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volume 27 issue 15 pages 4981

Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach

Matheus Mikio Takeyama 1
Márcia Corrêa De Carvalho 1
Helena Sacco Carvalho 2
Cristiane Rodrigues Silva 1
ANA PAULA TROVATTI UETANABARO 3
Andrea Miura Da Costa 3
Joseph A Medeiros Evaristo 4
Fábio César Sousa Nogueira 4, 5
Maria Gabriela Bello Koblitz 1
Publication typeJournal Article
Publication date2022-08-05
scimago Q1
wos Q2
SJR0.865
CiteScore8.6
Impact factor4.6
ISSN14203049
Organic Chemistry
Drug Discovery
Physical and Theoretical Chemistry
Pharmaceutical Science
Molecular Medicine
Analytical Chemistry
Chemistry (miscellaneous)
Abstract

A sequential design strategy was applied to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain, from Brazilian sugarcane liquor vat, on passion fruit residue flour (PFRF), through solid-state fermentation (SSF). A factorial design was performed to determine the influence variables and two rotational central composite designs were executed. The validated experimental result was of 7.1 U mL−1 using 50% PFRF (w/w), pH 5, 30 °C for 24 h, under static SSF. Polygalacturonase, pectin methyl esterase, pectin–lyase and pectate–lyase activities were 3.5; 0.08; 3.1 and 0.8 U mL−1, respectively. Shotgun proteomics analysis of the crude extract enabled the identification of two pectin–lyases, one pectate–lyase and a glucosidase. The crude enzymatic extract maintained at least 80% of its original activity at pH values and temperatures ranging from 2 to 8 and 30 to 80 °C, respectively, over 60 min incubation. Results revealed that PFRF might be a cost-effective and eco-friendly substrate to produce pectinases. Statistical optimization led to fermentation conditions wherein pectin active proteins predominated. To the extent of our knowledge, this is the first study reporting the synthesis of pectate lyase by S. cerevisiae.

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Takeyama M. M. et al. Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach // Molecules. 2022. Vol. 27. No. 15. p. 4981.
GOST all authors (up to 50) Copy
Takeyama M. M., De Carvalho M. C., Carvalho H. S., Silva C. R., UETANABARO A. P. T., Da Costa A. M., Evaristo J. A. M., Nogueira F. C. S., Fai A. E. C., Koblitz M. G. B. Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach // Molecules. 2022. Vol. 27. No. 15. p. 4981.
RIS |
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RIS Copy
TY - JOUR
DO - 10.3390/molecules27154981
UR - https://doi.org/10.3390/molecules27154981
TI - Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach
T2 - Molecules
AU - Takeyama, Matheus Mikio
AU - De Carvalho, Márcia Corrêa
AU - Carvalho, Helena Sacco
AU - Silva, Cristiane Rodrigues
AU - UETANABARO, ANA PAULA TROVATTI
AU - Da Costa, Andrea Miura
AU - Evaristo, Joseph A Medeiros
AU - Nogueira, Fábio César Sousa
AU - Fai, Ana Elizabeth Cavalcante
AU - Koblitz, Maria Gabriela Bello
PY - 2022
DA - 2022/08/05
PB - MDPI
SP - 4981
IS - 15
VL - 27
PMID - 35956930
SN - 1420-3049
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2022_Takeyama,
author = {Matheus Mikio Takeyama and Márcia Corrêa De Carvalho and Helena Sacco Carvalho and Cristiane Rodrigues Silva and ANA PAULA TROVATTI UETANABARO and Andrea Miura Da Costa and Joseph A Medeiros Evaristo and Fábio César Sousa Nogueira and Ana Elizabeth Cavalcante Fai and Maria Gabriela Bello Koblitz},
title = {Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach},
journal = {Molecules},
year = {2022},
volume = {27},
publisher = {MDPI},
month = {aug},
url = {https://doi.org/10.3390/molecules27154981},
number = {15},
pages = {4981},
doi = {10.3390/molecules27154981}
}
MLA
Cite this
MLA Copy
Takeyama, Matheus Mikio, et al. “Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach.” Molecules, vol. 27, no. 15, Aug. 2022, p. 4981. https://doi.org/10.3390/molecules27154981.