Open Access
Nucleic Acids Research, volume 46, issue 3, pages 1525-1540
Structure and function of the N-terminal domain of the yeast telomerase reverse transcriptase
Petrova Olga A
1
,
Rodina Elena V.
3
,
Efimov Sergey
4
,
Hackenberg Claudia
5
,
Hakanpää Johanna
5
,
Klochkov V V
4
,
Lebedev Andrej A
6
,
Chugunova Anastasia A
7, 8
,
Malyavko Alexander N
7, 8
,
Zatsepin Timofei S.
7, 8
,
Zvereva Maria
3
,
Lamzin V S
5
,
Dontsova Olga A.
7, 8
,
5
European Molecular Biology Laboratory, Notkestrasse 85, 22607 Hamburg, Germany
|
6
Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Didcot, UK.
|
Publication type: Journal Article
Publication date: 2017-12-23
Journal:
Nucleic Acids Research
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 14.9
ISSN: 03051048, 13624962
PubMed ID:
29294091
Genetics
Abstract
Abstract The elongation of single-stranded DNA repeats at the 3′-ends of chromosomes by telomerase is a key process in maintaining genome integrity in eukaryotes. Abnormal activation of telomerase leads to uncontrolled cell division, whereas its down-regulation is attributed to ageing and several pathologies related to early cell death. Telomerase function is based on the dynamic interactions of its catalytic subunit (TERT) with nucleic acids—telomerase RNA, telomeric DNA and the DNA/RNA heteroduplex. Here, we present the crystallographic and NMR structures of the N-terminal (TEN) domain of TERT from the thermotolerant yeast Hansenula polymorpha and demonstrate the structural conservation of the core motif in evolutionarily divergent organisms. We identify the TEN residues that are involved in interactions with the telomerase RNA and in the recognition of the ‘fork’ at the distal end of the DNA product/RNA template heteroduplex. We propose that the TEN domain assists telomerase biological function and is involved in restricting the size of the heteroduplex during telomere repeat synthesis.
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1
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Petrova O. A. et al. Structure and function of the N-terminal domain of the yeast telomerase reverse transcriptase // Nucleic Acids Research. 2017. Vol. 46. No. 3. pp. 1525-1540.
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Petrova O. A., Mantsyzov A. B., Rodina E. V., Efimov S., Hackenberg C., Hakanpää J., Klochkov V. V., Lebedev A. A., Chugunova A. A., Malyavko A. N., Zatsepin T. S., Mishin A. S., Zvereva M., Lamzin V. S., Dontsova O. A., Polshakov V. I. Structure and function of the N-terminal domain of the yeast telomerase reverse transcriptase // Nucleic Acids Research. 2017. Vol. 46. No. 3. pp. 1525-1540.
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TY - JOUR
DO - 10.1093/nar/gkx1275
UR - https://doi.org/10.1093%2Fnar%2Fgkx1275
TI - Structure and function of the N-terminal domain of the yeast telomerase reverse transcriptase
T2 - Nucleic Acids Research
AU - Petrova, Olga A
AU - Rodina, Elena V.
AU - Hackenberg, Claudia
AU - Hakanpää, Johanna
AU - Lebedev, Andrej A
AU - Chugunova, Anastasia A
AU - Dontsova, Olga A.
AU - Polshakov, Vladimir I.
AU - Malyavko, Alexander N
AU - Mantsyzov, Alexey B.
AU - Efimov, Sergey
AU - Klochkov, V V
AU - Zatsepin, Timofei S.
AU - Mishin, Alexander S.
AU - Zvereva, Maria
AU - Lamzin, V S
PY - 2017
DA - 2017/12/23 00:00:00
PB - Oxford University Press
SP - 1525-1540
IS - 3
VL - 46
PMID - 29294091
SN - 0305-1048
SN - 1362-4962
ER -
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@article{2017_Petrova,
author = {Olga A Petrova and Elena V. Rodina and Claudia Hackenberg and Johanna Hakanpää and Andrej A Lebedev and Anastasia A Chugunova and Olga A. Dontsova and Vladimir I. Polshakov and Alexander N Malyavko and Alexey B. Mantsyzov and Sergey Efimov and V V Klochkov and Timofei S. Zatsepin and Alexander S. Mishin and Maria Zvereva and V S Lamzin},
title = {Structure and function of the N-terminal domain of the yeast telomerase reverse transcriptase},
journal = {Nucleic Acids Research},
year = {2017},
volume = {46},
publisher = {Oxford University Press},
month = {dec},
url = {https://doi.org/10.1093%2Fnar%2Fgkx1275},
number = {3},
pages = {1525--1540},
doi = {10.1093/nar/gkx1275}
}
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MLA
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Petrova, Olga A., et al. “Structure and function of the N-terminal domain of the yeast telomerase reverse transcriptase.” Nucleic Acids Research, vol. 46, no. 3, Dec. 2017, pp. 1525-1540. https://doi.org/10.1093%2Fnar%2Fgkx1275.