Journal of Agricultural and Food Chemistry

, том 66 , издание 42 , страницы 11180-11187

New Method for Accurate Determination of Polyphenol Oxidase Activity Based on Reduction in SERS Intensity of Catechol

Тип публикации: Journal Article

Дата публикации: 2018-09-13

American Chemical Society (ACS)

Journal of Agricultural and Food Chemistry

scimago Q1

wos Q1

БС1

SJR: 1.215

CiteScore: 9.3

Impact factor: 6.2

ISSN: 00218561, 15205118

DOI: 10.1021/acs.jafc.8b03985

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PubMed ID: 30209938

General Chemistry

General Agricultural and Biological Sciences

Краткое описание

Rapid and accurate measurement of polyphenol oxidase (PPO) activity is important in the food industry as PPOs play a vital role in catalyzing enzymatic reactions. The aim of this study was to develop surface-enhanced Raman scattering (SERS) approach for accurate determination of PPO activity in fruit and vegetables using the reduction in SERS intensity of catechol in reaction medium. Within a certain catechol concentration, when a purified PPO solution was analyzed, the reduction in SERS intensity (Δ I) was linear to PPO activity ( Ec) in a wide range of 500-50 000 U/L, and a linear regression equation of log Δ I/Δ t = 0.6223 log Ec + 0.8072, with a correlation coefficient of 0.9689 and a limit of detection of 224.65 U/L, was obtained. The method was used for detecting PPO activity in apple and potato samples, and the results were compared with those obtained from colorimetric assay, which demonstrated that the proposed method could be successfully used for detecting PPO activity in food samples.

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Pan T. T. et al. New Method for Accurate Determination of Polyphenol Oxidase Activity Based on Reduction in SERS Intensity of Catechol // Journal of Agricultural and Food Chemistry. 2018. Vol. 66. No. 42. pp. 11180-11187.

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Pan T. T., Sun D., Paliwal J., Pu H., Wei Q. New Method for Accurate Determination of Polyphenol Oxidase Activity Based on Reduction in SERS Intensity of Catechol // Journal of Agricultural and Food Chemistry. 2018. Vol. 66. No. 42. pp. 11180-11187.

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TY - JOUR

DO - 10.1021/acs.jafc.8b03985

UR - https://doi.org/10.1021/acs.jafc.8b03985

TI - New Method for Accurate Determination of Polyphenol Oxidase Activity Based on Reduction in SERS Intensity of Catechol

T2 - Journal of Agricultural and Food Chemistry

AU - Pan, Ting Tiao

AU - Sun, Da-Wen

AU - Paliwal, J

AU - Pu, Hongbin

AU - Wei, Qingyi

PY - 2018

DA - 2018/09/13

PB - American Chemical Society (ACS)

SP - 11180-11187

IS - 42

VL - 66

PMID - 30209938

SN - 0021-8561

SN - 1520-5118

ER -

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@article{2018_Pan,

author = {Ting Tiao Pan and Da-Wen Sun and J Paliwal and Hongbin Pu and Qingyi Wei},

title = {New Method for Accurate Determination of Polyphenol Oxidase Activity Based on Reduction in SERS Intensity of Catechol},

journal = {Journal of Agricultural and Food Chemistry},

year = {2018},

volume = {66},

publisher = {American Chemical Society (ACS)},

month = {sep},

url = {https://doi.org/10.1021/acs.jafc.8b03985},

number = {42},

pages = {11180--11187},

doi = {10.1021/acs.jafc.8b03985}

}

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Pan, Ting Tiao, et al. “New Method for Accurate Determination of Polyphenol Oxidase Activity Based on Reduction in SERS Intensity of Catechol.” Journal of Agricultural and Food Chemistry, vol. 66, no. 42, Sep. 2018, pp. 11180-11187. https://doi.org/10.1021/acs.jafc.8b03985.

Издатель

American Chemical Society (ACS)

Журнал

Journal of Agricultural and Food Chemistry

scimago Q1

wos Q1

БС1

SJR

1.215

CiteScore

9.3

Impact factor

6.2

ISSN

00218561 (Print)

15205118 (Electronic)