Journal on Computing and Cultural Heritage
SCImago
Q1
Impact factor
2.1
SJR
0.632
CiteScore
4.6
Categories
Conservation
Computer Graphics and Computer-Aided Design
Computer Science Applications
Information Systems
Areas
Arts and Humanities
Computer Science
Years of issue
2008-2025
journal names
Journal on Computing and Cultural Heritage
ACM J COMPUT CULT HE
Top-3 citing journals
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Journal on Computing and Cultural Heritage
(498 citations)
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Lecture Notes in Computer Science
(363 citations)
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Applied Sciences (Switzerland)
(154 citations)
Top-3 organizations
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University College London
(10 publications)
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University of Bologna
(10 publications)
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King's College London
(8 publications)

Aegean University
(8 publications)
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University of Bologna
(7 publications)
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Ghent University
(6 publications)
Top-3 countries
Most cited in 5 years
Found
Publications found: 438
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The priority of bacteria Staphylococcus spp. in the etiology of PSI through the prism of new diagnostic capabilities. Literature review.
Borisov A., Golubkova A.A., Ruzhentsova T.A.
Representatives of the genus Staphylococcus spp. occupy a leading place in the etiology of infections caused by opportunistic bacteria. Staphylococcus spp., being symbionts of humans and/or animals, are constantly located on the surface of the skin and mucous membranes, which are their natural habitats.
The importance of bacteria of the genus Staphylococcus spp. It is determined by their significant distribution in the population, as well as among patients and medical personnel, due to their transient and resident carrier, as well as their high adaptive potential, due to their ability to form biofilms and produce enzymes that provide them with protection from antimicrobial drugs.
Modern molecular genetic methods for identifying Staphylococcus spp. and assessing their epidemic potential allow us to rethink their importance in the etiology of nosocomial purulent septic infections in a new way. It is no coincidence that they entered the group of ESCAPE pathogens with a high level of priority.
The ability of staphylococci to form biofilms ensures their survival in various hospital/environmental facilities, which in the conditions of a medical organization become additional, or so-called “inanimate” sources of infection.
In recent years, despite the increasing role of coagulase-negative staphylococci in the etiology of purulent septic infections, Staphylococcus aureus remains the most significant causative agent of purulent septic infections.
Q4
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Mechanisms of Antiphage Defense in Prokaryotes
Daudova А.D., Demina Y.Z., Abdrakhmanova R.O., Baeva G.R., Rubalskaia T.S., Yasenyavskaya A.L., Rubalsky O.V., Samotrueva M.A.
The globalization of the problem of the formation of bacterial strains poly- and pan-resistant to known antimicrobial drugs creates high risks in the healthcare sector. The threat of a return to the «pre-antibiotic» era dictates the need to search for alternative forms of antibacterial therapy. Phage therapy, based on the use of a natural, widely distributed in the environment, accessible antimicrobial agent, is again becoming relevant. The article highlights the mechanisms of antiviral protection of prokaryotes at various stages of interaction between the virus and the target cell. Revealing the secrets of the confrontation between bacteria and viruses, their co-evolution is necessary to increase the effectiveness of phage therapy and develop modern means of overcoming bacterial resistance to antimicrobial agents.
Q1
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A Novel Bifidobacterium longum Subsp. longum T1 Strain from Cow’s Milk: Homeostatic and Antibacterial Activity against ESBL-Producing Escherichia coli
Machulin A.V., Abramov V.M., Kosarev I.V., Deryusheva E.I., Priputnevich T.V., Panin A.N., Manoyan A.M., Chikileva I.O., Abashina T.N., Blumenkrants D.A., Ivanova O.E., Papazyan T.T., Nikonov I.N., Suzina N.E., Melnikov V.G., et. al.
Background/Objectives: The global emergence of antibiotic-resistant zooanthroponotic Escherichia coli strains, producing extended-spectrum beta-lactamases (ESBL-E) and persisting in the intestines of farm animals, has now led to the development of a pandemic of extra-intestinal infectious diseases in humans. The search for innovative probiotic microorganisms that eliminate ESBL-E from the intestines of humans and animals is relevant. Previously, we received three isolates of bifidobacteria: from milk of a calved cow (BLLT1), feces of a newborn calf (BLLT2) and feces of a three-year-old child who received fresh milk from this calved cow (BLLT3). Our goal was to evaluate the genetic identity of BLLT1, BLLT2, BLLT3 isolates using genomic DNA fingerprinting (GDF), to study the tolerance, adhesion, homeostatic and antibacterial activity of BLLT1 against ESBL-E. Methods: We used a complex of microbiological, molecular biological, and immunological methods, including next generation sequencing (NGS). Results: GDF showed that DNA fragments of BLLT2 and BLLT3 isolates were identical in number and size to DNA fragments of BLLT1. These data show for the first time the possibility of natural horizontal transmission of BLLT1 through with the milk of a calved cow into the intestines of a calf and the intestines of a child. BLLT1 was resistant to gastric and intestinal stresses and exhibited high adhesive activity to calf, pig, chicken, and human enterocytes. This indicates the unique ability of BLLT1 to inhabit the intestines of animals and humans. We are the first to show that BLLT1 has antibacterial activity against ESBL-E strains that persist in humans and animals. BLLT1 produced 145 ± 8 mM of acetic acid, which reduced the pH of the nutrient medium from 6.8 to 5.2. This had an antibacterial effect on ESBL-E. The genome of BLLT1 contains ABC-type carbohydrate transporter gene clusters responsible for the synthesis of acetic acid with its antibacterial activity against ESBL-E. BLLT1 inhibited TLR4 mRNA expression induced by ESBL-E in HT-29 enterocytes, and protected the enterocyte monolayers used in this study as a bio-model of the intestinal barrier. BLLT1 increased intestinal alkaline phosphatase (IAP) as one of the main molecular factors providing intestinal homeostasis. Conclusions: BLLT1 shows promise for the creation of innovative functional nutritional products for humans and feed additives for farm animals that will reduce the spread of ESBL-E strains in the food chain.
Q4
Analysis of the course and outcomes of COVID-19 at different stages of the pandemic in hemodialysis patients
Frolova N.F., Kim I.G., Chervinko V.I., Volodina E.V., Zubkin M.L.
The purpose of this study was a comparative analysis of the characteristics of the course and outcomes of COVID-19 in HD patients at different stages of the pandemic, focusing on the of the use of immunomodulatory therapy.Materials and methods. The retrospective study included 897 HD patients with COVID-19 (mean age 60.7 years, M 58.5%) who were hospitalized at Moscow City Hospital № 52. Group 1 (n=720) consisted of patients infected between the end of March 2020 and April 2021, group 2 (n=177) included patients hospitalized in May-December 2021. Each of group was divided into 2 subgroups based on treatment approaches. Subgroup 1a (n=231) included patients of the initial period of the pandemic who did not receive adequate immunomodulatory therapy, while Subgroup 1b (n=489) included patients of the late stage, were treated with IL-6 receptor blockers and corticosteroids. In group 2, 108 patients in Subgroup 2a received similar therapy, while 69 patients in Subgroup 2b were treated with neutralizing monoclonal antibodies in the early stages of the disease.Results. Mortality rates in Group 1 and Group 2 was 20.1% and 14.7%, respectively (p<0.09). The incidence of unfavorable outcome was highest in Subgroup 1a and lowest in Subgroup 2b (31.2% vs 5.8%, p<0.01). Mortality in Subgroups 1b and 2a was comparable (14.9% and 20.4%), despite more severe initial lung damage according to CT data in Subgroup 2a. In these patients, immunomodulators was more frequently combined with therapeutic plasma exchange (TPE). Independent risk factors for an unfavorable outcome were the progression of pulmonary pathology, with the transformation of stages CT 1-2 to CT 3-4, and a high comorbidity index.Conclusions. The use of immunomodulatory drugs imprtoved the effectiveness of COVID-19 treatment in patients with CKD5D. In severe cases, the most favorable outcomes were achieved with a combination of immunobiological drugs, corticosteroids, and TPE. An even more significant reduction in mortality was observed following the introduction of neutralizing monoclonal antibodies into clinical practice. Independent predictors of unfavorable outcome of COVID-19 in HD patients were a high comorbidity index and the progression of CT 1-2 into CT 3-4.
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Features of the course of COVID-19 in children with the spread of the Omicron variant
Ruzhentsova T.A., Garbuzov A.A., Bitsueva A.V., Rubleva O.V., Gorbunov S.G., Meshkova N.A., Popova R.V., Morozova E.N.
The aim of the study was to assess the symptoms and identify risk factors for prolonged and complicated COVID-19 during the spread of the Omicron variant in children aged 1 month to 18 years. Materials and methods. The study included 110 children with confirmed COVID-19 of mild or moderate severity at the time of the first examination. The average age of the patients was 6.86±5.09 years. The clinical symptoms, duration of each of the identified symptoms, and complications were evaluated. Acomparative analysis of possible risk factors for prolonged course and development of pneumonia was carried out. Results. The most common cases in children with COVID-19 were an increase in body temperature (in93%), runny nose (in65%), cough (in59%), hyperemia of the posterior pharyngeal wall (in95%) and tonsils (in93%), an increase in regional lymph nodes - in 43%. Among children with the duration of COVID-19 for 10 days or more, astatistically significant late request for medical help (onaverage, by 8.26±5.46 days, compared with 3.00 ± 1.32 days with the duration of the disease up to 10 days). Pneumonia is most often registered among preschool children. The average age in the group of children diagnosed with pneumonia is significantly less than in children who did not have signs of pneumonia of this complication (4.66 ±4.78 and 7.17±4.81, respectively). Conclusions: With the spread of the Omicron variant COVID-19 in children, infection with the SARS-CoV-2 coronavirus is accompanied by symptoms of acute respiratory infection with an increase in body temperature, sore throat, hyperemia of the posterior wall of the pharynx and tonsils. Often there is an increase in regional lymph nodes, runny nose, cough, vomiting, less often - diarrhea. The prolonged course is facilitated by the late onset of adequate therapy. During the study, pneumonia was detected in 25% of children.
Q1
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Subgroup Analysis of TLR2, -3, -4 and -8 in Relation to the Severity of Clinical Manifestations of Cervical HPV Infection
Dushkin A., Afanasiev M., Afanasiev S., Grishacheva T., Biryukova E., Dushkina I., Karaulov A.
Q1
International Journal of Molecular Sciences
,
2024
,
citations by CoLab: 0
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Open Access
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PDF
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Abstract
We present the findings of assessing the expression levels of extracellular TLR2 and TLR4 and intracellular TLR3 and TLR8 correlating with the severity of clinical manifestations of HPV infection. A total of 199 women took part in a single-center prospective comparative research study on TLR2, TLR3, TLR4 and TLR8 expression in HPV-related cervical lesions. TLRs’ mRNA expression was analyzed using real-time reverse transcription polymerase chain reaction (RT-PCR). Our results indicate the potential significance of TLR3, TLR4 and TLR8 in responding to HPV infection and its progression to SILs and CC, highlighting the importance of HPV polyinfection in relation to TLR4 and TLR8.
Q4
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Optimal choice of indicators for timely assessment of the prognosis of COVID-19 in hospitalized patients
Ruzhentsova T.A., Mirzajonova D.B., Zhanibekov Z.Z., Khavkina D.A., Chukhliaev P.V., Borisova O.Y., Meshkova N.A.
The aim of the study was to identify the indicators that are most significant for predicting the features of the course of COVID-19 in hospitalized patients.Materials and methods. 250 case histories of patients aged 18 to 86 years with COVID-19 hospitalized in the hospital of the city of Nukus, Republic of Uzbekistan, redesigned to provide care to patients with COVID-19 from July 1, 2020 to March 2022, were analyzed. Patients who had a wave–like course of the disease with the development of complications, an increase in the volume of lung damage, were included in the main group (62 patients, 3 of whom were extremely severe). The patients who had stable positive dynamics (188 people) formed a comparison group.Results and discussion. Among patients over 65 years of age, 36% had a complicated course, in the 45–65 age group — 29% (p>0.05), and among patients under 45 years of age — 13% (p<0.05). The main group was dominated by men (79%). Among hospitalized villagers, an increase in the severity of the condition was noted in 30% of cases, and among patients from the city of Nukus, such patients were 20% (p<0.05). The highest values of D-dimer, interleukin-6 and C-reactive protein were in both groups, significant differences between the groups were revealed in the levels of D-dimer and interleukin-6. Significant differences between the groups were found in the levels of D-dimer, interleukin-6, ferritin, ALT, AST, and urea.Conclusion. COVID-19 has become one of the most studied diseases to date, but aspects of the course of this disease in certain population groups are still not sufficiently investigated. The complicated, progressive course of COVID-19, according to the results of our study, was recorded in all age groups of the adult population, more often in men from rural areas aged over 45 years with chronic diseases. The main prognostic markers of the complicated and progressive course of COVID-19 should be considered high levels of D-dimer, interleukin-6 and ferritin.
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The Potential of Lectins and their Recognized Glycoconjugates in the Human Body
Lakhtin M.V., Lakhtin V.M., Mironov A.Y., Aleshkin V.A., Afanasyev S.S., Kombarova S.Y.
The modern concepts of lectins and glycoconjugates binding to them, the features and patterns of their interaction, the protective role and potential in the human body are summarized. The analysis of terms, approaches to classifications of lectins is carried out. The features of natural and synthetic glycoconjugates, recognized and bound by lectins, in symbiotic relationships, in innate immunity at the reception level are emphasized. The levels of specificity of lectins are considered. There is a need to expand research on the glycoconjugate specificity of lectins and their systems, to assess the communication potential of glycoconjugates in relation to any protein combinations and systems as lectins. The participation of lectin and glycoconjugate systems in signal transmission and communication is noted. Lectins manifest themselves as basic for superstructure glycoconjugate effectors in soluble and solid cell phases in cascade directed assemblies forming the interactome network. Lectins and glycoconjugates, as inextricably co-functioning, are promising in biology, medicine and biotechnologies.
Q3
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Isolation and Characterization of Salmonella Bacteriophages as Potential Agents for Phage Therapy of Antibiotic-Resistant Intestinal Infections
Pasivkina M.A., Anurova M.N., Kiseleva I.A., Andreeva A.A., Vorobev A.M., Mizaeva T.E., Mekhtiev E.R., Zubkova E.S., Alieva K.M., Kuzmin A.R., Karaulov A.V., Aleshkin A.V.
Two bacteriophages specifically active against to pathogenic strains of the Salmonella genus were isolated. The morphology of phage colonies (size, transparency, and shape of the plaque edge, and halo) and the spectrum of their lytic activity and interaction with microbial cells (adsorption rate, duration of the latency, and reproductive efficiency) were examined. Using genome-wide sequencing, we determined the taxonomic position of bacteriophages and verified the absence of unwanted genes encoding toxins, adhesins, and invasins, as well as pathogenicity islands responsible for antibiotic resistance. In addition, phage stability under different physical conditions and their productivity were studied.
Q3
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Assessment of the Safety of Anti-Salmonella Disinfectant for Veterinary Use Based on a Cocktail of Bacteriophages
Zulkarneev E.R., Laishevtsevtsev A.I., Kiseleva I.A., Efimova O.G., Mizaeva T.E., Pasivkina M.A., Zubkova E.S., Aleshkin A.V., Karaulov A.V.
The toxicity and safety of a veterinary anti-salmonella disinfectant based on three highly virulent bacteriophage strains (titers 1010 PFU/ml) were studied. Acute, chronic, and inhalation toxicity, as well as local irritancy of the disinfectant were evaluated on outbred white mice CD1 (n=65), Soviet chinchilla rabbits (n=20), and rats (n=20). No toxic effects of the disinfectant was observed after its intraperitoneal or intragastric administration to mice and intragastric administration to rats; in rabbits, application on the skin and eyes produced no local irritation effect. Inhalation of 10% of the disinfectant did not cause any pathologies in mice. Thus, the tests confirmed the high level of safety of the disinfectant based on a mixture of bacteriophages for use as an additional specific disinfection agent against Salmonella in veterinary and livestock facilities.
Q1
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Development of novel antimicrobials with engineered endolysin LysECD7-SMAP to combat Gram-negative bacterial infections
Vasina D.V., Antonova N.P., Gushchin V.A., Aleshkin A.V., Fursov M.V., Fursova A.D., Gancheva P.G., Grigoriev I.V., Grinkevich P., Kondratev A.V., Kostarnoy A.V., Lendel A.M., Makarov V.V., Nikiforova M.A., Pochtovyi A.A., et. al.
Abstract
Background
Among the non-traditional antibacterial agents in development, only a few targets critical Gram-negative bacteria such as carbapenem-resistant Pseudomonas aeruginosa, Acinetobacter baumannii or cephalosporin-resistant Enterobacteriaceae. Endolysins and their genetically modified versions meet the World Health Organization criteria for innovation, have a novel mode of antibacterial action, no known bacterial cross-resistance, and are being intensively studied for application against Gram-negative pathogens.
Methods
The study presents a multidisciplinary approach, including genetic engineering of LysECD7-SMAP and production of recombinant endolysin, its analysis by crystal structure solution following molecular dynamics simulations and evaluation of antibacterial properties. Two types of antimicrobial dosage forms were formulated, resulting in lyophilized powder for injection and hydroxyethylcellulose gel for topical administration. Their efficacy was estimated in the treatment of sepsis, and pneumonia models in BALB/c mice, diabetes-associated wound infection in the leptin receptor-deficient db/db mice and infected burn wounds in rats.
Results
In this work, we investigate the application strategies of the engineered endolysin LysECD7-SMAP and its dosage forms evaluated in preclinical studies. The catalytic domain of the enzyme shares the conserved structure of endopeptidases containing a putative antimicrobial peptide at the C-terminus of polypeptide chain. The activity of endolysins has been demonstrated against a range of pathogens, such as Klebsiella pneumoniae, A. baumannii, P. aeruginosa, Staphylococcus haemolyticus, Achromobacter spp, Burkholderia cepacia complex and Haemophylus influenzae, including those with multidrug resistance. The efficacy of candidate dosage forms has been confirmed in in vivo studies. Some aspects of the interaction of LysECD7-SMAP with cell wall molecular targets are also discussed.
Conclusions
Our studies demonstrate the potential of LysECD7-SMAP therapeutics for the systemic or topical treatment of infectious diseases caused by susceptible Gram-negative bacterial species and are critical to proceed LysECD7-SMAP-based antimicrobials trials to advanced stages.
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Forecasting changes in oral hygiene levels using the API index with different orthodontic appliances
Fedorova K.V., Gavrilova O.A., Zatevalov A.M.
Relevance. The prevalence of various types of orthodontic pathology among the Russian population is quite high. Depending on the region, malocclusion are registered in 30.9% to 76.5% of cases. The high prevalence of orthodontic pathology and the unresolved issues related to both the pathology itself and the presence of concomitant diseases indicate the necessity for personalized methods of prevention and treatment of malocclusion during both the primary and mixed dentitions, which in 70-84% of cases are treated using both removable and fixed orthodontic appliances.Materials and methods. An observational study was conducted to identify factors contributing to the increased intensity of caries among children aged 6 to 11 with orthodontic pathology. The study evaluated the characteristics of hygiene knowledge and skills, as well as the Approximal Plaque Index (API), in children using various orthodontic appliances. To assess hygiene knowledge levels, a survey was administered to two groups of children aged 6-11: those undergoing orthodontic treatment and those who visited the dental clinic for a routine check-up and were diagnosed with orthodontic pathology. The questionnaire included questions about the primary and supplementary tools and practices for daily oral hygiene, the frequency of hygiene activities, and the patients' awareness of the importance of professional oral hygiene.Results. Factors that statistically significantly influence the level of hygiene and the Approximal Plaque Index (API) were identified. A computer program was developed to forecast the dynamics of oral hygiene levels (based on the API index) when using different orthodontic appliances. An algorithm for predicting changes in the API hygiene index was created, considering the use of orthodontic appliances, oral status, adherence to hygiene procedures, presence of harmful habits, and dietary behaviors. Negative factors significantly impacting hygiene levels include bruxism and daily consumption of sweets, while positive factors include adherence to the recommended frequency and duration of tooth brushing (at least twice a day) and the use of a medium-hard toothbrush when permanent teeth are erupting. During orthodontic treatment, additional negative factors impacting hygiene status include gingival bleeding (gingivitis), lack of lip closure, and harmful habits. The study of changes in hygiene levels over one year of treatment with various orthodontic appliances (braces, Haas appliance, SPA) showed that the fixed Haas appliance for treating dental and maxillofacial anomalies is the most favorable orthodontic design for mixed dentition.Conclusion. Factors significantly affecting the level of oral hygiene have been identified. The dynamics of the API hygiene index with various orthodontic appliances have been established. The most favorable appliance for mixed dentition is the HAAS appliance for rapid palatal expansion.
Q4
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The State of Immunity of the Population of the Russian Federation to Rubella during the Elimination of Infection
Barkinkhoeva L.A., Turaeva N.V., Tsvirkun O.V., Gerasimova A.G.
Q4
Epidemiology and Vaccinal Prevention (Epidemiologiya i Vaktsinoprofilaktika)
,
2024
,
citations by CoLab: 0
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Open Access
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Abstract
Relevance. Serological monitoring of the state of the population's immunity to the rubella virus is one of the key elements of epidemiological surveillance of the infection, which makes it possible to assess the effectiveness of vaccine prevention, including the level of protection of the population from this infection. This article presents a comprehensive analysis of the results of this event in Russia for the period from 2015 to 2022 in individuals vaccinated against this infection, based on data from reports from regional centers and existing statistical models. The aim. To carry out a comprehensive analysis of data on serological monitoring of immunity among indicator groups of the population to rubella virus in the Russian Federation for the period from 2015 to 2022, taking into account the incidence and peculiarities of immunization tactics. Materials and Methods. Serological monitoring was carried out using standardized test systems and techniques, which ensured comparability of data throughout the entire study period (2015-2022). The contingent for the examination was selected with documentary confirmation of the fact of vaccination in the age groups 3–4 years, 9–10, 15–17, 25–29, 30–35 years. Only persons aged 40 years and older were examined without taking into account the vaccination history Results and Discussion. The conducted study confirmed a sufficient, stable level of immunity to the rubella virus in most age groups and regions of the country, which indicates the effectiveness of vaccination and the preservation (stabilization) of the infection elimination process. The slight increase in the average proportion of seronegatives among adults 30–35 and 40 years and older, revealed in the analysis, compared with other groups, requires further observation and clarification of the causes. Conclusions. The study confirmed the high level of immunity to rubella virus among the population of the Russian Federation, which indicates the effectiveness of the current program of vaccination against rubella infection and the infection elimination program. However, groups of the population have been identified, among which there is a slight decrease in the level of immunity, which requires additional attention from health authorities to maintain a stable rubella elimination status in the country. The results of the study will be used as additions to national and regional strategies aimed at maintaining the rubella elimination status.
Q4
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Antimicrobial Susceptibility Testing to Erythromycin and Azithromycin of Clinical Isolates of Bordetella pertussis Circulating in Russia
Pimenova A.S., Gadua N.T., Andrievskaya I.Y., Borisova O.Y., Petrova M.S., Borisova A.B., Afanas'ev S.S., Podoprigora I.V., Afanas'ev M.S., Moskvina T.I., Vorob'eva G.V., Degtyareva I.M., Timirkina O.V., Luk'yanceva S.A., Trigorlova T.N.
Q4
Epidemiology and Vaccinal Prevention (Epidemiologiya i Vaktsinoprofilaktika)
,
2024
,
citations by CoLab: 0
,
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Open Access
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Abstract
Relevance. Antibacterial drugs are widely used to treat and prevent infections of the upper and lower respiratory tract. The large-scale and unjustified use of antimicrobials to treat these infections has led to the emergence of resistance in most pathogens. The aim. To study antimicrobial susceptibility testing to erythromycin and azithromycin of strains of B. pertussis isolated in Russia. Materials & Methods. The research included 165 strains of B. pertussis isolated in January 2014 to June 2020. Antimicrobial susceptibility to erythromycin and azithromycin was determined by disk diffusion method and MIC test (HiMedia Laboratories Pvt. Limited, India). The A2047G mutation in the 23S rRNA gene was detected by PCR and subsequent sequencing. Results. Disk diffusion zone diameters for erythromycin in the studied strains ranged from 25 to 62 mm (median 44 mm) and disk diffusion zone diameters for azithromycin ranged from 22 to 80 mm (median 50 mm). Isolates with growth inhibition of more than 42 mm in diameter after 7 days of incubation were considered as susceptible. Among the studied strains, 57 (34.5%) were resistant to erythromycin and 23 (13.9%) to azithromycin. Then, MIC of erythromycin and MIC of azithromycin, respectively, were determined for these 57 and 23 strains using the MIC test. The comparison group included 79 isolates that were classified as sensitive to erythromycin (n = 31) and azithromycin (n=48) according to the results of the previous study. A MIC value of 0.12 μg/ml was considered as the cut-off for susceptible strains. All isolates were fully susceptible to erythromycin (MIC ≤ 0.01 μg/ml, median MIC 0.001 μg/ml) and azithromycin (MIC ≤ 0.01 μg/ml, median MIC 0.0001 μg/ml). An A-to-G mutation was not found at position 2047 in the 23S rRNA gene in 80 isolates that had a diameter of growth inhibition zone less than 42 mm. Conclusion. This study demonstrates no significant decrease in the susceptibility to erythromycin and azithromycin among B. pertussis strains isolated in Russia in 2014–2020. The studied B. pertussis strains exhibit a homozygous phenotype for macrolide resistance.
Q3
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Digital approach to estimate clinical images of the cervix with ImageJ software
Dushkin A.D., Afanasiev M.S., Afanasiev S.S., Grishacheva T.G., Karaulov A.V.
BACKGROUND: Visual inspection and colposcopy are subjective methods of cervical evaluation. Currently, the majority of colposcopes are equipped with the capacity to digitally transmit and record cervical images, in addition to modern software for image processing. For the objective assessment, prevention of development, and risk assessment of precancerous changes (SIL+) and cervical cancer, it is essential to use modern methods of image processing.
AIM: The study aimed at demonstrating the capabilities of digital analysis of cervical images based on ImageJ software [1].
MATERIALS AND METHODS: A total of 500 colposcopic images of the Schiller test were obtained during dilated colposcopy. Digital analysis was performed using ImageJ software, which employed minimum (MinGV) and maximum (MaxGV) gray pixel values (0–255) and lesion surface area (%Area) as parameters. The images were divided into 4 groups according to the cytologic examination performed: healthy donors (n=19; 3.8%), mild grade squamous cell intraepithelial lesion (n=113; 22.6%), severe grade squamous cell intraepithelial lesion (n=327; 65.4%), and invasive cervical cancer (n=41; 8.2%). Mathematical and statistical analysis of the obtained data was performed using Python programming language packages in the Google Colab environment. Comparisons of quantitative measures between three or more groups were conducted using the Kruskal-Wallis criterion and posteriori comparisons by Dunn’s criterion with Holm’s correction.
RESULSTS: Statistical significance was observed in the increase of MinGV (p=0.035), MaxGV (p0.001) and %Area (p=0.022) from the mild (88/141/31) to the severe (83/142/32) degree of squamous cell intraepithelial lesion and cervical cancer (88/162/36). Objective parameters for the assessment of the degree of cervical surface lesions during digital colposcopy were obtained. Digital analysis of the cervical surface may assist the clinical specialist in determining further management strategies, including scarification or incisional biopsy with subsequent morphological examination.
CONCLUSIONS: The application of digital analysis to colposcopic images has the potential to reduce the subjective assessment of cervical condition, enhance the efficiency of the initial appointment with a gynecologist, and facilitate the selection of patients for cytologic examination.
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Humboldt University of Berlin
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Tenaga National University
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University of Helsinki
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University of Lausanne
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Publishing organizations in 5 years
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2
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4
5
6
7
8
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Aegean University
8 publications, 2.22%
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University of Bologna
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Ghent University
6 publications, 1.67%
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University of Turin
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King's College London
5 publications, 1.39%
|
|
University of Pisa
5 publications, 1.39%
|
|
Utrecht University
5 publications, 1.39%
|
|
École Polytechnique Fédérale de Lausanne
4 publications, 1.11%
|
|
Aalto University
4 publications, 1.11%
|
|
Polytechnic University of Milan
4 publications, 1.11%
|
|
University College London
4 publications, 1.11%
|
|
Interuniversity Microelectronics Centre
4 publications, 1.11%
|
|
Marche Polytechnic University
4 publications, 1.11%
|
|
Trinity College Dublin
4 publications, 1.11%
|
|
Leiden University
4 publications, 1.11%
|
|
University of Porto
4 publications, 1.11%
|
|
Bournemouth University
4 publications, 1.11%
|
|
Zhejiang University
3 publications, 0.83%
|
|
University of Haifa
3 publications, 0.83%
|
|
Nanjing University
3 publications, 0.83%
|
|
Eindhoven University of Technology
3 publications, 0.83%
|
|
IT University of Copenhagen
3 publications, 0.83%
|
|
University of Padua
3 publications, 0.83%
|
|
University of Verona
3 publications, 0.83%
|
|
University of Nottingham
3 publications, 0.83%
|
|
Loughborough University
3 publications, 0.83%
|
|
University of Florence
3 publications, 0.83%
|
|
University of Bari Aldo Moro
3 publications, 0.83%
|
|
Sungkyunkwan University
3 publications, 0.83%
|
|
University of Texas at Austin
3 publications, 0.83%
|
|
University of Luxembourg
3 publications, 0.83%
|
|
University of Peloponnese
3 publications, 0.83%
|
|
Bibliotheca Hertziana – Max Planck Institute for Art History
3 publications, 0.83%
|
|
University of Amsterdam
3 publications, 0.83%
|
|
University of Maryland, College Park
3 publications, 0.83%
|
|
Universidade Estadual de Campinas
3 publications, 0.83%
|
|
Universidad Complutense de Madrid
3 publications, 0.83%
|
|
University of Girona
3 publications, 0.83%
|
|
Mersin University
2 publications, 0.56%
|
|
Bar-Ilan University
2 publications, 0.56%
|
|
Uppsala University
2 publications, 0.56%
|
|
University of Malaysia, Sarawak
2 publications, 0.56%
|
|
Nanjing Agricultural University
2 publications, 0.56%
|
|
University of Helsinki
2 publications, 0.56%
|
|
Wuhan University
2 publications, 0.56%
|
|
University of Lausanne
2 publications, 0.56%
|
|
University of Milan
2 publications, 0.56%
|
|
Norwegian University of Science and Technology
2 publications, 0.56%
|
|
University of Tsukuba
2 publications, 0.56%
|
|
University of L'Aquila
2 publications, 0.56%
|
|
University of Udine
2 publications, 0.56%
|
|
Institute of Information Science and Technologies
2 publications, 0.56%
|
|
Free University of Bozen-Bolzano
2 publications, 0.56%
|
|
Scuola Normale Superiore
2 publications, 0.56%
|
|
Institute of Cognitive Sciences and Technologies
2 publications, 0.56%
|
|
Korea Advanced Institute of Science and Technology
2 publications, 0.56%
|
|
Education University of Hong Kong
2 publications, 0.56%
|
|
University of California, Los Angeles
2 publications, 0.56%
|
|
Zhejiang Sci-Tech University
2 publications, 0.56%
|
|
University of Nottingham Ningbo China
2 publications, 0.56%
|
|
National and Kapodistrian University of Athens
2 publications, 0.56%
|
|
Lanzhou University
2 publications, 0.56%
|
|
University of Patras
2 publications, 0.56%
|
|
University of West Attica
2 publications, 0.56%
|
|
Ionian University
2 publications, 0.56%
|
|
Vrije Universiteit Amsterdam
2 publications, 0.56%
|
|
Ludwig Maximilian University of Munich
2 publications, 0.56%
|
|
Technical University of Darmstadt
2 publications, 0.56%
|
|
Rhenish Friedrich Wilhelm University of Bonn
2 publications, 0.56%
|
|
University of Erlangen–Nuremberg
2 publications, 0.56%
|
|
Brown University
2 publications, 0.56%
|
|
Austrian Centre for Digital Humanities and Cultural Heritage of the Austrian Academy of Sciences
2 publications, 0.56%
|
|
Universidad Politécnica de Madrid
2 publications, 0.56%
|
|
Sheffield Hallam University
2 publications, 0.56%
|
|
Polytechnic University of Catalonia
2 publications, 0.56%
|
|
University of Sussex
2 publications, 0.56%
|
|
University of Jaén
2 publications, 0.56%
|
|
Polytechnique Montréal
2 publications, 0.56%
|
|
University of York
2 publications, 0.56%
|
|
Russian State University named after A.N. Kosygin (Technology. Design. Art)
1 publication, 0.28%
|
|
Istanbul Technical University
1 publication, 0.28%
|
|
Tarbiat Modares University
1 publication, 0.28%
|
|
Istanbul University
1 publication, 0.28%
|
|
Information Technology University
1 publication, 0.28%
|
|
Imam Abdulrahman Bin Faisal University
1 publication, 0.28%
|
|
Ozyegin University
1 publication, 0.28%
|
|
Anna University
1 publication, 0.28%
|
|
Bursa Uludağ University
1 publication, 0.28%
|
|
Artvin Coruh University
1 publication, 0.28%
|
|
Istanbul Medeniyet University
1 publication, 0.28%
|
|
Gebze Technical University
1 publication, 0.28%
|
|
Istanbul Bilgi University
1 publication, 0.28%
|
|
University of Isfahan
1 publication, 0.28%
|
|
Kocaeli University
1 publication, 0.28%
|
|
Kadir Has University
1 publication, 0.28%
|
|
Gaziosmanpasa University
1 publication, 0.28%
|
|
Afyon Kocatepe University
1 publication, 0.28%
|
|
Ton Duc Thang University
1 publication, 0.28%
|
|
Ho Chi Minh City Open University
1 publication, 0.28%
|
|
Vietnam National University, Hanoi
1 publication, 0.28%
|
|
Show all (70 more) | |
1
2
3
4
5
6
7
8
|
Publishing countries
20
40
60
80
100
120
|
|
Italy
|
Italy, 116, 19.53%
Italy
116 publications, 19.53%
|
United Kingdom
|
United Kingdom, 79, 13.3%
United Kingdom
79 publications, 13.3%
|
USA
|
USA, 55, 9.26%
USA
55 publications, 9.26%
|
China
|
China, 51, 8.59%
China
51 publications, 8.59%
|
Germany
|
Germany, 40, 6.73%
Germany
40 publications, 6.73%
|
France
|
France, 37, 6.23%
France
37 publications, 6.23%
|
Spain
|
Spain, 37, 6.23%
Spain
37 publications, 6.23%
|
Greece
|
Greece, 36, 6.06%
Greece
36 publications, 6.06%
|
Netherlands
|
Netherlands, 26, 4.38%
Netherlands
26 publications, 4.38%
|
Switzerland
|
Switzerland, 17, 2.86%
Switzerland
17 publications, 2.86%
|
Austria
|
Austria, 16, 2.69%
Austria
16 publications, 2.69%
|
Israel
|
Israel, 14, 2.36%
Israel
14 publications, 2.36%
|
Turkey
|
Turkey, 13, 2.19%
Turkey
13 publications, 2.19%
|
Australia
|
Australia, 11, 1.85%
Australia
11 publications, 1.85%
|
Republic of Korea
|
Republic of Korea, 11, 1.85%
Republic of Korea
11 publications, 1.85%
|
Belgium
|
Belgium, 10, 1.68%
Belgium
10 publications, 1.68%
|
Canada
|
Canada, 9, 1.52%
Canada
9 publications, 1.52%
|
Finland
|
Finland, 9, 1.52%
Finland
9 publications, 1.52%
|
Portugal
|
Portugal, 8, 1.35%
Portugal
8 publications, 1.35%
|
India
|
India, 8, 1.35%
India
8 publications, 1.35%
|
Cyprus
|
Cyprus, 8, 1.35%
Cyprus
8 publications, 1.35%
|
Denmark
|
Denmark, 7, 1.18%
Denmark
7 publications, 1.18%
|
Ireland
|
Ireland, 7, 1.18%
Ireland
7 publications, 1.18%
|
Brazil
|
Brazil, 5, 0.84%
Brazil
5 publications, 0.84%
|
Sweden
|
Sweden, 5, 0.84%
Sweden
5 publications, 0.84%
|
South Africa
|
South Africa, 5, 0.84%
South Africa
5 publications, 0.84%
|
Japan
|
Japan, 5, 0.84%
Japan
5 publications, 0.84%
|
Iraq
|
Iraq, 4, 0.67%
Iraq
4 publications, 0.67%
|
Luxembourg
|
Luxembourg, 4, 0.67%
Luxembourg
4 publications, 0.67%
|
Malaysia
|
Malaysia, 4, 0.67%
Malaysia
4 publications, 0.67%
|
Morocco
|
Morocco, 4, 0.67%
Morocco
4 publications, 0.67%
|
Norway
|
Norway, 4, 0.67%
Norway
4 publications, 0.67%
|
Poland
|
Poland, 4, 0.67%
Poland
4 publications, 0.67%
|
Ukraine
|
Ukraine, 3, 0.51%
Ukraine
3 publications, 0.51%
|
Egypt
|
Egypt, 3, 0.51%
Egypt
3 publications, 0.51%
|
Indonesia
|
Indonesia, 3, 0.51%
Indonesia
3 publications, 0.51%
|
Iran
|
Iran, 3, 0.51%
Iran
3 publications, 0.51%
|
Malta
|
Malta, 3, 0.51%
Malta
3 publications, 0.51%
|
Mexico
|
Mexico, 3, 0.51%
Mexico
3 publications, 0.51%
|
Algeria
|
Algeria, 2, 0.34%
Algeria
2 publications, 0.34%
|
Vietnam
|
Vietnam, 2, 0.34%
Vietnam
2 publications, 0.34%
|
Jordan
|
Jordan, 2, 0.34%
Jordan
2 publications, 0.34%
|
Romania
|
Romania, 2, 0.34%
Romania
2 publications, 0.34%
|
Saudi Arabia
|
Saudi Arabia, 2, 0.34%
Saudi Arabia
2 publications, 0.34%
|
Serbia
|
Serbia, 2, 0.34%
Serbia
2 publications, 0.34%
|
Singapore
|
Singapore, 2, 0.34%
Singapore
2 publications, 0.34%
|
Thailand
|
Thailand, 2, 0.34%
Thailand
2 publications, 0.34%
|
Tunisia
|
Tunisia, 2, 0.34%
Tunisia
2 publications, 0.34%
|
Czech Republic
|
Czech Republic, 2, 0.34%
Czech Republic
2 publications, 0.34%
|
Russia
|
Russia, 1, 0.17%
Russia
1 publication, 0.17%
|
Bulgaria
|
Bulgaria, 1, 0.17%
Bulgaria
1 publication, 0.17%
|
Bosnia and Herzegovina
|
Bosnia and Herzegovina, 1, 0.17%
Bosnia and Herzegovina
1 publication, 0.17%
|
Qatar
|
Qatar, 1, 0.17%
Qatar
1 publication, 0.17%
|
Colombia
|
Colombia, 1, 0.17%
Colombia
1 publication, 0.17%
|
New Zealand
|
New Zealand, 1, 0.17%
New Zealand
1 publication, 0.17%
|
UAE
|
UAE, 1, 0.17%
UAE
1 publication, 0.17%
|
Pakistan
|
Pakistan, 1, 0.17%
Pakistan
1 publication, 0.17%
|
Palestine
|
Palestine, 1, 0.17%
Palestine
1 publication, 0.17%
|
San Marino
|
San Marino, 1, 0.17%
San Marino
1 publication, 0.17%
|
Slovenia
|
Slovenia, 1, 0.17%
Slovenia
1 publication, 0.17%
|
Somalia
|
Somalia, 1, 0.17%
Somalia
1 publication, 0.17%
|
Philippines
|
Philippines, 1, 0.17%
Philippines
1 publication, 0.17%
|
Croatia
|
Croatia, 1, 0.17%
Croatia
1 publication, 0.17%
|
Chile
|
Chile, 1, 0.17%
Chile
1 publication, 0.17%
|
Kosovo
|
Kosovo, 1, 0.17%
Kosovo
1 publication, 0.17%
|
Show all (35 more) | |
20
40
60
80
100
120
|
Publishing countries in 5 years
10
20
30
40
50
60
|
|
Italy
|
Italy, 60, 16.67%
Italy
60 publications, 16.67%
|
United Kingdom
|
United Kingdom, 45, 12.5%
United Kingdom
45 publications, 12.5%
|
China
|
China, 38, 10.56%
China
38 publications, 10.56%
|
Spain
|
Spain, 29, 8.06%
Spain
29 publications, 8.06%
|
USA
|
USA, 28, 7.78%
USA
28 publications, 7.78%
|
Germany
|
Germany, 23, 6.39%
Germany
23 publications, 6.39%
|
Greece
|
Greece, 22, 6.11%
Greece
22 publications, 6.11%
|
Netherlands
|
Netherlands, 19, 5.28%
Netherlands
19 publications, 5.28%
|
France
|
France, 14, 3.89%
France
14 publications, 3.89%
|
Turkey
|
Turkey, 11, 3.06%
Turkey
11 publications, 3.06%
|
Israel
|
Israel, 9, 2.5%
Israel
9 publications, 2.5%
|
Australia
|
Australia, 7, 1.94%
Australia
7 publications, 1.94%
|
Belgium
|
Belgium, 7, 1.94%
Belgium
7 publications, 1.94%
|
Republic of Korea
|
Republic of Korea, 7, 1.94%
Republic of Korea
7 publications, 1.94%
|
Switzerland
|
Switzerland, 7, 1.94%
Switzerland
7 publications, 1.94%
|
Portugal
|
Portugal, 6, 1.67%
Portugal
6 publications, 1.67%
|
Ireland
|
Ireland, 6, 1.67%
Ireland
6 publications, 1.67%
|
Finland
|
Finland, 6, 1.67%
Finland
6 publications, 1.67%
|
Austria
|
Austria, 5, 1.39%
Austria
5 publications, 1.39%
|
Denmark
|
Denmark, 5, 1.39%
Denmark
5 publications, 1.39%
|
India
|
India, 5, 1.39%
India
5 publications, 1.39%
|
Cyprus
|
Cyprus, 5, 1.39%
Cyprus
5 publications, 1.39%
|
Brazil
|
Brazil, 4, 1.11%
Brazil
4 publications, 1.11%
|
Sweden
|
Sweden, 4, 1.11%
Sweden
4 publications, 1.11%
|
Japan
|
Japan, 4, 1.11%
Japan
4 publications, 1.11%
|
Ukraine
|
Ukraine, 3, 0.83%
Ukraine
3 publications, 0.83%
|
Indonesia
|
Indonesia, 3, 0.83%
Indonesia
3 publications, 0.83%
|
Iran
|
Iran, 3, 0.83%
Iran
3 publications, 0.83%
|
Canada
|
Canada, 3, 0.83%
Canada
3 publications, 0.83%
|
Luxembourg
|
Luxembourg, 3, 0.83%
Luxembourg
3 publications, 0.83%
|
Malta
|
Malta, 3, 0.83%
Malta
3 publications, 0.83%
|
Poland
|
Poland, 3, 0.83%
Poland
3 publications, 0.83%
|
Algeria
|
Algeria, 2, 0.56%
Algeria
2 publications, 0.56%
|
Vietnam
|
Vietnam, 2, 0.56%
Vietnam
2 publications, 0.56%
|
Egypt
|
Egypt, 2, 0.56%
Egypt
2 publications, 0.56%
|
Jordan
|
Jordan, 2, 0.56%
Jordan
2 publications, 0.56%
|
Malaysia
|
Malaysia, 2, 0.56%
Malaysia
2 publications, 0.56%
|
Morocco
|
Morocco, 2, 0.56%
Morocco
2 publications, 0.56%
|
Norway
|
Norway, 2, 0.56%
Norway
2 publications, 0.56%
|
Romania
|
Romania, 2, 0.56%
Romania
2 publications, 0.56%
|
Thailand
|
Thailand, 2, 0.56%
Thailand
2 publications, 0.56%
|
South Africa
|
South Africa, 2, 0.56%
South Africa
2 publications, 0.56%
|
Russia
|
Russia, 1, 0.28%
Russia
1 publication, 0.28%
|
Bulgaria
|
Bulgaria, 1, 0.28%
Bulgaria
1 publication, 0.28%
|
Bosnia and Herzegovina
|
Bosnia and Herzegovina, 1, 0.28%
Bosnia and Herzegovina
1 publication, 0.28%
|
Iraq
|
Iraq, 1, 0.28%
Iraq
1 publication, 0.28%
|
Qatar
|
Qatar, 1, 0.28%
Qatar
1 publication, 0.28%
|
New Zealand
|
New Zealand, 1, 0.28%
New Zealand
1 publication, 0.28%
|
Pakistan
|
Pakistan, 1, 0.28%
Pakistan
1 publication, 0.28%
|
Palestine
|
Palestine, 1, 0.28%
Palestine
1 publication, 0.28%
|
San Marino
|
San Marino, 1, 0.28%
San Marino
1 publication, 0.28%
|
Saudi Arabia
|
Saudi Arabia, 1, 0.28%
Saudi Arabia
1 publication, 0.28%
|
Serbia
|
Serbia, 1, 0.28%
Serbia
1 publication, 0.28%
|
Slovenia
|
Slovenia, 1, 0.28%
Slovenia
1 publication, 0.28%
|
Somalia
|
Somalia, 1, 0.28%
Somalia
1 publication, 0.28%
|
Tunisia
|
Tunisia, 1, 0.28%
Tunisia
1 publication, 0.28%
|
Philippines
|
Philippines, 1, 0.28%
Philippines
1 publication, 0.28%
|
Chile
|
Chile, 1, 0.28%
Chile
1 publication, 0.28%
|
Kosovo
|
Kosovo, 1, 0.28%
Kosovo
1 publication, 0.28%
|
Show all (29 more) | |
10
20
30
40
50
60
|
1 profile journal article
Hartmann Tilo
49 publications,
2 024 citations
h-index: 21