Open Access
Microorganisms, volume 8, issue 5, pages 680
Baikalomycins A-C, New Aquayamycin-Type Angucyclines Isolated from Lake Baikal Derived Streptomyces sp. IB201691-2A
Voitsekhovskaia Irina
1
,
Paulus Constanze
2
,
Dahlem Charlotte
3
,
Rebets Yuriy
4
,
Nadmid Suvd
4
,
Zapp Josef
3
,
Axenov-Gribanov Denis V
1, 5
,
Rückert Christian
6
,
TIMOFEYEV MAXIM A.
1, 5
,
Kalinowski Jörn
6
,
Luzhetskyy Andriy
2, 4
2
Helmholtz Institute for Pharmaceutical Research Saarland, 66123 Saarbrücken, Germany
|
3
Pharmaceutical Biology, Saarland University, 66123 Saarbrücken, Germany
|
4
Pharmaceutical Biotechnology, Saarland University, 66123 Saarbrücken, Germany
|
5
Baikal Research Centre, 664003 Irkutsk, Russia
|
6
Technology Platform Genomics, Center for Biotechnology (CeBiTec), Bielefeld University, 33615 Bielefeld, Germany
|
Publication type: Journal Article
Publication date: 2020-05-07
PubMed ID:
32392775
Microbiology (medical)
Microbiology
Virology
Abstract
Natural products produced by bacteria found in unusual and poorly studied ecosystems, such as Lake Baikal, represent a promising source of new valuable drug leads. Here we report the isolation of a new Streptomyces sp. strain IB201691-2A from the Lake Baikal endemic mollusk Benedictia baicalensis. In the course of an activity guided screening three new angucyclines, named baikalomycins A–C, were isolated and characterized, highlighting the potential of poorly investigated ecological niches. Besides that, the strain was found to accumulate large quantities of rabelomycin and 5-hydroxy-rabelomycin, known shunt products in angucyclines biosynthesis. Baikalomycins A–C demonstrated varying degrees of anticancer activity. Rabelomycin and 5-hydroxy-rabelomycin further demonstrated antiproliferative activities. The structure elucidation showed that baikalomycin A is a modified aquayamycin with β-d-amicetose and two additional hydroxyl groups at unusual positions (6a and 12a) of aglycone. Baikalomycins B and C have alternating second sugars attached, α-l-amicetose and α-l-aculose, respectively. The gene cluster for baikalomycins biosynthesis was identified by genome mining, cloned using a transformation-associated recombination technique and successfully expressed in S. albus J1074. It contains a typical set of genes responsible for an angucycline core assembly, all necessary genes for the deoxy sugars biosynthesis, and three genes coding for the glycosyltransferase enzymes. Heterologous expression and deletion experiments allowed to assign the function of glycosyltransferases involved in the decoration of baikalomycins aglycone.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Voitsekhovskaia I. et al. Baikalomycins A-C, New Aquayamycin-Type Angucyclines Isolated from Lake Baikal Derived Streptomyces sp. IB201691-2A // Microorganisms. 2020. Vol. 8. No. 5. p. 680.
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Voitsekhovskaia I., Paulus C., Dahlem C., Rebets Y., Nadmid S., Zapp J., Axenov-Gribanov D. V., Rückert C., TIMOFEYEV M. A., Kalinowski J., Kiemer A. K., Luzhetskyy A. Baikalomycins A-C, New Aquayamycin-Type Angucyclines Isolated from Lake Baikal Derived Streptomyces sp. IB201691-2A // Microorganisms. 2020. Vol. 8. No. 5. p. 680.
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TY - JOUR
DO - 10.3390/microorganisms8050680
UR - https://doi.org/10.3390%2Fmicroorganisms8050680
TI - Baikalomycins A-C, New Aquayamycin-Type Angucyclines Isolated from Lake Baikal Derived Streptomyces sp. IB201691-2A
T2 - Microorganisms
AU - Voitsekhovskaia, Irina
AU - Paulus, Constanze
AU - Rebets, Yuriy
AU - Nadmid, Suvd
AU - Zapp, Josef
AU - Rückert, Christian
AU - Kalinowski, Jörn
AU - Luzhetskyy, Andriy
AU - Dahlem, Charlotte
AU - Axenov-Gribanov, Denis V
AU - TIMOFEYEV, MAXIM A.
AU - Kiemer, Alexandra K
PY - 2020
DA - 2020/05/07 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 680
IS - 5
VL - 8
PMID - 32392775
SN - 2076-2607
ER -
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@article{2020_Voitsekhovskaia,
author = {Irina Voitsekhovskaia and Constanze Paulus and Yuriy Rebets and Suvd Nadmid and Josef Zapp and Christian Rückert and Jörn Kalinowski and Andriy Luzhetskyy and Charlotte Dahlem and Denis V Axenov-Gribanov and MAXIM A. TIMOFEYEV and Alexandra K Kiemer},
title = {Baikalomycins A-C, New Aquayamycin-Type Angucyclines Isolated from Lake Baikal Derived Streptomyces sp. IB201691-2A},
journal = {Microorganisms},
year = {2020},
volume = {8},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {may},
url = {https://doi.org/10.3390%2Fmicroorganisms8050680},
number = {5},
pages = {680},
doi = {10.3390/microorganisms8050680}
}
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Voitsekhovskaia, Irina, et al. “Baikalomycins A-C, New Aquayamycin-Type Angucyclines Isolated from Lake Baikal Derived Streptomyces sp. IB201691-2A.” Microorganisms, vol. 8, no. 5, May. 2020, p. 680. https://doi.org/10.3390%2Fmicroorganisms8050680.