (PAEP) and the beta-lactoglobulin-like protein gene (LOC100848610) in the fibroblast cells. Gene editing (GE) efficiency was 4.4% for each of these genes. We successfully obtained single-cell-derived fibroblast colonies containing PAEP and LOC100848610 knockouts, which will be used to produce beta-lactoglobulin-deficient cattle.
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Singina G. N. et al. Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle // Doklady Biochemistry and Biophysics. 2021. Vol. 496. No. 1. pp. 48-51.
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Singina G. N., Sergiev P. V., Lopukhov A. V., Rubtsova M. P., Taradajnic N. P., Ravin N. V., Shedova E. N., Taradajnic T. E., Polejaeva I. A., Dozev A. V., Brem G., Dontsova O. A., ZINOVIEVA N. Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle // Doklady Biochemistry and Biophysics. 2021. Vol. 496. No. 1. pp. 48-51.
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TY - JOUR
DO - 10.1134/S1607672921010099
UR - http://link.springer.com/10.1134/S1607672921010099
TI - Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle
T2 - Doklady Biochemistry and Biophysics
AU - Singina, G. N.
AU - Sergiev, P V
AU - Lopukhov, A. V.
AU - Rubtsova, M. P.
AU - Taradajnic, N. P.
AU - Ravin, N. V.
AU - Shedova, E. N.
AU - Taradajnic, T. E.
AU - Polejaeva, I. A.
AU - Dozev, A V
AU - Brem, G
AU - Dontsova, O. A.
AU - ZINOVIEVA, N.A.
PY - 2021
DA - 2021/03/10
PB - Pleiades Publishing
SP - 48-51
IS - 1
VL - 496
PMID - 33689075
SN - 1607-6729
SN - 1608-3091
ER -
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BibTex (до 50 авторов)
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@article{2021_Singina,
author = {G. N. Singina and P V Sergiev and A. V. Lopukhov and M. P. Rubtsova and N. P. Taradajnic and N. V. Ravin and E. N. Shedova and T. E. Taradajnic and I. A. Polejaeva and A V Dozev and G Brem and O. A. Dontsova and N.A. ZINOVIEVA},
title = {Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle},
journal = {Doklady Biochemistry and Biophysics},
year = {2021},
volume = {496},
publisher = {Pleiades Publishing},
month = {mar},
url = {http://link.springer.com/10.1134/S1607672921010099},
number = {1},
pages = {48--51},
doi = {10.1134/S1607672921010099}
}
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MLA
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Singina, G. N., et al. “Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle.” Doklady Biochemistry and Biophysics, vol. 496, no. 1, Mar. 2021, pp. 48-51. http://link.springer.com/10.1134/S1607672921010099.