Open Access
Agronomy, volume 11, issue 2, pages 394
Suspension Cell Culture of Dioscorea deltoidea—A Renewable Source of Biomass and Furostanol Glycosides for Food and Pharmaceutical Industry
Titova Maria V.
1
,
Popova Elena V.
1
,
Konstantinova Svetlana V
2
,
Kochkin Dmitry V.
1, 2
,
Ivanov Igor M
1
,
Klyushin Andrey G
1
,
Titova Elena G
3
,
Nebera Elena A
3
,
Tolmacheva Galina S
4
,
Kotenkova Elena
4
,
Nosov Alexandr M
1, 2
,
Paek Kee Yoeup
5
1
3
All-Russian State Research Institute for Control Standardization and Certification of Veterinary Preparations, 123022 Moscow, Russia
|
5
Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju 361-763, Korea
|
Publication type: Journal Article
Publication date: 2021-02-23
Agronomy and Crop Science
Abstract
Dioscorea deltoidea is a medicinal plant valued for its high content of steroidal glycosides (SG)—bioactive compounds with cardioprotective and immunomodulation actions, also used to treat reproductive system disorders. To overcome the limitations of natural resources of this species, a suspension cell culture of D. deltoidea was developed as a renewable and ecologically sustainable source of raw biomass and SG. Cell culture demonstrated stable and intensive growth in the laboratory (20 L) and industrial (630 L) bioreactors operated under a semi-continuous regime (specific growth rate 0.11–1.12 day−1, growth index 3.5–3.7). Maximum dry weight accumulation (8.5–8.8 g/L) and SG content (47–57 mg/g DW) were recorded during the stationary phase. Bioreactor-produced cell biomass contained inorganic macro (K, Ca, Mg, Na) and micro (Zn, Mn, Fe, B, Al, Cu, Cr, Se, Co, Ni) elements in concentrations within the safe range of dietary recommendations. Acute toxicity test showed no or insignificant changes in organ weight, hematological panel and blood biochemistry of laboratory animals fed with 2000 and 5000 mg/kg dry biomass. The results suggest that cell culture of D. deltoidea grown in bioreactors has great potential to be used as functional foods and a component of specialized dietary supplements in complex therapy of reproductive system disorders and mineral deficiency.
Citations by journals
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1
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3
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Nutrients
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1 publication, 7.14%
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1 publication, 7.14%
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Critical Reviews in Biotechnology
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1 publication, 7.14%
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Industrial Crops and Products
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1 publication, 7.14%
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Reference Series in Phytochemistry
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1 publication, 7.14%
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1 publication, 7.14%
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1
2
3
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Citations by publishers
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1
2
3
4
5
6
7
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
7 publications, 50%
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Springer Nature
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Springer Nature
2 publications, 14.29%
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Center of Pharmaceutical Analytics Ltd
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Center of Pharmaceutical Analytics Ltd
1 publication, 7.14%
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Taylor & Francis
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Taylor & Francis
1 publication, 7.14%
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Elsevier
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Elsevier
1 publication, 7.14%
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Frontiers Media S.A.
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Frontiers Media S.A.
1 publication, 7.14%
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Research Square Platform LLC
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Research Square Platform LLC, 1, 7.14%
Research Square Platform LLC
1 publication, 7.14%
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1
2
3
4
5
6
7
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Titova M. V. et al. Suspension Cell Culture of Dioscorea deltoidea—A Renewable Source of Biomass and Furostanol Glycosides for Food and Pharmaceutical Industry // Agronomy. 2021. Vol. 11. No. 2. p. 394.
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Titova M. V., Popova E. V., Konstantinova S. V., Kochkin D. V., Ivanov I. M., Klyushin A. G., Titova E. G., Nebera E. A., Vasilevskaya E., Tolmacheva G. S., Kotenkova E., Nosov A. M., Paek K. Y. Suspension Cell Culture of Dioscorea deltoidea—A Renewable Source of Biomass and Furostanol Glycosides for Food and Pharmaceutical Industry // Agronomy. 2021. Vol. 11. No. 2. p. 394.
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TY - JOUR
DO - 10.3390/agronomy11020394
UR - https://doi.org/10.3390%2Fagronomy11020394
TI - Suspension Cell Culture of Dioscorea deltoidea—A Renewable Source of Biomass and Furostanol Glycosides for Food and Pharmaceutical Industry
T2 - Agronomy
AU - Titova, Maria V.
AU - Popova, Elena V.
AU - Konstantinova, Svetlana V
AU - Kochkin, Dmitry V.
AU - Ivanov, Igor M
AU - Klyushin, Andrey G
AU - Titova, Elena G
AU - Nebera, Elena A
AU - Tolmacheva, Galina S
AU - Nosov, Alexandr M
AU - Paek, Kee Yoeup
AU - Vasilevskaya, Ekaterina
AU - Kotenkova, Elena
PY - 2021
DA - 2021/02/23 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 394
IS - 2
VL - 11
SN - 2073-4395
ER -
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@article{2021_Titova,
author = {Maria V. Titova and Elena V. Popova and Svetlana V Konstantinova and Dmitry V. Kochkin and Igor M Ivanov and Andrey G Klyushin and Elena G Titova and Elena A Nebera and Galina S Tolmacheva and Alexandr M Nosov and Kee Yoeup Paek and Ekaterina Vasilevskaya and Elena Kotenkova},
title = {Suspension Cell Culture of Dioscorea deltoidea—A Renewable Source of Biomass and Furostanol Glycosides for Food and Pharmaceutical Industry},
journal = {Agronomy},
year = {2021},
volume = {11},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {feb},
url = {https://doi.org/10.3390%2Fagronomy11020394},
number = {2},
pages = {394},
doi = {10.3390/agronomy11020394}
}
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Titova, Maria V., et al. “Suspension Cell Culture of Dioscorea deltoidea—A Renewable Source of Biomass and Furostanol Glycosides for Food and Pharmaceutical Industry.” Agronomy, vol. 11, no. 2, Feb. 2021, p. 394. https://doi.org/10.3390%2Fagronomy11020394.