Acharya N. G. Ranga Agricultural University

Mahanti N.K., Pandiselvam R., Kothakota A., Ishwarya S. P., Chakraborty S.K., Kumar M., Cozzolino D.

Fruits are vulnerable to mechanical damages and physiological disorders caused by the static and dynamic forces acting on them during transportation and abiotic stresses throughout their growth and development, respectively. Identifying these defects is central to quality monitoring in the fruit processing industry. Conventionally, industries employ manual separation to segregate damaged fruits in the processing line. However, manual sorting is laborious, time-consuming, skilled labor-intensive, and destructive. Besides, it is incapable of inspecting every fruit on a fast-moving conveyor belt. Therefore, industries are looking for rapid, non-destructive, and precise technologies for the online inspection of every fruit in the process line. Non-destructive techniques (NDTs) such as biospeckle, X-ray imaging, hyperspectral imaging (HSI), and thermal imaging (TI) involve noninvasive testing of sample surfaces. Earlier review articles have emphasized the applications of various NDTs in determining fruit quality and safety, but with limited focus on image processing and analysis. Therefore, this review focuses on the working principle of these NDTs in detecting fruit damages, their instrumentation, and the steps involved in image processing and analysis. The final sections highlight the limitations and future prospects pertaining to each technique. Biospeckle, HSI, and TI techniques can detect surface damages due to their limited light penetration depth. The HSI spectrum is useful in detecting the defects and fruit quality parameters. Active TI can detect even minor damages in the fruit, but it is not appropriate for industrial production lines. Conversely, X-ray imaging can detect fruit internal damages. The synergistic applications of these NDTs along with appropriate chemometric procedures are useful in identifying damaged fruits without human interference and evade their entry into the processing line. • Advanced non-destructive techniques (NDTs) for fruit damage detection are reviewed. • Principle of image acquisition, processing, and analysis are elaborated. • Case-studies are presented on fruit damage detection by non-destructive techniques. • Limitations and future scope of NDTs forfruit damage detection are discussed.

Mallareddy M., Thirumalaikumar R., Balasubramanian P., Naseeruddin R., Nithya N., Mariadoss A., Eazhilkrishna N., Choudhary A.K., Deiveegan M., Subramanian E., Padmaja B., Vijayakumar S.

Rice is a water-guzzling crop cultivated mostly through inefficient irrigation methods which leads to low water use efficiency and many environmental problems. Additionally, the export of virtual water through rice trading and the looming water crisis poses significant threats to the sustainability of rice production and food security. There are several alternative rice production methods to improve water use efficiency. These include aerobic rice, direct-seeded rice (DSR), alternate wetting and drying (AWD), saturated soil culture (SSC), drip-irrigated rice, a system of rice intensification (SRI), and smart irrigation with sensors and the Internet of Things (IoT). However, each method has its own advantages and disadvantages. For example, drip-irrigated rice and IoT-based automated irrigation are not feasible for poor farmers due to the high production costs associated with specialized machinery and tools. Similarly, aerobic rice, drip-irrigated rice, and the SRI are labor-intensive, making them unsuitable for areas with a shortage of labor. On the other hand, DSR is suitable for labor-scarce areas, provided herbicides are used to control weeds. In this article, the suitability of different water-saving rice production methods is reviewed based on factors such as climate, soil type, labor, energy, and greenhouse gas emissions, and their prospects and challenges are evaluated. Additionally, the article examines how cultural practices, such as seed treatment, weed control, and nutrition management, contribute to enhancing water use efficiency in rice production.

Roychowdhury R., Das S.P., Gupta A., Parihar P., Chandrasekhar K., Sarker U., Kumar A., Ramrao D.P., Sudhakar C.

The present day’s ongoing global warming and climate change adversely affect plants through imposing environmental (abiotic) stresses and disease pressure. The major abiotic factors such as drought, heat, cold, salinity, etc., hamper a plant’s innate growth and development, resulting in reduced yield and quality, with the possibility of undesired traits. In the 21st century, the advent of high-throughput sequencing tools, state-of-the-art biotechnological techniques and bioinformatic analyzing pipelines led to the easy characterization of plant traits for abiotic stress response and tolerance mechanisms by applying the ‘omics’ toolbox. Panomics pipeline including genomics, transcriptomics, proteomics, metabolomics, epigenomics, proteogenomics, interactomics, ionomics, phenomics, etc., have become very handy nowadays. This is important to produce climate-smart future crops with a proper understanding of the molecular mechanisms of abiotic stress responses by the plant’s genes, transcripts, proteins, epigenome, cellular metabolic circuits and resultant phenotype. Instead of mono-omics, two or more (hence ‘multi-omics’) integrated-omics approaches can decipher the plant’s abiotic stress tolerance response very well. Multi-omics-characterized plants can be used as potent genetic resources to incorporate into the future breeding program. For the practical utility of crop improvement, multi-omics approaches for particular abiotic stress tolerance can be combined with genome-assisted breeding (GAB) by being pyramided with improved crop yield, food quality and associated agronomic traits and can open a new era of omics-assisted breeding. Thus, multi-omics pipelines together are able to decipher molecular processes, biomarkers, targets for genetic engineering, regulatory networks and precision agriculture solutions for a crop’s variable abiotic stress tolerance to ensure food security under changing environmental circumstances.

Alam M., Alam S., Shamsi A., Adnan M., Elasbali A.M., Al-Soud W.A., Alreshidi M., Hawsawi Y.M., Tippana A., Pasupuleti V.R., Hassan M.I.

Non-small cell lung carcinoma (NSCLC) comprises 80%–85% of lung cancer cases. EGFR is involved in several cancer developments, including NSCLC. The EGFR pathway regulates the Bax/Bcl-2 cascade in NSCLC. Increasing understanding of the molecular mechanisms of fundamental tumor progression has guided the development of numerous antitumor drugs. The development and improvement of rationally planned inhibitors and agents targeting particular cellular and biological pathways in cancer have been signified as a most important paradigm shift in the strategy to treat and manage lung cancer. Newer approaches and novel chemotherapeutic agents are required to accompany present cancer therapies for improving efficiency. Using natural products as a drug with an effective delivery system may benefit therapeutics. Naturally originated compounds such as phytochemicals provide crucial sources for novel agents/drugs and resources for tumor therapy. Applying the small-molecule inhibitors (SMIs)/phytochemicals has led to potent preclinical discoveries in various human tumor preclinical models, including lung cancer. In this review, we summarize recent information on the molecular mechanisms of the Bax/Bcl-2 cascade and EGFR pathway in NSCLC and target them for therapeutic implications. We further described the therapeutic potential of Bax/Bcl-2/EGFR SMIs, mainly those with more potent and selectivity, including gefitinib, EGCG, ABT-737, thymoquinone, quercetin, and venetoclax. In addition, we explained the targeting EGFR pathway and ongoing in vitro and in vivo and clinical investigations in NSCLC. Exploration of such inhibitors facilitates the future treatment and management of NSCLC.

Jamaloddin M., Durga Rani C.V., Swathi G., Anuradha C., Vanisri S., Rajan C.P., Krishnam Raju S., Bhuvaneshwari V., Jagadeeswar R., Laha G.S., Prasad M.S., Satyanarayana P.V., Cheralu C., Rajani G., Ramprasad E., et. al.

Bacterial blight (BB) and fungal blast diseases are the major biotic constraints that limit rice productivity. To sustain yield improvement in rice, it is necessary to developed yield potential of the rice varieties by incorporation of biotic stress resistance genes. Tellahamsa is a well-adapted popular high yielding rice variety in Telangana state, India. However, the variety is highly susceptible to BB and blast. In this study, simultaneous stepwise transfer of genes through marker-assisted backcross breeding (MABB) strategy was used to introgress two major BB (Xa21 and xa13) and two major blast resistance genes (Pi54 and Pi1) into Tellahamsa. In each generation (from F1 to ICF3) foreground selection was done using gene-specific markers viz., pTA248 (Xa21), xa13prom (xa13), Pi54MAS (Pi54) and RM224 (Pi1). Two independent BC2F1 lines of Tellahamsa/ISM (Cross-I) and Tellahamsa/NLR145 (Cross-II) possessing 92% and 94% recurrent parent genome (RPG) respectively were intercrossed to develop ICF1—ICF3 generations. These gene pyramided lines were evaluated for key agro-morphological traits, quality, and resistance against blast at three different hotspot locations as well as BB at two locations. Two ICF3 gene pyramided lines viz., TH-625-159 and TH-625-491 possessing four genes exhibited a high level of resistance to BB and blast. In the future, these improved Tellahamsa lines could be developed as mega varieties for different agro-climatic zones and also as potential donors for different pre-breeding rice research.

Kolahalam L.A., Prasad K.R., Murali Krishna P., Supraja N.

Abstract The plant extracts are known for their anti-inflammatory, antifungal, antiviral and antibacterial properties. The use of plant extracts in the preparation of bio-materials increases their biological application. In this concern, herein reporting an eco-friendly procedure which is also a simple and cost effective, for the synthesis of Zinc Oxide nanoparticles (ZnONPs) using Saussurea lappa plant root (rhizome) extract as a fuel. The prepared nanoparticles were confirmed using various characterization techniques. The Dynamic light scattering data showed 123.5 nm particle size with -99.9 mv zeta potential which indicates excellent stability of the particles. The peak at 541 cm−1 in the IR spectrum is assigned to the stretching frequency of the zinc-binding to oxygen. The X-ray diffraction peaks confirm the close association with JCPDS Data Card No: 36-1451. The FESEM data revealed a hexagonal wurtzite structure with a hexagonal shape of synthesized ZnO nanoparticles. The antibacterial studies indicate the gram-negative strains showed better inhibition activity than gram-positive strains. Among Fungal strains, Aspergillus niger and flavus, Fusarium oxysporum, and Rhizopus oryzae showed good inhibition activity at higher concentrations. The cytotoxic data indicates the 5 μg/mL of the ZnO particles showed cytotoxicity on the CHO cell line and with IC50 value 3.164 ± 0.8956 μg/mL.

Najeeb S., Ali J., Mahender A., Pang Y.L., Zilhas J., Murugaiyan V., Vemireddy L.R., Li Z.

An attempt was made in the current study to identify the main-effect and co-localized quantitative trait loci (QTLs) for germination and early seedling growth traits under low-temperature stress (LTS) conditions in rice. The plant material used in this study was an early backcross population of 230 introgression lines (ILs) in BCIF7 generation derived from the Weed Tolerant Rice-1 (WTR-1) (as the recipient) and Haoannong (HNG) (as the donor). Genetic analyses of LTS tolerance revealed a total of 27 main-effect quantitative trait loci (M-QTLs) mapped on 12 chromosomes. These QTLs explained more than 10% of phenotypic variance (PV), and average PV of 12.71% while employing 704 high-quality SNP markers. Of these 27 QTLs distributed on 12 chromosomes, 11 were associated with low-temperature germination (LTG), nine with low-temperature germination stress index (LTGS), five with root length stress index (RLSI), and two with biomass stress index (BMSI) QTLs, shoot length stress index (SLSI) and root length stress index (RLSI), seven with seed vigor index (SVI), and single QTL with root length (RL). Among them, five significant major QTLs (qLTG(I)1, qLTGS(I)1–2, qLTG(I)5, qLTGS(I)5, and qLTG(I)7) mapped on chromosomes 1, 5, and 7 were associated with LTG and LTGS traits and the PV explained ranged from 16 to 23.3%. The genomic regions of these QTLs were co-localized with two to six QTLs. Most of the QTLs were growth stage-specific and found to harbor QTLs governing multiple traits. Eight chromosomes had more than four QTLs and were clustered together and designated as promising LTS tolerance QTLs (qLTTs), as qLTT1, qLTT2, qLTT3, qLTT5, qLTT6, qLTT8, qLTT9, and qLTT11. A total of 16 putative candidate genes were identified in the major M-QTLs and co-localized QTL regions distributed on different chromosomes. Overall, these significant genomic regions of M-QTLs are responsible for multiple traits and this suggested that these could serve as the best predictors of LTS tolerance at germination and early seedling growth stages. Furthermore, it is necessary to fine-map these regions and to find functional markers for marker-assisted selection in rice breeding programs for cold tolerance.

Ojiewo C.O., Janila P., Bhatnagar-Mathur P., Pandey M.K., Desmae H., Okori P., Mwololo J., Ajeigbe H., Njuguna-Mungai E., Muricho G., Akpo E., Gichohi-Wainaina W.N., Variath M.T., Radhakrishnan T., Dobariya K.L., et. al.

Groundnut is an important global food and oil crop, contributing to household food, nutrition and income security. Its consumption, processing and international trade is however, constrained by a number of genetic and biotic factors related to the grain nutrient profile. These include Aflatoxin, which is a prominent food safety challenge, allergens and oil profile, especially oleic/linoleic acid balance. This review paper highlights technological advances in crop improvement research to improve groundnut nutrient profile for better food, nutrition, health, and economic outcomes. Significant advances have been made in setting stage for marker-assisted allele pyramiding for different aflatoxin resistance mechanisms - in vitro seed colonization, pre-harvest aflatoxin contamination and aflatoxin production - which, together with pre- and post-harvest management practices, will go a long way in mitigating the Aflatoxin menace. A breakthrough in aflatoxin control is in sight with overexpression of antifungal plant defensins, and through host-induced gene silencing in the aflatoxin biosynthetic pathway. Similarly, genomic and biochemical approaches to allergen control are in good progress, with the identification of homologs of the allergen encoding genes and development of monoclonal antibody based ELISA protocol to screen for and quantify major allergens. Double mutation of the allotetraploid homeologous genes, FAD2A and FAD2B, has shown potential for achieving >75% oleic acid as demonstrated among introgression lines. Significant advances have been made in seed systems research to bridge the gap between trait discovery, deployment and delivery through innovative partnerships and action learning.

Kumar A., Raman A., Yadav S., Verulkar S.B., Mandal N.P., Singh O.N., Swain P., Ram T., Badri J., Dwivedi J.L., Das S.P., Singh S.K., Singh S.P., Kumar S., Jain A., et. al.

• Genetic gain for rice grain yield for International Rice Research Institute drought breeding program was estimated. • Positive trend of 0.68 %, 0.87 %, 1.9 % under irrigated control, moderate and severe drought achieved. • Superiority of new rice varieties over currently grown demonstrated on farmers’ fields. • International Rice Research Institute developed rice varieties can protect farmers from crop losses under drought conditions. The complexity of genotype × environment interactions under drought reduces heritability, which determines the effectiveness of selection for drought tolerance and development of drought tolerant varieties. Genetic progress measured through changes in yield performance over time is important in determining the efficiency of breeding programmes in which test cultivars are replaced each year on the assumption that the new cultivars will surpass the older cultivars. The goal of our study was to determine the annual rate of genetic gain for rice grain yield in a drought-prone rainfed system in a series of multi-environment trials conducted from 2005 to 2014 under the Drought Breeding Network of Indian sites in collaboration with the International Rice Research Institute (IRRI). Our results show a positive trend in grain yield with an annual genetic yield increase of about 0.68 % under irrigated control, 0.87 % under moderate reproductive stage drought stress and 1.9 % under severe reproductive stage drought stress due to breeding efforts. The study also demonstrates the effectiveness of direct selection for grain yield under both irrigated control as well as managed drought stress screening to improve yield in typical rainfed systems. IRRI's drought breeding programme has exhibited a significant positive trend in genetic gain for grain yield over the years under both drought stress as well as favorable irrigated control conditions. Several drought tolerant varieties released from the programme have outperformed the currently grown varieties under varied conditions in the rainfed environments on farmers’ fields.

Bera S.K., Kamdar J.H., Kasundra S.V., Patel S.V., Jasani M.D., Maurya A.K., Dash P., Chandrashekar A.B., Rani K., Manivannan N., Janila P., Pandey M.K., Vasanthi R.P., Dobariya K.L., Radhakrishnan T., et. al.

Peanut (Arachis hypogaea L.) is an important nutrient-rich food legume and valued for its good quality cooking oil. The fatty acid content is the major determinant of the quality of the edible oil. The oils containing higher monounsaturated fatty acid are preferred for improved shelf life and potential health benefits. Therefore, a high oleic/linoleic fatty acid ratio is the target trait in an advanced breeding program. The two mutant alleles, ahFAD2A (on linkage group a09) and ahFAD2B (on linkage group b09) control fatty acid composition for higher oleic/linoleic ratio in peanut. In the present study, marker-assisted backcrossing was employed for the introgression of two FAD2 mutant alleles from SunOleic95R into the chromosome of ICGV06100, a high oil content peanut breeding line. In the marker-assisted backcrossing-introgression lines, a 97% increase in oleic acid, and a 92% reduction in linoleic acid content was observed in comparison to the recurrent parent. Besides, the oleic/linoleic ratio was increased to 25 with respect to the recurrent parent, which was only 1.2. The most significant outcome was the stable expression of oil-content, oleic acid, linoleic acid, and palmitic acid in the marker-assisted backcrossing-introgression lines over the locations. No significant difference was observed between high oleic and normal oleic in peanuts for seedling traits except germination percentage. In addition, marker-assisted backcrossing-introgression lines exhibited higher yield and resistance to foliar fungal diseases, i.e., late leaf spot and rust.