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Antibiotics, том 8, издание 4, номер публикации: 236

Exploitation of potentially new antibiotics from mangrove actinobacteria in maowei sea by combination of multiple discovery strategies

Lu Qin-Pei 1, 2
Ye Jing-Jing 3
Huang Yong-Mei 4
Liu Di 5
Liu Li-Fang 1, 2
Dong Kun 6
Osterman Ilya A. 7, 8
Sergiev Petr V. 7, 8
Dontsova Olga A. 7, 8, 9
Jia Shu-Han 3
Huang Da-lin 3
Department of Microbial Chemistry, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
Beijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
College of Basic Medical Sciences, Guilin Medical University, Guilin 541004, China
Zhanjiang for R&D Marine Microbial Resources in the Beibu Gulf Rim, Marine Biomedical Research Institute, Guangdong Medical University, Zhanjiang 524023, China
College of Life Sciences, Jiamusi University, Jiamusi 154007, China
College of Life Science and Technology, China Pharmaceutical University, Nanjing 211198, China
Тип документаJournal Article
Дата публикации2019-11-27
ИздательMultidisciplinary Digital Publishing Institute (MDPI)
Название журналаAntibiotics
Квартиль по SCImagoQ1
Квартиль по Web of ScienceQ1
Импакт-фактор 20215.22
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)
General Pharmacology, Toxicology and Pharmaceutics
Краткое описание

Rediscovery of known antibiotics from actinobacteria, especially Streptomyces, has become a bottleneck issue. Nowadays, more specific identification and dereplication could be acquired by a combination of modern analytic techniques with various databases. In this study, 261 actinobacterial strains were isolated from 8 mangrove soil samples by culture-dependent method. A total of 83 strains were selected to evaluate antibacterial activities and mechanisms by disc diffusion method and a unique double fluorescent protein reporter system (pDualrep2), respectively. Thirty-two strains exhibited antagonistic activity against at least one of the “ESKAPE” pathogens. Four Streptomyces strains (B475, B486, B353, and B98) showed strong inhibitory activity against Gram-positive bacteria and induced DNA damage SOS response. One Micromonospora strain (B704) exhibited inhibitory activity against several pathogens and induced attenuation-based translational inhibitors reporter. Seven members of quinoxaline-type antibiotics including quinomycin A, quinomycin monosulfoxide, and other five putative new analogues were found from the culture broth of strain B475 by a combination of anti-MRSA guide, HPTLC, HPLC-UV, and UPLC-UV-HRESIMS/MS analysis, Chemspider searching, and MS/MS-based molecular networking analysis. In conclusion, this study not only demonstrated that mangrove is a rich source of actinobacteria with the potentially new antibiotics but showed rapid dereplication of known antibiotics in the early stage can improve efficiency for the discovery of new antibiotics.

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1. Lu Q.-P. и др. Exploitation of Potentially New Antibiotics from Mangrove Actinobacteria in Maowei Sea by Combination of Multiple Discovery Strategies // Antibiotics. 2019. Т. 8. № 4. С. 236.


DO - 10.3390/antibiotics8040236

UR -

TI - Exploitation of Potentially New Antibiotics from Mangrove Actinobacteria in Maowei Sea by Combination of Multiple Discovery Strategies

T2 - Antibiotics

AU - Lu, Qin-Pei

AU - Ye, Jing-Jing

AU - Huang, Yong-Mei

AU - Liu, Di

AU - Liu, Li-Fang

AU - Dong, Kun

AU - Razumova, Elizaveta A.

AU - Osterman, Ilya A.

AU - Sergiev, Petr V.

AU - Dontsova, Olga A.

AU - Jia, Shu-Han

AU - Huang, Da-Lin

AU - Sun, Cheng-Hang

PY - 2019

DA - 2019/11/27


SP - 236

IS - 4

VL - 8

SN - 2079-6382

ER -

BibTex |


doi = {10.3390/antibiotics8040236},

url = {},

year = 2019,

month = {nov},

publisher = {{MDPI} {AG}},

volume = {8},

number = {4},

pages = {236},

author = {Qin-Pei Lu and Jing-Jing Ye and Yong-Mei Huang and Di Liu and Li-Fang Liu and Kun Dong and Elizaveta A. Razumova and Ilya A. Osterman and Petr V. Sergiev and Olga A. Dontsova and Shu-Han Jia and Da-Lin Huang and Cheng-Hang Sun},

title = {Exploitation of Potentially New Antibiotics from Mangrove Actinobacteria in Maowei Sea by Combination of Multiple Discovery Strategies}


Lu, Qin-Pei et al. “Exploitation of Potentially New Antibiotics from Mangrove Actinobacteria in Maowei Sea by Combination of Multiple Discovery Strategies.” Antibiotics 8.4 (2019): 236. Crossref. Web.