Open Access
Antibiotics, volume 8, issue 4, pages 236
Exploitation of potentially new antibiotics from mangrove actinobacteria in maowei sea by combination of multiple discovery strategies
Lu Qin-Pei
1, 2
,
Ye Jing Jing
3
,
Huang Yong-Mei
4
,
Liu Di
5
,
Liu Li-Fang
1, 2
,
Dong Kun
6
,
Osterman Ilya A.
7, 8
,
Sergiev Petr V.
7, 8
,
Dontsova Olga A.
7, 8, 9
,
Jia Shu Han
3
,
Huang Da-Lin
3
,
Sun Chenghang
1, 2
3
College of Basic Medical Sciences, Guilin Medical University, Guilin 541004, China
|
Publication type: Journal Article
Publication date: 2019-11-27
PubMed ID:
31783564
Biochemistry
Microbiology (medical)
Microbiology
Infectious Diseases
Pharmacology (medical)
General Pharmacology, Toxicology and Pharmaceutics
Abstract
Rediscovery of known antibiotics from actinobacteria, especially Streptomyces, has become a bottleneck issue. Nowadays, more specific identification and dereplication could be acquired by a combination of modern analytic techniques with various databases. In this study, 261 actinobacterial strains were isolated from 8 mangrove soil samples by culture-dependent method. A total of 83 strains were selected to evaluate antibacterial activities and mechanisms by disc diffusion method and a unique double fluorescent protein reporter system (pDualrep2), respectively. Thirty-two strains exhibited antagonistic activity against at least one of the “ESKAPE” pathogens. Four Streptomyces strains (B475, B486, B353, and B98) showed strong inhibitory activity against Gram-positive bacteria and induced DNA damage SOS response. One Micromonospora strain (B704) exhibited inhibitory activity against several pathogens and induced attenuation-based translational inhibitors reporter. Seven members of quinoxaline-type antibiotics including quinomycin A, quinomycin monosulfoxide, and other five putative new analogues were found from the culture broth of strain B475 by a combination of anti-MRSA guide, HPTLC, HPLC-UV, and UPLC-UV-HRESIMS/MS analysis, Chemspider searching, and MS/MS-based molecular networking analysis. In conclusion, this study not only demonstrated that mangrove is a rich source of actinobacteria with the potentially new antibiotics but showed rapid dereplication of known antibiotics in the early stage can improve efficiency for the discovery of new antibiotics.
Citations by journals
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1
2
3
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Frontiers in Microbiology
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Frontiers in Microbiology
3 publications, 25%
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Marine Drugs
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Marine Drugs
3 publications, 25%
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Molecules
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Molecules
1 publication, 8.33%
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Pharmaceuticals
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Pharmaceuticals
1 publication, 8.33%
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Journal of Infection and Public Health
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Journal of Infection and Public Health
1 publication, 8.33%
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1
2
3
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Citations by publishers
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1
2
3
4
5
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
5 publications, 41.67%
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Frontiers Media S.A.
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Frontiers Media S.A.
3 publications, 25%
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Elsevier
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Elsevier
1 publication, 8.33%
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1
2
3
4
5
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Lu Q. et al. Exploitation of potentially new antibiotics from mangrove actinobacteria in maowei sea by combination of multiple discovery strategies // Antibiotics. 2019. Vol. 8. No. 4. p. 236.
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Lu Q., Ye J. J., Huang Y., Liu D., Liu L., Dong K., Razumova E. A., Osterman I. A., Sergiev P. V., Dontsova O. A., Jia S. H., Huang D., Sun C. Exploitation of potentially new antibiotics from mangrove actinobacteria in maowei sea by combination of multiple discovery strategies // Antibiotics. 2019. Vol. 8. No. 4. p. 236.
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TY - JOUR
DO - 10.3390/antibiotics8040236
UR - https://doi.org/10.3390%2Fantibiotics8040236
TI - Exploitation of potentially new antibiotics from mangrove actinobacteria in maowei sea by combination of multiple discovery strategies
T2 - Antibiotics
AU - Lu, Qin-Pei
AU - Ye, Jing Jing
AU - Huang, Yong-Mei
AU - Liu, Li-Fang
AU - Dong, Kun
AU - Razumova, Elizaveta A
AU - Osterman, Ilya A.
AU - Sergiev, Petr V.
AU - Dontsova, Olga A.
AU - Jia, Shu Han
AU - Huang, Da-Lin
AU - Liu, Di
AU - Sun, Chenghang
PY - 2019
DA - 2019/11/27 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 236
IS - 4
VL - 8
PMID - 31783564
SN - 2079-6382
ER -
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@article{2019_Lu,
author = {Qin-Pei Lu and Jing Jing Ye and Yong-Mei Huang and Li-Fang Liu and Kun Dong and Elizaveta A Razumova and Ilya A. Osterman and Petr V. Sergiev and Olga A. Dontsova and Shu Han Jia and Da-Lin Huang and Di Liu and Chenghang Sun},
title = {Exploitation of potentially new antibiotics from mangrove actinobacteria in maowei sea by combination of multiple discovery strategies},
journal = {Antibiotics},
year = {2019},
volume = {8},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {nov},
url = {https://doi.org/10.3390%2Fantibiotics8040236},
number = {4},
pages = {236},
doi = {10.3390/antibiotics8040236}
}
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MLA
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Lu, Qin-Pei, et al. “Exploitation of potentially new antibiotics from mangrove actinobacteria in maowei sea by combination of multiple discovery strategies.” Antibiotics, vol. 8, no. 4, Nov. 2019, p. 236. https://doi.org/10.3390%2Fantibiotics8040236.