volume 41 issue 3-4 pages 147-150

The use of commercial pectinase in fruit juice industry. Part I: viscosimetric determination of enzyme activity

Publication typeJournal Article
Publication date1999-08-01
scimago Q1
wos Q1
SJR1.159
CiteScore11.8
Impact factor5.8
ISSN02608774, 18735770
Food Science
Abstract
The kinetics of commercial pectinase, Pectinex Ultra SP-L, in a batch reactor was studied. The change in viscosity of pectin solution was followed by an enzymatic assay to determine pectolytic activity of the enzyme preparation. The pectolytic activity of Pectinex Ultra SP-L was measured at pH 3.5 and 35°C and found to be 0.025 pectin % (w/v)/sec/enzyme % (v/v). The Michaelis-Menten constant (Km) of enzyme preparation was found to be 1.137 pectin % (w/v). The temperature dependence of the reaction rate was obeying the Arrhenius Law. The activation energy of the biochemical reaction catalysed by commercial pectinase was calculated as 9.316 kcal mol−1.
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GOST |
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GOST Copy
Mutlu M. et al. The use of commercial pectinase in fruit juice industry. Part I: viscosimetric determination of enzyme activity // Journal of Food Engineering. 1999. Vol. 41. No. 3-4. pp. 147-150.
GOST all authors (up to 50) Copy
Mutlu M., Sarioglu K., Demir N., ERCAN M. T., Acar J. The use of commercial pectinase in fruit juice industry. Part I: viscosimetric determination of enzyme activity // Journal of Food Engineering. 1999. Vol. 41. No. 3-4. pp. 147-150.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1016/s0260-8774(99)00088-6
UR - https://doi.org/10.1016/s0260-8774(99)00088-6
TI - The use of commercial pectinase in fruit juice industry. Part I: viscosimetric determination of enzyme activity
T2 - Journal of Food Engineering
AU - Mutlu, Mehmet
AU - Sarioglu, Kemal
AU - Demir, Nilay
AU - ERCAN, MERAL T.
AU - Acar, Jale
PY - 1999
DA - 1999/08/01
PB - Elsevier
SP - 147-150
IS - 3-4
VL - 41
SN - 0260-8774
SN - 1873-5770
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{1999_Mutlu,
author = {Mehmet Mutlu and Kemal Sarioglu and Nilay Demir and MERAL T. ERCAN and Jale Acar},
title = {The use of commercial pectinase in fruit juice industry. Part I: viscosimetric determination of enzyme activity},
journal = {Journal of Food Engineering},
year = {1999},
volume = {41},
publisher = {Elsevier},
month = {aug},
url = {https://doi.org/10.1016/s0260-8774(99)00088-6},
number = {3-4},
pages = {147--150},
doi = {10.1016/s0260-8774(99)00088-6}
}
MLA
Cite this
MLA Copy
Mutlu, Mehmet, et al. “The use of commercial pectinase in fruit juice industry. Part I: viscosimetric determination of enzyme activity.” Journal of Food Engineering, vol. 41, no. 3-4, Aug. 1999, pp. 147-150. https://doi.org/10.1016/s0260-8774(99)00088-6.