Open Access

Antimicrobial Agents and Chemotherapy, volume 60, issue 12, pages AAC.02117-16

Sorting out antibiotics' mechanisms of action: A double fluorescent protein reporter for high-throughput screening of ribosome and DNA biosynthesis inhibitors

Osterman Ilya A. ^{1, 2}

Komarova Ekaterina S. ³

Shiryaev Dmitry I ¹

Korniltsev Ilya A ¹

Khven Irina M ³

Lukyanov Dmitry A ¹

Tashlitsky Vadim N ¹

Serebryakova Marina V. ¹

Efremenkova Olga V ⁴

Ivanenkov Yan A ⁵

Bogdanov Alexey A. ¹

Sergiev Petr V. ^{1, 2}

Dontsova Olga A. ^{1, 2}

Hide authors affiliations Show authors affiliations: 5 affiliations

Lomonosov Moscow State University, Department of Chemistry and A. N. Belozersky Institute of Physico-Chemical Biology, Moscow, Russia |

Skolkovo Institute of Science and Technology, Skolkovo, Russia |

Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics, Moscow, Russia |

⁴

G. F. Gauze Institute for Search for New Antibiotics, Russian Academy of Medical Sciences, Moscow, Russia |

⁵

Moscow Institute of Physics and Technology (State University), Moscow region, Russia |

Publication type: Journal Article

Publication date: 2016-12-01

American Society for Microbiology

Journal: Antimicrobial Agents and Chemotherapy

Quartile SCImago

Quartile WOS

Impact factor: 4.9

ISSN: 00664804, 10986596

DOI: 10.1128/AAC.02117-16

Copy DOI

PubMed ID: 27736765

Pharmacology

Infectious Diseases

Pharmacology (medical)

Abstract

ABSTRACT

In order to accelerate drug discovery, a simple, reliable, and cost-effective system for high-throughput identification of a potential antibiotic mechanism of action is required. To facilitate such screening of new antibiotics, we created a double-reporter system for not only antimicrobial activity detection but also simultaneous sorting of potential antimicrobials into those that cause ribosome stalling and those that induce the SOS response due to DNA damage. In this reporter system, the red fluorescent protein gene rfp was placed under the control of the SOS-inducible sulA promoter. The gene of the far-red fluorescent protein, katushka2S , was inserted downstream of the tryptophan attenuator in which two tryptophan codons were replaced by alanine codons, with simultaneous replacement of the complementary part of the attenuator to preserve the ability to form secondary structures that influence transcription termination. This genetically modified attenuator makes possible Katushka2S expression only upon exposure to ribosome-stalling compounds. The application of red and far-red fluorescent proteins provides a high signal-to-background ratio without any need of enzymatic substrates for detection of the reporter activity. This reporter was shown to be efficient in high-throughput screening of both synthetic and natural chemicals.

By date By citations

Citations by journals

	1 2 3 4 5 6
Antibiotics	Antibiotics, 6, 10.91% Antibiotics 6 publications, 10.91%
Frontiers in Microbiology	Frontiers in Microbiology, 4, 7.27% Frontiers in Microbiology 4 publications, 7.27%
Pharmaceuticals	Pharmaceuticals, 2, 3.64% Pharmaceuticals 2 publications, 3.64%
Microorganisms	Microorganisms, 2, 3.64% Microorganisms 2 publications, 3.64%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 2, 3.64% International Journal of Molecular Sciences 2 publications, 3.64%
Microbiology	Microbiology, 2, 3.64% Microbiology 2 publications, 3.64%
Nature Chemical Biology	Nature Chemical Biology, 1, 1.82% Nature Chemical Biology 1 publication, 1.82%
Molecular Diversity	Molecular Diversity, 1, 1.82% Molecular Diversity 1 publication, 1.82%
Anti-Infective Agents	Anti-Infective Agents, 1, 1.82% Anti-Infective Agents 1 publication, 1.82%
Journal of Antibiotics	Journal of Antibiotics, 1, 1.82% Journal of Antibiotics 1 publication, 1.82%
Frontiers in Pharmacology	Frontiers in Pharmacology, 1, 1.82% Frontiers in Pharmacology 1 publication, 1.82%
Combinatorial Chemistry and High Throughput Screening	Combinatorial Chemistry and High Throughput Screening, 1, 1.82% Combinatorial Chemistry and High Throughput Screening 1 publication, 1.82%
Molecular Imaging and Biology	Molecular Imaging and Biology, 1, 1.82% Molecular Imaging and Biology 1 publication, 1.82%
Molecules	Molecules, 1, 1.82% Molecules 1 publication, 1.82%
Journal of the American Chemical Society	Journal of the American Chemical Society, 1, 1.82% Journal of the American Chemical Society 1 publication, 1.82%
Biochimie	Biochimie, 1, 1.82% Biochimie 1 publication, 1.82%
Bioorganic and Medicinal Chemistry Letters	Bioorganic and Medicinal Chemistry Letters, 1, 1.82% Bioorganic and Medicinal Chemistry Letters 1 publication, 1.82%
Acta Naturae	Acta Naturae, 1, 1.82% Acta Naturae 1 publication, 1.82%
Mendeleev Communications	Mendeleev Communications, 1, 1.82% Mendeleev Communications 1 publication, 1.82%
Biochemistry (Moscow)	Biochemistry (Moscow), 1, 1.82% Biochemistry (Moscow) 1 publication, 1.82%
Natural Product Communications	Natural Product Communications, 1, 1.82% Natural Product Communications 1 publication, 1.82%
Nucleic Acids Research	Nucleic Acids Research, 1, 1.82% Nucleic Acids Research 1 publication, 1.82%
Marine Drugs	Marine Drugs, 1, 1.82% Marine Drugs 1 publication, 1.82%
Insects	Insects, 1, 1.82% Insects 1 publication, 1.82%
Life	Life, 1, 1.82% Life 1 publication, 1.82%
Angewandte Chemie - International Edition	Angewandte Chemie - International Edition, 1, 1.82% Angewandte Chemie - International Edition 1 publication, 1.82%
Angewandte Chemie	Angewandte Chemie, 1, 1.82% Angewandte Chemie 1 publication, 1.82%
Medicinal Research Reviews	Medicinal Research Reviews, 1, 1.82% Medicinal Research Reviews 1 publication, 1.82%
Eurasian Soil Science	Eurasian Soil Science, 1, 1.82% Eurasian Soil Science 1 publication, 1.82%
	1 2 3 4 5 6

Citations by publishers

	2 4 6 8 10 12 14 16 18
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 17, 30.91% Multidisciplinary Digital Publishing Institute (MDPI) 17 publications, 30.91%
Springer Nature	Springer Nature, 6, 10.91% Springer Nature 6 publications, 10.91%
Frontiers Media S.A.	Frontiers Media S.A., 5, 9.09% Frontiers Media S.A. 5 publications, 9.09%
Pleiades Publishing	Pleiades Publishing, 4, 7.27% Pleiades Publishing 4 publications, 7.27%
Elsevier	Elsevier, 3, 5.45% Elsevier 3 publications, 5.45%
Wiley	Wiley, 3, 5.45% Wiley 3 publications, 5.45%
Bentham Science	Bentham Science, 2, 3.64% Bentham Science 2 publications, 3.64%
American Chemical Society (ACS)	American Chemical Society (ACS), 2, 3.64% American Chemical Society (ACS) 2 publications, 3.64%
Acta Naturae Ltd	Acta Naturae Ltd, 1, 1.82% Acta Naturae Ltd 1 publication, 1.82%
SAGE	SAGE, 1, 1.82% SAGE 1 publication, 1.82%
Oxford University Press	Oxford University Press, 1, 1.82% Oxford University Press 1 publication, 1.82%
FARC of the North-East named N.V. Rudnitskogo	FARC of the North-East named N.V. Rudnitskogo, 1, 1.82% FARC of the North-East named N.V. Rudnitskogo 1 publication, 1.82%
American Association for the Advancement of Science (AAAS)	American Association for the Advancement of Science (AAAS), 1, 1.82% American Association for the Advancement of Science (AAAS) 1 publication, 1.82%
American Society for Microbiology	American Society for Microbiology, 1, 1.82% American Society for Microbiology 1 publication, 1.82%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 1, 1.82% Royal Society of Chemistry (RSC) 1 publication, 1.82%
Taylor & Francis	Taylor & Francis, 1, 1.82% Taylor & Francis 1 publication, 1.82%
Cold Spring Harbor Laboratory	Cold Spring Harbor Laboratory, 1, 1.82% Cold Spring Harbor Laboratory 1 publication, 1.82%
	2 4 6 8 10 12 14 16 18

We do not take into account publications that without a DOI.
Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
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GOST Copy

Osterman I. A. et al. Sorting out antibiotics' mechanisms of action: A double fluorescent protein reporter for high-throughput screening of ribosome and DNA biosynthesis inhibitors // Antimicrobial Agents and Chemotherapy. 2016. Vol. 60. No. 12. p. AAC.02117-16.

GOST all authors (up to 50) Copy

Osterman I. A., Komarova E. S., Shiryaev D. I., Korniltsev I. A., Khven I. M., Lukyanov D. A., Tashlitsky V. N., Serebryakova M., Efremenkova O. V., Ivanenkov Y. A., Bogdanov A. A., Sergiev P. V., Dontsova O. A. Sorting out antibiotics' mechanisms of action: A double fluorescent protein reporter for high-throughput screening of ribosome and DNA biosynthesis inhibitors // Antimicrobial Agents and Chemotherapy. 2016. Vol. 60. No. 12. p. AAC.02117-16.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1128/AAC.02117-16

UR - https://doi.org/10.1128%2FAAC.02117-16

TI - Sorting out antibiotics' mechanisms of action: A double fluorescent protein reporter for high-throughput screening of ribosome and DNA biosynthesis inhibitors

T2 - Antimicrobial Agents and Chemotherapy

AU - Osterman, Ilya A.

AU - Komarova, Ekaterina S.

AU - Shiryaev, Dmitry I

AU - Korniltsev, Ilya A

AU - Khven, Irina M

AU - Lukyanov, Dmitry A

AU - Serebryakova, Marina V.

AU - Sergiev, Petr V.

AU - Dontsova, Olga A.

AU - Tashlitsky, Vadim N

AU - Efremenkova, Olga V

AU - Ivanenkov, Yan A

AU - Bogdanov, Alexey A.

PY - 2016

DA - 2016/12/01 00:00:00

PB - American Society for Microbiology

SP - AAC.02117-16

IS - 12

VL - 60

PMID - 27736765

SN - 0066-4804

SN - 1098-6596

ER -

BibTex |

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BibTex Copy

@article{2016_Osterman,

author = {Ilya A. Osterman and Ekaterina S. Komarova and Dmitry I Shiryaev and Ilya A Korniltsev and Irina M Khven and Dmitry A Lukyanov and Marina V. Serebryakova and Petr V. Sergiev and Olga A. Dontsova and Vadim N Tashlitsky and Olga V Efremenkova and Yan A Ivanenkov and Alexey A. Bogdanov},

title = {Sorting out antibiotics' mechanisms of action: A double fluorescent protein reporter for high-throughput screening of ribosome and DNA biosynthesis inhibitors},

journal = {Antimicrobial Agents and Chemotherapy},

year = {2016},

volume = {60},

publisher = {American Society for Microbiology},

month = {dec},

url = {https://doi.org/10.1128%2FAAC.02117-16},

number = {12},

pages = {AAC.02117--16},

doi = {10.1128/AAC.02117-16}

}

MLA

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MLA Copy

Osterman, Ilya A., et al. “Sorting out antibiotics' mechanisms of action: A double fluorescent protein reporter for high-throughput screening of ribosome and DNA biosynthesis inhibitors.” Antimicrobial Agents and Chemotherapy, vol. 60, no. 12, Dec. 2016, pp. AAC.02117-16. https://doi.org/10.1128%2FAAC.02117-16.

Found error?

Publisher

American Society for Microbiology

Journal

Antimicrobial Agents and Chemotherapy

Quartile SCImago

Quartile WOS

Impact factor

4.9

ISSN

00664804 (Print)
10986596 (Electronic)

Labs

Laboratory of Nucleoprotein Chemistry, Department of Chemistry of Natural Compounds, Faculty of Chemistry

Profiles