Open Access
International Journal of Molecular Sciences, volume 23, issue 23, pages 14828
Uptake and Metabolization of Serotonin by Granulosa Cells Form a Functional Barrier in the Mouse Ovary
Publication type: Journal Article
Publication date: 2022-11-27
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 5.6
ISSN: 16616596, 14220067
PubMed ID:
36499156
Catalysis
Organic Chemistry
Inorganic Chemistry
Physical and Theoretical Chemistry
Computer Science Applications
Spectroscopy
Molecular Biology
General Medicine
Abstract
Serotonin (5-HT) plays an essential role in regulating female reproductive function in many animals. 5-HT accumulates in the mammalian ovary with the involvement of membrane serotonin transporter SERT and is functionally active in the oocytes of growing follicles, but shows almost no activity in follicular cells. In this study, we clarified the interplay between 5-HT membrane transport and its degradation by monoamine oxidase (MAO) in the mammalian ovary. Using pharmacologic agents and immunohistochemical staining of the cryosections of ovaries after serotonin administration in vitro, we demonstrated the activity of transport and degradation systems in ovarian follicles. The MAO inhibitor pargyline increased serotonin accumulation in the granulosa cells of growing follicles, indicating the activity of both serotonin uptake and degradation by MAO in these cells. The activity of MAO and the specificity of the membrane transport of serotonin was confirmed in primary granulosa cell culture treated with pargyline and fluoxetine. Moreover, the accumulation of serotonin is more effective in the denuded oocytes and occurs at lower concentrations than in the oocytes within the follicles. This confirms that the activity of SERT and MAO in the granulosa cells surrounding the oocytes impedes the accumulation of serotonin in the oocytes and forms a functional barrier to serotonin.
Citations by journals
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1
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Biology
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Biology
1 publication, 33.33%
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International Journal of Molecular Sciences
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International Journal of Molecular Sciences
1 publication, 33.33%
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Russian Journal of Developmental Biology
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Russian Journal of Developmental Biology
1 publication, 33.33%
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1
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Citations by publishers
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2
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 66.67%
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Pleiades Publishing
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Pleiades Publishing
1 publication, 33.33%
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1
2
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Alyoshina N. M. et al. Uptake and Metabolization of Serotonin by Granulosa Cells Form a Functional Barrier in the Mouse Ovary // International Journal of Molecular Sciences. 2022. Vol. 23. No. 23. p. 14828.
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Alyoshina N. M., Tkachenko M. D., Malchenko L. A., Shmukler Y. B., Nikishin D. A. Uptake and Metabolization of Serotonin by Granulosa Cells Form a Functional Barrier in the Mouse Ovary // International Journal of Molecular Sciences. 2022. Vol. 23. No. 23. p. 14828.
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TY - JOUR
DO - 10.3390/ijms232314828
UR - https://doi.org/10.3390%2Fijms232314828
TI - Uptake and Metabolization of Serotonin by Granulosa Cells Form a Functional Barrier in the Mouse Ovary
T2 - International Journal of Molecular Sciences
AU - Alyoshina, Nina M
AU - Tkachenko, Maria D
AU - Malchenko, Lyudmila A
AU - Shmukler, Yuri B.
AU - Nikishin, Denis A
PY - 2022
DA - 2022/11/27 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 14828
IS - 23
VL - 23
PMID - 36499156
SN - 1661-6596
SN - 1422-0067
ER -
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@article{2022_Alyoshina,
author = {Nina M Alyoshina and Maria D Tkachenko and Lyudmila A Malchenko and Yuri B. Shmukler and Denis A Nikishin},
title = {Uptake and Metabolization of Serotonin by Granulosa Cells Form a Functional Barrier in the Mouse Ovary},
journal = {International Journal of Molecular Sciences},
year = {2022},
volume = {23},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {nov},
url = {https://doi.org/10.3390%2Fijms232314828},
number = {23},
pages = {14828},
doi = {10.3390/ijms232314828}
}
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MLA
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Alyoshina, Nina M., et al. “Uptake and Metabolization of Serotonin by Granulosa Cells Form a Functional Barrier in the Mouse Ovary.” International Journal of Molecular Sciences, vol. 23, no. 23, Nov. 2022, p. 14828. https://doi.org/10.3390%2Fijms232314828.