Heterotypic 3D models of human breast and brain tumors for the study of tumor-stroma interaction and the development of new therapeutic approaches
Publications
36
Citations
352
h-index
12
Authorization required.
A project to study the interactions of tumor cells and the microenvironment in response to immunotherapy using 3D cellular models of human breast and brain tumors.
- Cell and tissue culture
- In vitro tumor models: primary and conventional tumor cell cultures, tissue spheroids
- Confocal microscopy
- Western blot
- Immunohistochemistry (IHC)
- Immunocytochemistry
- Methods for assessing the viability and growth of cell and tissue cultures
- Microscopy
- Assessment of cytotoxic activity (MTT test, Cell-TitreGlo, Rezazurin, Cell/Death kit)
- Flow-through cell sorting
- Flow cytometry
- Work with mammalian cell cultures, transfection
- Mathematical modeling
Research directions
Heterotypic 3D models of human breast and brain tumors for the study of tumor-stroma interaction and the development of new therapeutic approaches
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Heterogeneous microenvironment, including breast tumors and glioblastomas, affects the progression and drug resistance of the tumor. Immunotherapy with oncolytic viruses(s) and immune cells is a promising treatment strategy. Heterotypic 3D models in vitro will allow detailed investigation of the interactions of tumor cells and its microenvironment and will serve as a platform for testing effective therapeutic approaches based on VV-GMCSF-Lac and NK cells.
Heterotypic 3D breast cancer Model
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A model of tumor and stromal cells as a tool for evaluating the sensitization of tumor cells and microenvironment cells to immunotherapy by standard chemotherapy drugs.
The influence of various types of tumor-associated fibroblasts in tumor progression and the formation of an immunosuppressive microenvironment.
Publications and patents
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Partners
Lab address
Олимпийский просп., 1, Сочи, Краснодарский край
Authorization required.