Open Access
Molecules, volume 26, issue 22, pages 6804
Rapid Full-Cycle Technique to Control Adulteration of Meat Products: Integration of Accelerated Sample Preparation, Recombinase Polymerase Amplification, and Test-Strip Detection
Publication type: Journal Article
Publication date: 2021-11-11
PubMed ID:
34833896
Organic Chemistry
Drug Discovery
Physical and Theoretical Chemistry
Pharmaceutical Science
Molecular Medicine
Analytical Chemistry
Chemistry (miscellaneous)
Abstract
Verifying the authenticity of food products is essential due to the recent increase in counterfeit meat-containing food products. The existing methods of detection have a number of disadvantages. Therefore, simple, cheap, and sensitive methods for detecting various types of meat are required. In this study, we propose a rapid full-cycle technique to control the chicken or pig adulteration of meat products, including 3 min of crude DNA extraction, 20 min of recombinase polymerase amplification (RPA) at 39 °C, and 10 min of lateral flow assay (LFA) detection. The cytochrome B gene was used in the developed RPA-based test for chicken and pig identification. The selected primers provided specific RPA without DNA nuclease and an additional oligonucleotide probe. As a result, RPA–LFA, based on designed fluorescein- and biotin-labeled primers, detected up to 0.2 pg total DNA per μL, which provided up to 0.001% w/w identification of the target meat component in the composite meat. The RPA–LFA of the chicken and pig meat identification was successfully applied to processed meat products and to meat after heating. The results were confirmed by real-time PCR. Ultimately, the developed analysis is specific and enables the detection of pork and chicken impurities with high accuracy in raw and processed meat mixtures. The proposed rapid full-cycle technique could be adopted for the authentication of other meat products.
Citations by journals
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1
2
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Journal of Food Composition and Analysis
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Journal of Food Composition and Analysis
2 publications, 22.22%
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Theory and practice of meat processing
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Theory and practice of meat processing
2 publications, 22.22%
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Food Control
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Food Control
1 publication, 11.11%
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Food Chemistry Molecular Sciences
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Food Chemistry Molecular Sciences
1 publication, 11.11%
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Journal of Agricultural and Food Chemistry
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Journal of Agricultural and Food Chemistry
1 publication, 11.11%
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Advanced NanoBiomed Research
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Advanced NanoBiomed Research
1 publication, 11.11%
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1
2
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Citations by publishers
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1
2
3
4
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Elsevier
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Elsevier
4 publications, 44.44%
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V. M. Gorbatov Federal Research Center for Food Systems RAS
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V. M. Gorbatov Federal Research Center for Food Systems RAS
2 publications, 22.22%
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American Chemical Society (ACS)
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American Chemical Society (ACS)
1 publication, 11.11%
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Wiley
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Wiley
1 publication, 11.11%
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1
2
3
4
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Ivanov A. V. et al. Rapid Full-Cycle Technique to Control Adulteration of Meat Products: Integration of Accelerated Sample Preparation, Recombinase Polymerase Amplification, and Test-Strip Detection // Molecules. 2021. Vol. 26. No. 22. p. 6804.
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Ivanov A. V., Popravko D. S., Safenkova I., Zvereva E. A., Dzantiev B. B., Zherdev A. V. Rapid Full-Cycle Technique to Control Adulteration of Meat Products: Integration of Accelerated Sample Preparation, Recombinase Polymerase Amplification, and Test-Strip Detection // Molecules. 2021. Vol. 26. No. 22. p. 6804.
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TY - JOUR
DO - 10.3390/molecules26226804
UR - https://doi.org/10.3390%2Fmolecules26226804
TI - Rapid Full-Cycle Technique to Control Adulteration of Meat Products: Integration of Accelerated Sample Preparation, Recombinase Polymerase Amplification, and Test-Strip Detection
T2 - Molecules
AU - Popravko, Demid S
AU - Zvereva, Elena A.
AU - Ivanov, Aleksandr V.
AU - Safenkova, I.V.
AU - Dzantiev, Boris B.
AU - Zherdev, Anatoly V.
PY - 2021
DA - 2021/11/11 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 6804
IS - 22
VL - 26
PMID - 34833896
SN - 1420-3049
ER -
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@article{2021_Ivanov,
author = {Demid S Popravko and Elena A. Zvereva and Aleksandr V. Ivanov and I.V. Safenkova and Boris B. Dzantiev and Anatoly V. Zherdev},
title = {Rapid Full-Cycle Technique to Control Adulteration of Meat Products: Integration of Accelerated Sample Preparation, Recombinase Polymerase Amplification, and Test-Strip Detection},
journal = {Molecules},
year = {2021},
volume = {26},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {nov},
url = {https://doi.org/10.3390%2Fmolecules26226804},
number = {22},
pages = {6804},
doi = {10.3390/molecules26226804}
}
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MLA
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Ivanov, Aleksandr V., et al. “Rapid Full-Cycle Technique to Control Adulteration of Meat Products: Integration of Accelerated Sample Preparation, Recombinase Polymerase Amplification, and Test-Strip Detection.” Molecules, vol. 26, no. 22, Nov. 2021, p. 6804. https://doi.org/10.3390%2Fmolecules26226804.