Hetiamacin E and F, New amicoumacin antibiotics from bacillus subtilis PJS using MS/MS-based molecular networking
To combat escalating levels of antibiotic resistance, novel strategies are developed to address the everlasting demand for new antibiotics. This study aimed at investigating amicoumacin antibiotics from the desert-derived Bacillus subtilis PJS by using the modern MS/MS-based molecular networking approach. Two new amicoumacins, namely hetiamacin E (1) and hetiamacin F (2), were finally isolated. The planar structures were determined by analysis of extensive NMR spectroscopic and HR–ESI–MS data, and the absolute configurations were concluded by analysis of the CD spectrum. Hetiamacin E (1) showed strong antibacterial activities against methicillin-sensitive and resistant Staphylococcus epidermidis at 2–4 µg/mL, and methicillin-sensitive and resistant Staphylococcus aureus at 8–16 µg/mL. Hetiamacin F (2) exhibited moderate antibacterial activities against Staphylococcus sp. at 32 µg/mL. Both compounds were inhibitors of protein biosynthesis demonstrated by a double fluorescent protein reporter system.
TY - JOUR
DO - 10.3390/molecules25194446
UR - http://dx.doi.org/10.3390/molecules25194446
TI - Hetiamacin E and F, New Amicoumacin Antibiotics from Bacillus subtilis PJS Using MS/MS-Based Molecular Networking
T2 - Molecules
AU - Wang, Ting
AU - Lu, Qinpei
AU - Sun, Chenghang
AU - Lukianov, Dmitrii
AU - Osterman, Ilya Andreevich
AU - Sergiev, Petr Vladimirovich
AU - Dontsova, Olga Anatolievna
AU - Hu, Xinxin
AU - You, Xuefu
AU - Liu, Shaowei
AU - Wu, Gang
PY - 2020
DA - 2020/09/27
PB - MDPI AG
SP - 4446
IS - 19
VL - 25
SN - 1420-3049
ER -
@article{2020,
doi = {10.3390/molecules25194446},
url = {https://doi.org/10.3390%2Fmolecules25194446},
year = 2020,
month = {sep},
publisher = {{MDPI} {AG}},
volume = {25},
number = {19},
pages = {4446},
author = {Ting Wang and Qinpei Lu and Chenghang Sun and Dmitrii Lukianov and Ilya Andreevich Osterman and Petr Vladimirovich Sergiev and Olga Anatolievna Dontsova and Xinxin Hu and Xuefu You and Shaowei Liu and Gang Wu},
title = {Hetiamacin E and F, New Amicoumacin Antibiotics from Bacillus subtilis {PJS} Using {MS}/{MS}-Based Molecular Networking}
}